Phytochemical contents and antioxidant potentials of Sorghum bicolor (L.) Moench (Sorghum), Hibiscus sabdariffa L. (Roselle) and their varying blends

• Authors: O. A. Adediran , P. Adigun , A. A. Ayankunle , A. S. Oyegunwa
• Languages of publication: EN

Abstracts

EN

The use of natural ingredients in food products has gained wide acceptance because of their health benefits for both humans. Sorghum bicolor and Hibiscus sabdariffa possess abundant phytochemicals and bioactive compounds that promote health and wellness. This research was carried out to examine the phytochemical content and antioxidant activities of Sorghum bicolour, Hibiscus sabdariffa L., and their blends The leaf sheaths of Sorghum bicolor and calyces of Hibiscus sabdariffa were procured, dried till constant weight, then pulverised to assess their phytochemical compositions qualitatively and quantitatively. In addition to undiluted sorghum and roselle, blends comprising 75% Sorghum + 25% Roselle, 25% Sorghum + 75% Roselle, and 50% Sorghum + 50% Roselle were derived, to make a total of five treatments for evaluation. The DPPH activities of the plants and their blends were also assessed. The results showed that saponin, alkaloids, flavonoid, tannin and reducing sugar were found in the blends. There were significant (p<0.05) differences in phytochemical composition of Sorghum bicolor, Roselle and their different blends. Tannin and Saponin contents were significantly (p<0.05) highest (125.67, 65.19, respectively) in 75% Sorghum + 25% Roselle and lowest in 100%Roselle (85.42) and 100% Roselle (52.19). Alkaloid and reducing sugar followed the same trend with 100% Roselle having significantly (p<0.05) higher values (59.12, 12.89), while 75% Sorghum + 25% Roselle had the lowest value of 40.76, and 7.82respectively. Flavonoid was significantly (p<0.05) the highest (82.31) in 50% Sorghum + 50% Roselle and lowest in 75% Sorghum + 25% Roselle. The antioxidant activity measured via DPPH was significantly different (p<0.05) across treatments with 50% Sorghum + 50% Roselle recording the highest (66.53), 100%Sorghum had the lowest (49.69), while 100% Rosselle, 75% Sorghum + 25% Roselle and 50% Sorghum + 50% Roselle had values of 65.20, 64.13 and 57.20 respectively. The combination of Sorghum bicolor and roselle blends presents greater antioxidative properties, with great potentials for the reducing lipid oxidation and microbial activities that can lead to food spoilage, thus improving the shelf life of food and ensuring the safe consumption of such food products in which they are embedded.

Keywords

EN

Hibiscus sabdariffa; Sorghum bicolor; antioxidant; blends; phytochemical; roselle; sorghum

Discipline

• BIOCHEMISTRY: BIOCHEMISTRY

• Publisher: Scientific Publishing House „DARWIN”
• Journal: World News of Natural Sciences
• Year: 2025
• Volume: 61
• Issue: 2
• Pages: 312-319

Contributors

author

O. A. Adediran

• Department of Animal Science, University of Ibadan, Ibadan, Nigeria

author

P. Adigun

• Department of Animal Science, University of Ibadan, Ibadan, Nigeria

author

A. A. Ayankunle

• Department of Animal Science, University of Ibadan, Ibadan, Nigeria

author

A. S. Oyegunwa

• Department of Animal Science, Tai Solarin University of Education Ijagun, Nigeria

References

• [1] A.O.A.C. (1990). Official Methods of Analysis. 15th Edition, Association of Official Analytical Chemist, Washington DC.
• [2] Adamu, J., Jhonson, R., Nilcox, C. F. (1970). Laboratory experiments, inorganic chemistry. The Macmillan Company, London, UK. 172 pages.
• [3] Ajuru, M. G., and Williams, L. F. (2017). Qualitative and Quantitative Phytochemical Screening of Some Plants Used in Ethnomedicine in the Niger Delta Region of Nigeria. Journal of Food and Nutrition Sciences 5(5): 198-202
• [4] Ali, B. H., Mousa, H. M., and El‐Mougy, S. (2005). The effect of a water extract and anthocyanins of Hibiscus sabdariffa L. on paracetamol‐induced hepatoxicity in rats. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives 17(1), 56-59
• [5] Awika, J. M., and Rooney, L. W., (2004). Sorghum phytochemicals and their potential impact on human health. Phytochemistry 65, 1199-1221
• [6] Awika, J. M., and Rooney, L. W. (2005). Products and health benefits of Specialty sorghum phytochemicals and their potential impact on human health. Phytochemistry. Journal of Cereal Foods World 50, 109-115
• [7] Banwo, K., Sanni, A., Sarkar, D., Ale, O., & Shetty, K. (2022). Phenolics-Linked Antioxidant and Anti-hyperglycemic Properties of Edible Roselle (Hibiscus sabdariffa Linn.) Calyces Targeting Type 2 Diabetes Nutraceutical Benefits in vitro. Frontiers in Sustainable Food Systems 6, 660831. https://doi.org/10.3389/fsufs.2022.660831
• [8] Blois, M.S. (1958). Antioxidant Determinations by the Use of a Stable Free Radical. Nature 181, 1199-1200
• [9] Dykes, L., and Rooney, L.W. (2006). Sorghum and millet phenols and antioxidants. Journal of Cereal Science 44(3), 236-251
• [10] Dykes, L. and Rooney, W. (2007). Phenolic Compounds in Cereal Grains and Their Health Benefits. Journal of Cereal Foods World 52, 105-111
• [11] Iruoghene, E. G., Oghenekeno, P., Jikah, A., Oloni, G. O., Ajokpaoghene, M. O., and Essaghah, A. E. (2023). Proximate composition and health benefits of Roselle leaf (Hibiscus Sabdariffa L.,). Insight on food and health benefits. Journal of Food chemistry Advances 3, 100-107
• [12] Harborne, J. B. (1998). Textbook of Phytochemical Methods. A Guide to Modern Techniques of Plant Analysis. 5th Edition, Chapman and Hall Ltd, London, 21-72.
• [13] McDonald, S., Prenzler, P.D., Autolovich, M. and Robards, K. (2001). Phenolic Content and Antioxidant Activity of Olive Extracts. Food Chemistry 73, 73-84
• [14] Moyo, M., Stephen, A., Ncube, B., Ashwell, R. N. (2013). Phytochemical and antioxidant properties of unconventional leafy vegetables consumed in Southern Africa. South Africa Journal of Botany 84, 65-71
• [15] Parekh, J., and Chanda, S. (2007). Antibacterial and phytochemical studies on twelve species of Indian medicinal plants. African Journal of Biomedical Research 10(2), 54-58.
• [16] Polshettiwar, S. A., Ganjiwale, R. O., Wadher, S. J. and Yeole, P. G. (2007). Spectrophotometric estimation of total tannins in some ayurvedic eye drops. Indian Journal of Pharmaceutical Sciences 69 (4), 22-27
• [17] Stoilova, I., Krastanov, A. I., Stoyanova A. and Panteley, D. (2007). Antioxidant activity of ginger extract (Zingiber officianale). Food Chemistry. 102(3), 764-770
• [18] Tsai, Y., Wan-Chun, L., Du, Y., Shou-Fang, W., El-Shazly, M., Lee, C., Yen, M., Ming-Feng, H., Yang-Chang, W., and Fang-Rong, C. (2012). Lignan and flavonoid phytoestrogens from the seeds of Cuscuta chinensis. .Journal of Natural Products 7, 1424
• [19] Yang, Y.H., Wan, H., and Liao, F. L. (2005). Effects of Plant Allelochemicals on Seed Germination. Journal of Ecology, 24, 1459-1465
• [20] Yanishilieva, N. V., Marionova, E., Pokorny, J., (2006). Natural antioxidants from herbs and spices. European Journal of Lipid Science and Technology, 108, 776-793

• Document Type: article