Kinetic studies for the production of biodiesel from jojoba oil and methanol in the presence of biocatalyst Novozyme 40013 (lipase from Candida antarctica) has been studied in the present research investigation along with optimization of reaction parameters. Optimum conditions identified for the present transesterification reaction are 5:1 molar ratios of alcohol to jojoba oil, 65 °C temperature with 5% concentration of catalyst for 7 hrs of reaction duration and 600 rpm of mixing intensity which contributed 95.12% conversion. Pseudo homogeneous first order kinetic model has been applied to fit the experimental data and results have been analysed on the basis of model data. Equilibrium constants (K) have been evaluated through this kinetic model using optimized reaction parameters. By analyzing the kinetic model, the frequency factor and the activation energy of the transesterification reaction have been determined. The properties of jojoba biodiesel and diesel fuel are in good agreement which encourages us to use it as an alternative fuel in future.