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2011 | 16 | 61 - 70
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Biodegradable nanoparticulated carriers have important potential applications for administration of therapeutic proteins and peptides. Chitosan based nanoparticles have attracted attention due to their biological properties such as biodegrability and biocompatibility. However, chitosan nanoparticles are not stable in colloid form. Therefore, to increase the stability of these particles is an important problem. The aim of this work was to obtain stable nanoparticles formed by N-acyl derivatives of chitosan by ionic gelation and to investigate their ability to interact with acidic, neutral and basic proteins.
61 - 70
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  • Centre «Bioengineering» RAS
  • Centre «Bioengineering» RAS
  • Centre «Bioengineering» RAS
  • Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry of RAS
  • Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry of RAS
  • Centre «Bioengineering» RAS
  • Angelova N., Hunkeler D.; (1999). Rationalizing the design of polymeric biomaterials.TIBTECH, Vol. 17, pp. 409-421.
  • Lim, C.K., & Ahmad, S.H. (2009). In vitro models in biocompatibility assessment for biomedicalgrade chitosan derivatives in wound management. Int. J. Mol. Sci., Vol. 10, pp. 1300-1313.
  • Yen M.-T., Yang J.-H., Mau J.-L.; (2009). Phisicochemical characterization of chitin and chitosan from crab shells. Carbohydrate Polymers, Vol. 75, pp. 15-21.
  • Rekka M. R., Sharma C. P.; (2009). Synthesis and evaluation of lauryl succinyl chitosan particles towards oral insulin delivery and absorption. J. Controlled Release, Vol. 135, pp. 144-151.
  • Ottenbrite R. M.; (2006). Perspectives on: Chitosan drug delivery systems based on their geometries. J. Bioactive and Compatible Polymers, Vol. 21, pp. 351-368.
  • Rieux A., Fievez V., Garinot M., Schneider Y-J., Preat V.; (2006). Nanoparticles as potential oral delivery systems of proteins and vaccines: a mechanistic approach. J. Controlled Release, Vol. 116, pp. 1-27.
  • Calvo P., Remunan-Lopez C., Vita-Jato J. L., Alonso M. J.; (1997). Novel hydrophilic chitosanpolyethylene oxide nanoparticles as protein carriers. J. Appl. Polym. Sci., pp. 63, pp. 125-132.
  • Il’ina, A.V., & Varlamov, V.P. (2004). Hydrolysis of chitosan in lactic acid. Applied Biochemistry and Microbiology, Vol. 40, pp. 300-303.
  • Kulikov S. N., Chirkov S. N., Il’ina A.V., Lopatin S.A., Varlamov V. P.; (2006). Effect of the molecular weight of chitosan on its antiviral activity in plants. Applied Biochemistry and Microbiology, Vol. 42, pp. 200-203.
  • Il’ina A. V., Varlamov V. P.; (2003). Effect of the degree of acetylation of chitosan on its enzymatic hydrolysis with the preparation Celloviridin G20x. Applied Biochemistry and Microbiology, Vol. 39, pp. 239-242.
  • Hirai A., Odani H., Nakajima A.; (1991). Determination of Degree of Deacetylation of Chitosan by 1H NMR Spectroscopy Polym. Bull. Vol. 26, pp. 87-94.
  • Il’ina A. V., Varlamov V. P., Ermakov Yu. A., Orlov V. N., Skryabin K. G.; (2008). Chitosan is a Natural Polymer for Constructing Nanoparticles. Doklady Chemistry, Vol. 421, pp. 165-167.
  • Tikhonov V. E., Lopez-Llorca L. V., Salinas J., Monfort E.; (2004). Endochitinase activity determination using N-fluorescein-labeled chitin. J Biochem. Biophys. Methods, Vol. 60, pp. 29-38.
  • Twinning S.S.; (1984). Fluorescent isothiocyanate-labeled casein assay for proteolytic enzymes. Anal.Biochem., Vol. 143, pp. 30-34.
  • Lopez-Leon T., Carvalho E. L. S., Seijo B., Ortega-Vinuesa J. L., Bastos-Gonzalez D.; (2005). Physicochemical characterization of chitosan nanoparticles. J. Colloid Interface Sci., Vol. 283, pp. 344-351.
  • Zhang J., Chen X. G., Li Y. Y., Liu C. S.; (2007). Self-assembled nanoparticles based on hydrophobically modified chitosan as carriers for doxorubicin. Naomedicine, Vol. 3, pp. 258-265.
  • Lin Y-H., Mi F.-L., Chen C.-T.; (2007). Preparation and characterization of nanoparticles shelled with chitosan for oral insulin delivery. Biomacromolecule, Vol. 8, pp. 146-152.
  • Tang Z-X., Shi L-E.; (2007). Adsorption of neutral proteinase on chitosan nano-particles. Biotechnol. & Biotechnol. EQ., Vol. 21, pp. 223-228.
  • Kurchenko V., Gavrilenko N., Halavach T., Cherviakovsky E., Bakulin A., Albulov A.; (2009). Proteolysis of whey proteins after complex formation with chitosan. Conference Book 9th Internat. Conf. of the European Chitin Society (pp. 104-105), Venice-Italy.
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