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2013 | 67 | 6 | 374–379
Article title

Ocena wybranych populacji limfocytów oraz stężeń immunoglobulin u pracowników oddziałów kardiologii interwencyjnej wykorzystujących niskie dawki promieniowania jonizującego w procedurach kardiologicznych

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Title variants
EN
Evaluation of selected population of lymphocytes and concentrations of immunoglobulins in workers of interventional cardiology wards using low doses of ionizing radiation in cardiovascular procedures
Languages of publication
PL
Abstracts
EN
INTRODUCE The aim of the study was to determinate the effect of low X-ray doses on the percentage and number of peripheral blood lymphocytes T (CD3+), T helper (CD4+), T cytotoxic (CD8+), lymphocytes B (CD19+), activated T lymphocytes (CD3+CD25+) and activated T helper lymphocytes (CD4+CD25+) as well as serum immunoglobulins (IgM, IgG and IgA) concentration in workers of an interventional cardiology ward. MATERIAL AND METHODS The study was performed among a group of 40 workers (28 women and 12 men) and a control group of 35 persons (25 women and 10 men). Flow cytometry was used for the analysis of lymphocytes and the turbidimetric method for assessing serum immunoglobulin concentrations. RESULTS The study showed a decreased percentage and number of CD3+CD25+ and CD4+CD25+ cells as well as IgG concentration in interventional cardiology ward staff in comparison to the control group. A negative correlation between the length of employment and percentage of CD3+CD25+ was found. CONCLUSION The study indicates suppressive impact low doses of ionizing radiation on activated T cells, activated T helper cells as well as IgG production that is adverse in relation to the existing risk of infections caused by different microorganisms.
PL
WSTĘP Celem pracy była ocena oddziaływania niskich dawek promieniowania jonizującego na odsetek i liczbę limfocytów T (CD3+), limfocytów T pomocniczych (CD4+), limfocytów T cytotoksyczych (CD8+), limfocytów B (CD19+), aktywowanych limfocytów T (CD3+CD25+) i aktywowanych limfocytów T pomocniczych (CD4+CD25+) oraz stężeń immunoglobulin IgM, IgG i IgA u pracow-ników oddziału kardiologii interwencyjnej. MATERIAŁ I METODY Badanie przeprowadzono w grupie 40 zatrudnionych (28 kobiet i 12 mężczyzn) oraz w grupie kontrolnej 35 osób (25 kobiet i 10 mężczyzn). Badania limfocytów krwi obwodowej przeprowadzono z wykorzystaniem cytometrii przepływowej, a stężenia immunoglobulin w surowicy oznaczono metodą turbidymetryczną. WYNIKI W badanej grupie pracowników – w porównaniu z grupą kontrolną – wykazano znamienne obniżenie liczby i odsetka limfocytów CD3+CD25+ i CD4+CD25+ oraz stężeń immunoglobuliny IgG. Wykazano także istnienie negatywnej korelacji między okresem zatrudnienia badanych pracowników a odsetkiem limfocytów CD3+CD25+. WNIOSEK Badanie wskazuje na supresyjne oddziaływanie niskich dawek promieniowania jonizującego na aktywowane limfocyty T, aktywowane limfocyty T pomocnicze i produkcję IgG, co nie jest korzystne w odniesieniu do istniejącego ryzyka zakażeń drobnoustrojami.
Discipline
Year
Volume
67
Issue
6
Pages
374–379
Physical description
References
  • 1. Stewart C., Stevenson A., Habbersett R. The effect of low-dose irradiation on unstimulated and PHA-stimulated human lymphocyte subsets. Int. J. Radiat. Biol. Relat. Stud. Phys. Chem. Med. 1988; 53: 77–87.
  • 2. Gridley D., Pecaut M., Nelson G. Total-body irradiation with high-LET particles: acute and chronic effects on the immune system. Am. J. Physiol. Regul. Integr. Comp. Physiol. 2002; 282: R677–R688.
  • 3. Hrycek A., Stieber M. Selected problems concerning X-ray influence on leukocytes. Wiad. Lek. 1994; 47: 288–299.
  • 4. Maffei F., Angelini S., Cantelli F.G. i wsp. Spectrum of chromosomal aberrations in peripheral lymphocytes of hospital workers occupationally exposed to low doses of ionizing radiation. Mutat. Res. 2004; 547: 91–99.
  • 5. Zakeri F., Hirobe T., Akbari Noghabi K. Biological effects of low-dose ionizing radiation exposure on interventional cardiologists. Occup. Med. 2010; 60: 464–469.
  • 6. Facchini A., Maraldi N., Bartoli S., Farulla A., Manzoli F. Changes in membrane receptors of B and T human lymphocytes exposed to 60Co gamma rays. Radiat. Res. 1976; 68: 339–348.
  • 7. Herrera M., Diaz-Perches R., Gutierrez M. i wsp. The inhibitory effect of ionizing radiation on Fc and C3 receptors on mouse and human leukocytes, and the protective potential of human albumin. Exp. Hematol. 1990; 18: 853–856.
  • 8. Godekmerdan A., Ozden M., Ayar A., Gursu M., Ozan A., Serhatlioglu S. Diminished cellular and humoral immunity in workers occupationally exposed to low levels of ionizing radiation. Arch. Med. Res. 2004; 35: 324–328.
  • 9. Kłuciński P., Mazur B., Kaufman J., Hrycek A., Cieślik P., Martirosian G. Assessment of blood serum immunoglobulin and c-reactive protein concentrations in workers of x-ray diagnostics units. Int. J. Occup. Med. Environ. Health. 2005; 18: 327–330.
  • 10. Jagła M., Cichocka-Jarosz E. Limfocyty T regulatorowe. Alerg. Astma Immun. 2007; 12: 22–29.
  • 11. Dons E.M., Raimondi G., Cooper D.K., Thomson A.W. Induced regulatory T cells: mechanisms of conversion and suppressive potential. Hum. Immunol. 2012; 73: 328–334.
  • 12. Soldatov S.K., Ushakov I.B. Low doses of ionizing radiation and short- and long-term hematologic changes. Med. Tr. Prom. Ekol. 1995; 9: 20–23.
  • 13. Hrycek A., Czernecka-Micińska A., Kłuciński P., Badowski R. Peripheral blood lymphocytes and selected serum interleukins in workers operating X-ray equipment. Toxicol. Lett. 2002; 132: 101–117.
  • 14. Finkel T. Oxygen radicals and signaling. Curr. Opin. Cell Biol. 1998; 10: 248–253.
  • 15. Shankar B., Premachandran S., Bharambe S.D., Sundaresan P., Sainis K.B. Modification of immune response by low dose ionizing radiation: role of apoptosis. Immunol. Lett. 1999; 68: 237–245.
  • 16. Kłucinski P., Wójcik A., Grabowska-Bochenek R. i wsp. Erythrocyte antioxidant parameters in workers occupationally exposed to low levels of ionizing radiation. Ann. Agric. Environ. Med. 2008; 15: 9–12.
  • 17. Cao M., Cabrera R., Xu Y., Liu C., Nelson D. Different radiosensitivity of CD4(+)CD25(+) regulatory T cells and effector T cells to low dose gamma irradiation in vitro. Int. J. Radiat. Biol. 2011; 87: 71–80.
  • 18. Diaconu C.C., Neagu A.I., Lungu R. i wsp. Plasticity of regulatory T cells under cytokine pressure. Roum. Arch. Microbiol. Immunol. 2010; 69: 190–196.
  • 19. Cools N., Ponsaerts P., Van Tendeloo V.F., Berneman Z.N. Regulatory T cells and human disease. Clin. Dev. Immunol. 2007; 2007: 89195.
  • 20. Ozdemir C., Akdis M., Akdis C.A. T regulatory cells and their counter-parts: masters of immune regulation. Clin. Exp. Allergy. 2009; 39: 626–639.
  • 21. Üstünsöz B. Hospital infections in radiology clinics. Diagn. Interv. Radiol. 2005; 11: 5–9.
  • 22. Drop A., Czekajska-Chehab E., Staśkiewicz G. i wsp. Radiology depar-tament as a possible source of bacterial flora cross-transmission. Sepsis 2009; 2: 71–74.
  • 23. Wan Y., Flavell R.A. How diverse – CD4 effector T cells and their functions. J. Mol. Cell. Biol. 2009; 1: 20–36.
  • 24. Jasiulewicz A., Lisowska K.A., Bryl E., Witkowski J.M. Rola limfocytów B w mechanizmach patogenezy wybranych chorób. Forum Med. Rodz. 2011; 5: 37–46.
Document Type
article
Publication order reference
YADDA identifier
bwmeta1.element.psjd-e699ecf7-9261-4c0f-8907-4ac3f9264bcc
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