THE EFFECT OF CHITOSAN ON THE SYNTHESIS OF L-S-NITROSOCYSTEINE THAT PARTICIPATES IN THE REGULATION OF THE M2 PYRUVATE KINASE ISOENZYME ACTIVITY ASSOCIATED WITH EHRLICH ASCITES CELLS PROLIFERATION IN VITRO

• Authors: Jan Ignacak , Jacek Zagajewski , Iwona Pałka , Maria Wiśniewska-Wrona , Antoni Niekraszewicz
• Languages of publication: EN

Abstracts

EN

L-S-nitrosocysteine formation in EAT tumor cells and normal CRL-1636 cells incubated with microcrystalline chitosan was confirmed by RP-HPLC. The metabolite was identified based on UV-VIS spectra. The formation of L-S-nitrosocysteine in EAT tumor cells contributes to decreasing the level of L-cysteine in these cells. L-cysteine as an effector of the bifunctional M2 isoenzyme of pyruvate kinase (PK) initiates its histone kinase activity, which is responsible for histone H1 phosphorylation. A decrease of L-cysteine level in EAT tumor cells contributes to lack of histone H1 phosphorylation by the M2 PK isoenzyme and by the same token to inhibition of EAT cell proliferation.

Keywords

EN

L-S-nitrosocysteine; L-cysteine; inhibition of EAT cell proliferation; microcrystalline chitosan; pyruvate kinase M2 isoenzyme

• Publisher: Sieć Badawcza Łukasiewicz - Polskie Towarzystwo Chitynowe
• Journal: Progress on Chemistry and Application of Chitin and its Derivatives
• Year: 2010
• Volume: 15
• Pages: 149 - 158

Contributors

author

Jan Ignacak

• Chair of Medical Biochemistry, Collegium Medium, Jagiellonian University

author

Jacek Zagajewski

• Chair of Medical Biochemistry, Collegium Medium, Jagiellonian University

author

Iwona Pałka

• Chair of Medical Biochemistry, Collegium Medium, Jagiellonian University

author

Maria Wiśniewska-Wrona

• Institute of Biopolymer and Chemical Fibres

author

Antoni Niekraszewicz

• Institute of Biotechnology and Chemical Fibers

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• Document Type: article