Układy liposom-polimer jako nośniki białek zakotwiczonych przez GPI

• Authors: Magdalena Łukasiewicz
• Languages of publication: PL

Abstracts

EN

This paper presents the development of novel liposomal nanocarriers which can be used in delivery of biologically-active substances, such as proteins. There is a great interest in liposomal carriers, which could be used for medical and pharmacological applications. However, because of low stability of liposomes, such applications are challenging. Certain external factors, such as pH, temperature or the presence of other substances in the environment may have a damaging effect on the lipid vesicles. Proteins are known to play an essential role in the functioning of living organisms. They have mechanical-structural functions, but they can also act as biocatalysts (enzymes), neurotransmitters, or are involved in the transport of small molecules and ions in tissue. The interest in GPI-linked proteins is related to their ability to incorporate in the lipid bilayer. They have a glycosylphosphatidylinositol anchor with two long alkyl chains. These chains can incorporate in the cell membrane, thus allowing their attachment to the outer surface of vesicles. Coating them with polyelectrolytes can increase the stability of liposomes. It was found that some polymeric films have the ability to stabilize the liposomal nanocarriers of proteins. That can be correlated with the value of the zeta potential of polymer which coats liposomes, as well as with the shape of a polymeric chain. The polymeric coating can be carefully chosen to ensure that the stabilizing effect will be associated with protein protection reflected in its high enzymatic activity.

Keywords

EN

Aktywność białka; Białka kotwiczące przez GPI; Liposomy; Liposomy a odpowiedź immunologiczna; Technologia liposomalna; Trwałość liposomów i metody ich stabilizacji; Układy liposomalne

Discipline

• BIOLOGY: BIOLOGY

• Publisher: Towarzystwo Doktorantów. Uniwersytet Jagielloński
• Journal: Zeszyty Naukowe Towarzystwa Doktorantów Uniwersytetu Jagiellońskiego. Nauki Ścisłe
• Year: 2012
• Issue: 5
• Pages: 57-75

Contributors

author

Magdalena Łukasiewicz

• Uniwersytet Jagielloński

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• Document Type: article