Antibiotics Evaluation of Bacterial Isolates Associated with Imo State University Microbiology Laboratory and Sub-Offices

• Authors: Nwakaire Princewill , Nnalue Anthony Chinedu , Justin Ikechukwu Diaku , Ejinwa Confidence Godwin
• Languages of publication: EN

Abstracts

EN

An analysis was carried out to determine the antibiotics evaluation of bacterial isolates associated with Imo State University (IMSU) Microbiology laboratory and sub offices. Swab sticks used for streaking were inoculated unto a freshly prepared nutrient agar, MacConkey agar for bacteria. Identification of bacteria was performed by determining their gram staining properties, biochemical reactions and sugar tests indicating their presence. The microbial isolates obtained from this study were identified to belong to bacteria genus of Klebsiella sp., Escherichia coli, Bacillus sp., Streptococcus sp., Staphylococcus sp., Shigella sp., Micrococcus sp., and Pseudomonas sp. Klebsiella sp. had the highest percentage of occurrence (39.3%) while Pseudomonas sp had the least occurrence (1.8%). Bacteria isolates showed the highest percentage of susceptibility to Tarivid (OFX) 21(25%) followed by Augmentin (AU) 13(15.5%), Gentamycin (CN) 10(11.9%), Nalidixic (NA) 9(10.7%), Ceporex (CEP) 7(8.3%), Septrin (SXT) 4(4.8%), Ciprofloxacin (CPX) 3(3.6%) while the lowest percentage of susceptibility was to Streptomycin (S) 1(1.2%). The effectiveness of decontamination ranges from high-level sterilization to simple cleaning with soap and water. Levels of decontamination include Sterilization, Disinfection, Antisepsis, and Cleaning. It is recommended that ethylene oxide gas is used in Sterilization of the air before any analysis is carried out.

Keywords

EN

Antibiotic susceptibility; Bacterial isolates; Gram-negative bacteria; Gram-positive bacteria; IMSU Microbiology laboratory; Sterilization and decontamination

Discipline

• MICROBIOLOGY: MICROBIOLOGY

• Publisher: Scientific Publishing House „DARWIN”
• Journal: World News of Natural Sciences
• Year: 2025
• Volume: 58
• Pages: 318-328

Contributors

author

Nwakaire Princewill

• Department of Microbiology, Faculty of Biological Sciences, Imo State University, Owerri, Nigeria

author

Nnalue Anthony Chinedu

• CAS Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, Fujian 361021, China

author

Justin Ikechukwu Diaku

• Department of Chemistry, Faculty of Physical Sciences, Imo State University, Owerri, Nigeria

author

Ejinwa Confidence Godwin

• Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmacy, University of Uyo, Akwa Ibom State, Nigeria

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• Document Type: article