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2018 | 72 | 108–115
Article title

Komórki macierzyste rąbka rogówki w procesie regeneracji nabłonka rogówki – aspekt kliniczny i molekularny

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EN
Limbal epithelial stem cells in regeneration of corneal epithelium – clinical and molecular aspects
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PL EN
Abstracts
EN
Limbal epithelial stem cells (LESC) are located at the junction between the cornea and sclera. Their function is to reconstruc-ting and replace damaged or dysfunctional cells. In this paper information on the characteristics of LESC, describes the methods of their identification using surface molecular markers and also morphological characteristics are included. The methods of LESC cultivation in vitro as a potential source of stem cells for therapy are also presented. The issues of corneal disorders due to limbal stem cell deficiency (LSCD) caused by various factors were described and discussed as well as the treatment methods currently applied in medicine. The standard treatment of LSCD is based on pharmacotherapy and autologous transplantation of the limbus which is rich in stem cells. However, in the case of total stem cell deficiency (TLSCD) allogenic transplantation is necessary.
PL
Komórki macierzyste rąbka rogówki (limbal epithelial stem cells – LESC) zlokalizowane są na granicy rogówki i spojówki. Ich funkcja polega na odbudowie rogówki poprzez zastępowanie uszkodzonych lub niefunkcjonalnych komórek. W niniejszej pracy zawarto informacje dotyczące charakterystyki LESC, opisano sposoby ich identyfikacji z wykorzystaniem markerów powierzchniowych oraz cech morfologicznych. W pracy zawarto również informacje dotyczące hodowli komórek w warunkach in vitro jako potencjalnego źródła komórek wykorzystywanych w terapii oraz poruszono zagadnienia dysfunkcji LESC spowodowanych niedoborem komórek macierzystych wywołanych różnymi czynnikami, a także przedstawiono stosowane obecnie metody leczenia LSCD (limbal stem cell deficiency). Standardowe leczenie niedoboru komórek macierzystych rąbka rogówki polega na farmakoterapii oraz transplantacji autologicznego rąbka rogówki bogatego w komórki macierzyste. W przypadku całkowitego niedoboru LESC konieczne jest wykonanie przeszczepów allogenicznych.
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Year
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72
Pages
108–115
Physical description
Contributors
  • Zakład Biologii Molekularnej, Katedra Biologii Molekularnej, Wydział Farmaceutyczny z Oddziałem Medycyny Laboratoryjnej w Sosnowcu, Śląski Uniwersytet Medyczny w Katowicach, bartoszsikora90@gmail.com
  • Zakład Biologii Molekularnej, Katedra Biologii Molekularnej, Wydział Farmaceutyczny z Oddziałem Medycyny Laboratoryjnej w Sosnowcu, Śląski Uniwersytet Medyczny w Katowicach
author
  • Oddział Okulistyki Dorosłych, Uniwersyteckie Centrum Kliniczne im. Prof. K. Gibińskiego Śląskiego Uniwersytetu Medycznego w Katowicach, Klinika Okulistyki, Katedra Okulistyki, Wydział Lekarski w Katowicach, Śląski Uniwersytet Medyczny w Katowicach, Klinika Okulistyki Katedry Okulistyki, Wydział Lekarski w Katowicach, Śląski Uniwersytet Medyczny w Katowicach
  • Katedra i Zakład Biochemii, Wydział Lekarski w Katowicach, Śląski Uniwersytet Medyczny w Katowicach
  • Stowarzyszenie Śląska Poliklinika Weterynaryjna
  • Zakład Biologii Molekularnej, Katedra Biologii Molekularnej, Wydział Farmaceutyczny z Oddziałem Medycyny Laboratoryjnej w Sosnowcu, Śląski Uniwersytet Medyczny w Katowicach
  • Uniwersyteckie Centrum Medycyny Weterynaryjnej UJ-UR w Krakowie
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