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2016 | 32 | 84-94
Article title

Implications of Plant Growth Regulators on Induction of In vitro flowering in Aerva lanata (L.) Juss. ex Schult.

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Abstracts
EN
In vitro flowering was successfully induced from the shoots regenerated in cultures of Aerva lanata. In vitro flowering method can be monitored effectively in studying floral transition and flower development from vegetative to reproductive phase. The nodal shoot segments were used as explants in the present study. The explants were surface sterilized using HgCl2 and inoculated on Murashige and Skoog’s (MS) medium supplemented with 6-benzylaminopurine (BAP) for bud break. Maximum numbers of shoots were induced on MS medium supplemented with 1.0 mg l-1 BAP. The cultures were multiplied by continuous subculturing of shoots with mother explants on fresh MS medium with BAP and Kinetin (Kin). Rooting was achieved on one fourth MS medium supplemented with 2.0 mg l-1 indole-3-butyric acid (IBA). The multiplied shoots were subjected to various concentrations of different plant growth regulators and photoperiod regimes to induce flowering in vitro. The maximum numbers of in vitro flowers were recorded from the shoots on full strength MS medium with 0.5 mg l-1 BAP and 0.1 mg l-1 indole-3-acetic acid (IAA) on 12/12 h/d photoperiod.
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Year
Volume
32
Pages
84-94
Physical description
Contributors
author
  • Department of Plant Science, M.G.G.A.C. Mahe, Pondicherry, India
  • Department of Plant Science, M.G.G.A.C. Mahe, Pondicherry, India
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Document Type
article
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YADDA identifier
bwmeta1.element.psjd-ec12442a-00fb-4f48-b74a-6d8607d71343
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