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2021 | 34 | 29-37
Article title

Presence of CL influence the quantity and quality of COC in slaughter house derived ovaries of Boran heifers

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The objective of the study was to determine whether the presence of CL on the ovary can influence the number and quality of COCs recovered using needle aspiration technique. A total 40 numbers of Bovine ovaries were collected from the local abattoirs soon after the animals were slaughtered. The specimens were classified as corpus luteum present (CL+) and corpus luteum absent (CL) groups. A total of 583 follicles were aspirated from 40 ovaries and among them 381 (62.5%) COCs were collected from CL group ovaries and 202 (37.5%) COCs from CL+ group ovaries. The COCs were also classified as Grade I (>3 layers of cumulus), Grade II (2 or 3 layers of cumulus), Grade III (no cumulus) and Grade IV (degenerated cells). Grade I and Grade II were classified as normal, and Grade III and Grade IV were considered as abnormal COCs. The result indicated that greater numbers of follicles were aspirated and COCs were collected from CL ovaries (15.24±5.27 and 9.40±3.50, respectively) than ovary with CL+. Ovaries CL contribute higher normal COCs (Grades I and II) than that of ovaries with CL+. The result of this study is a preliminary work directing suitable source of COCs for initiating and optimizing in vitro embryo production experiment in like Ethiopia having huge cattle population and animal slaughtering is more common for various reasons like to export or for domestic consumption.
Physical description
  • Debre Zeit Agricultural Research Center, Ethiopian Institute of Agricultural Research, Ethiopia
  • Debre Zeit Agricultural Research Center, Ethiopian Institute of Agricultural Research, Ethiopia
  • College of Veterinary Medicine and Agriculture, Addis Ababa University, Ethiopia
  • Department of Animal Science, Iowa State University, Ames, IA 50011, USA
  • [1] Alberro, M. & Haile-Mariam, S. (1982). The indigenous cattle of Ethiopia. Part I. World Anim. Rev. 41, 2-10
  • [2] Asad L.Y. (2015). Effect of bovine serum albumin and follicular fluid on in vitro maturation, fertilization and development of goat embryos. PhD Thesis. Department of Animal Breeding and Genetics, Bangladesh Agricultural University.
  • [3] Cetica P.D., Pintos L.N., Dalvit G.C., Beconi M.T. (1999). Effect of lactate dehydrogenase activity and iso enzyme localization in bovine oocytes and utilization of oxidative substrates on in vitro maturation. Theriogenology, 51(3): 541-50
  • [4] Duygu B.A., Muhammed K.B., Dogan N., and G. Hander. (2013). Effect of the stage of oestrus cycle on follicular population oocyte yield and quality, and biochemical composition of serum and follicular fluid in Anatolian water buffalo. Animal Reproduction Science, 137, 3-4, 8-14
  • [5] Farahavar A., Shahne A.Z. (2010). Effect of melatonin on in vitro maturation of bovine oocytes. African Journal of Biotechnology 9(17): 2579-2583
  • [6] Ginther O.J., D.R. Bergfelt, L.J. Kulick and K. Kot. (1996). Selection of the dominant follicle in cattle: role of two-way functional coupling between follicle stimulating hormone and the follicles. Biol Reprod. 62: 920-927
  • [7] Hafez E.S.E. (1993). Reproduction in Farm Animals. Fifth Edition. Lea and Febriger: Philadelphia.
  • [8] Hammad M.E., Gabr S.A., El-Ratel I.T., Gad M.A. (2014). Efficacy of different collection techniques on yield and quality of Egyptian buffalo oocytes. J Anim Poultry Prod Mansoura University 5: 413-22
  • [9] Islam M.R., Mamy Khandoker, S. Afroz, M.G.M. Rahman and R.I. Khan (2007). Qualitative and quantitative analysis of goat ovaries, follicles and oocytes in view of in vitro production of embryos. J. Zhejiang University 8: 465-469
  • [10] Khandoker Mamy, K. Imai, T. Takahashi and K. Hashizume (2001). Role of gelatinase on follicular atresia in the bovine ovary. Biol. Reprod. 65: 726-732
  • [11] Kouamo J., Tidjou S.G.D., Zoli, A.P. and Y.M. Mfopit (2015). Effect of nutritional status on the ovarian follicular population, yield and quality of oocytes in the Ngaoundere gudali zebu (Bos indicus). Veterinary World, 8, 4, 502-507
  • [12] Kumar N., S. Paramasivan, P. Sood and M. Singh (2004). Micrometry of different category oocytes recovered from goat ovaries. Ind. J. Anim. Sci. 74: 259-260.
  • [13] Mmmy, K., Atiqah, N., & Ariani, N. (2017). Effect of ovarian types and collection techniques on the number of follicles and the quality of cumulus-oocyte-complexes in cow. Bangladesh Journal of Animal Science, 45(3), 10-16.
  • [14] Mondal M.A., Mamy Khandoker, M.A. Mondal, A.H.M.S. Rahman, A.S. Apu and S. Pervage (2008). In vitro production of goat embryos in Bangladesh. Bang. J. Anim. Sci. 37: 1-9
  • [15] Nandi S., M.S. Chauhan and P. Palta (2000). Effect of a corpus luteum on the recovery and developmental potential of buffalo oocytes. Vet. Res. 147: 580-581
  • [16] Pontes J. H. F., Silva K. C. F., Basso A. C., Ferreira C. R., Santos G. M. G., Sanches B. V., Porcionato J. P. F., Vieira P. H. S., Sterza F. A. M., Seneda M. M. (2009). 179 Comparison of oocyte and embryo production among Bos taurus, Bos indicus and Indicus taurus donor cows. Reproduction, Fertility and Development 22(1) 248-248
  • [17] Saha S., Mamy Khandoker, L.Y. Asad, A.M.M.T. Reza and A. Hoque (2014). Effect of fresh and frozen semen on in vitro fertilization and subsequent development of goat embryos. Iran. J. Appl. Anim. Sci. 4: 325-330
  • [18] Sianturi R.G., Thein M., Wahid H., Rosnina Y.C. (2002). Effect of collection technique on yield of bovine oocytes and the development potential of oocytes from different grades of oocytes. Indonesian Journal of Animal and Veterinary Sciences 7(3): 188-193
  • [19] Webb R., B.K. Campbell, H.A. Garveric and J.G. Gong (1999). Molecular mechanisms regulating follicular recruitment and selection. J. Reprod. Fert. 45: 123-126
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