The monoterpene compounds for juvenile hormone activity through changes in pattern of chitin deposition in the integument of fifth instar larvae of silkworm, Bombyx mori (L) (PM x CSR2)
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The insects are a class of invertebrates within the arthropod phylum that have a chitinous exoskeleton. The leaf eating insects obtain their nutrients and growth promoting biocompounds from the variable or specific flora available for them. The plants on earth are the richest source of metabolites including juvenile hormone analogues for leaf eating insects like silkworm, Bombyx mori (L). Some of plant origin metabolites are acting as insects juvenoids for insect lives. They serve to take pause in the progression of metamorphosis through arresting some of the biochemical reactions including chitin synthesis or accelerating progression through other biochemical pathways in the larval body of insects. The ten microliters of various concentrations of acetone solution of Fernasol Methyl Ether (FME) and each selected monoterpene compounds (Myrcene; Camphene; Cymene; Limonene and Eucalyptol ) were used for topical application to individual larval instars of silkworm, Bombyx mori (L) (Race: PM x CSR2) at 48 hours after the fourth moult. The integument chitin of untreated control larvae; acetone treated control; FME treated larvae and monoterpene treated larvae was estimated at 120 hours after the fourth moult. Topical application of selected concentrations of acetone solutions of selected monoterpenes to fifth instar larvae of silkworm, Bombyx mori (L) (Race: PM x CSR2) was found reflected into the reduction in the deposition of chitin in the larval body wall. The reduction in body wall chitin was found ranging from zero to hundred percent. The plot of concentrations of acetone solutions of FME and monoterepene compounds and percent reduction in the body wall chitin was found exhibiting a characteristic Sigmoid form of displacement, which herewith titled as “Punyamayee Baramati Dose Response Curve”. Since the effects of juvenoids involve the inhibition of metamorphosis through reduction in chitin deposition, it is possible to express the concentration (dose) applied in terms of ID50 value. The ID50 value of juvenoid contents of FME and selected monoterpene compounds can be defined as the specific unit (microgram), which enable to chitin to deposit fifty percent less in the body wall of larvae (In comparison with untreated control). Accordingly, the ID50 value calculated from the “Punyamayee Baramati Dose Response Curves” for FME was found measured 0.08 mg/ml. The ID50 values for monoterpene compounds: Myrcene; Camphene; Cymene; Limonene and Eucalyptol were found measured: 0.116; 0.122; 0.164; 0.172 and 0.208 mg/ml respectively. Acetone soluble juvenoid content of terpene compounds may be utilized efficiently for the fortified development of fifth instars of silkworm, Bombyx mori (L) and thereby, the cocoon quality. Sigmoid (S-form) “Baramati Dose Response Curve” may help for quantitative estimation of juvenoid contents of various terpene compounds and terpenoids.
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