SYNTHESIS AND POTENTIAL CYTOTOXICITY EVALUATION OF CARBOXYMETHYL CHITOSAN HYDROGELS

• Authors: Karol K Kłosiński , Małgorzata K. Girek Girek , Radosław A. Wach , Renata Czechowska-Biskup , Piotr T. Arkuszewski , Paweł B Szymański , Zbigniew Pasieka
• Languages of publication: EN

Abstracts

EN

The aim of the research was to employ radiation to produce flexible carboxymethyl chitosan (CMCS) based hydrogels of uniform structure to characterise their swelling properties and cytocompatibility for potential applications as hydrogel wound dressings. CMCS in aqueous solution was irradiated with an electron beam in the presence of a poly(ethylene glycol) diacrylate (PEGDA) macromonomer as a crosslinker, at 12 different compositions, i.e. 3–20% CMCS, 3 and 5% PEGDA. The obtained hydrogels were subjected to sol–gel analysis. The amount of insoluble fraction (up to 100%) rose with an increase in the PEGDA/polysaccharide ratio. Moreover, the equilibrium degree of swelling, ca. 15 to 200 g of water per g of gel, which was higher for lower content of crosslinker, decreased with the delivered dose, which was associated with an increase in crosslinking density. The in vitro XTT cell viability assay (murine fibroblasts, L929 cell line) showed no significant cytotoxicity of CMCS gels.

Keywords

EN

PEGDA; XTT; carboxymethyl chitosan; cytotoxicity; hydrogel; radiation crosslinking

• Publisher: Sieć Badawcza Łukasiewicz - Polskie Towarzystwo Chitynowe
• Journal: Progress on Chemistry and Application of Chitin and its Derivatives
• Year: 2017
• Volume: 22
• Pages: 82-96

Contributors

author

Karol K Kłosiński

• Department of Experimental Surgery, Faculty of Medicine, Medical University of Lodz, Pabianicka 62, 93-513 Lodz, Poland

author

Małgorzata K. Girek Girek

• Department of Pharmaceutical Chemistry, Drug Analysis and Radiopharmacy, Faculty of Pharmacy, Medical University of Lodz, Muszyńskiego 1, 90-151 Lodz, Poland

author

Radosław A. Wach

• Institute of Applied Radiation Chemistry, Faculty of Chemistry, Lodz University of Technology, Wroblewskiego 15, 93-590 Lodz, Poland.

author

Renata Czechowska-Biskup

• Institute of Applied Radiation Chemistry, Faculty of Chemistry, Lodz University of Technology, Wroblewskiego 15, 93-590 Lodz, Poland.

author

Piotr T. Arkuszewski

• Institute of Applied Radiation Chemistry, Faculty of Chemistry, Lodz University of Technology, Wroblewskiego 15, 93-590 Lodz, Poland.

author

Paweł B Szymański

• Department of Pharmaceutical Chemistry, Drug Analysis and Radiopharmacy, Faculty of Pharmacy, Medical University of Lodz, Muszyńskiego 1, 90-151 Lodz, Poland

author

Zbigniew Pasieka

• Institute of Applied Radiation Chemistry, Faculty of Chemistry, Lodz University of Technology, Wroblewskiego 15, 93-590 Lodz, Poland.

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• Document Type: article