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2016 | 19 | 4 | 27-33
Article title

Antimicrobial resistance and molecular characteristics of Streptococcus agalactiae isolated from women of reproductive age

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PL
Lekooporność i molekularna charakterystyka Streptococcus agalactiae izolowanych od kobiet w wieku rozrodczym
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Abstracts
EN
Introduction. Streptococcus agalactiae infections are among the most significant causes of neonatal invasive diseases. Proper screening and detection of pregnant women carrying GBS allows intrapartum administration of antibiotic prophylaxis and is an effective measure in preventing transmission of bacteria from mother to newborns. Material and methods. Sixty three bacterial strains were isolated from vaginal swabs from pregnant and nonpregnant women of reproductive age. Species were identified by colony morphology, haemolysis type, Gram staining and SLIDEX® Strepto Plus latex test. Antimicrobial resistance of 56 strains was determined using disk-diffusion method. The presence of molecular resistance determinants was assessed using PCR with specific primers, and capsular types were identified using multiplex PCR. Results. None of the strains were resistant to the first drug of choice, penicillin. A large percentage of isolates (78.6%) were resistant to doxycycline. The prevalence of resistance to macrolides and lincosamides, antibiotics used in women allergic to penicillin, was high. Those results corresponded with PCR tests, as tetM and ermA1 were most frequently detected genes (98.4 and 87.3%, espectively). 7.94% of strains possessed 7 different out of 13 tested genes determining resistance to different groups of antimicrobials. Among the capsular types, Ia, which proved to be associated with the most severe and invasive infections in mothers and neonates, was the most prevalent (65.08%).
PL
Wstęp. Infekcje wywołane przez Streptococcus agalactiae są jednymi z głównych przyczyn chorób inwazyjnych noworodków. Badania przesiewowe w kierunku nosicielstwa paciorkowców z grupy B (GBS) u ciężarnych umożliwiają zastosowanie śródporodowej profilaktyki antybiotykowej w celu zapobiegania przenoszeniu bakterii z matki na noworodka. Materiały i metody. Przebadano 63 szczepy bakterii uzyskane poprzez wymazy pochwowe od ciężarnych i nieciężarnych kobiet w wieku rozrodczym. Bakterie zidentyfikowano na podstawie morfologii kolonii, typu hemolizy, barwienia Grama i testu SLIDEX® Strepto Plus. Profil lekooporności 56 szczepów zbadano metodą dyfuzyjnokrążkową. Występowanie genów warunkujących lekooporność oznaczono techniką konwencjonalnego PCR, natomiast metoda multiplex PCR posłużyła do oznaczenia polisacharydów otoczkowych. Wyniki. Nie stwierdzono oporności na lek pierwszego wyboru, jakim jest penicylina. 78,6% izolatów było opornych na doksycyklinę. Często także stwierdzano oporność na makrolidy i linkozamidy, które są antybiotykami stosowanymi u pacjentek uczulonych na penicylinę. Wyniki te korespondowały z wynikami testów PCR, gdyż geny tetM i ermA1 były najczęściej stwierdzanymi genetycznymi determinantami lekooporności (odpowiednio u 98,4 i 87,3% szczepów). Aż 7,94% szczepów S. agalactiae posiadało 7 spośród 13 testowanych genów warunkujących oporność na różne antybiotyki. Test multiplex PCR wykazał, że najbardziej rozpowszechniony był typ Ia polisacharydów otoczkowych, powiązany z najcięższymi i najpoważniejszymi infekcjami. Został on wykryty u 65,08% szczepów. Wnioski. Pomimo całkowitej wrażliwości na penicylinę, wielooporność szczepów S. agalactiae izolowanych od kobiet w wieku rozrodczym jest powszechna. Oporność na antybiotyki u tych bakterii może być warunkowana przez występowanie więcej niż jeden gen w jednym izolacie.
Publisher

Year
Volume
19
Issue
4
Pages
27-33
Physical description
Contributors
  • Department of Microbiology, University of Agriculture in Cracow
  • Centre for Microbiological Research and Autovaccines, Cracow
  • Centre for Microbiological Research and Autovaccines, Cracow
References
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Document Type
paper
Publication order reference
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YADDA identifier
bwmeta1.element.psjd-e555386f-f302-4140-8ff1-9bcd6188ef92
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