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2012 | 5 | 57-75
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Układy liposom-polimer jako nośniki białek zakotwiczonych przez GPI

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This paper presents the development of novel liposomal nanocarriers which can be used in delivery of biologically-active substances, such as proteins. There is a great interest in liposomal carriers, which could be used for medical and pharmacological applications. However, because of low stability of liposomes, such applications are challenging. Certain external factors, such as pH, temperature or the presence of other substances in the environment may have a damaging effect on the lipid vesicles. Proteins are known to play an essential role in the functioning of living organisms. They have mechanical-structural functions, but they can also act as biocatalysts (enzymes), neurotransmitters, or are involved in the transport of small molecules and ions in tissue. The interest in GPI-linked proteins is related to their ability to incorporate in the lipid bilayer. They have a glycosylphosphatidylinositol anchor with two long alkyl chains. These chains can incorporate in the cell membrane, thus allowing their attachment to the outer surface of vesicles. Coating them with polyelectrolytes can increase the stability of liposomes. It was found that some polymeric films have the ability to stabilize the liposomal nanocarriers of proteins. That can be correlated with the value of the zeta potential of polymer which coats liposomes, as well as with the shape of a polymeric chain. The polymeric coating can be carefully chosen to ensure that the stabilizing effect will be associated with protein protection reflected in its high enzymatic activity.
  • Uniwersytet Jagielloński
  • Angelini G., Boncompagni S., De Maria P., Fontana A., Gasbarri C., Siani G., Kinetic evolution of the effect of layer by layer deposition of polyelektrolytes on the stability of POPC liposomes, „Colloids and Surfaces A: Physicochemical and Engineering Aspects” 2008, nr 322, s. 234–238.
  • Atrooz O. M., Effects of Alkylresorcinolic Lipids Obtained from Acetonic Extract of Jordanian Wheat Grains on Liposome Properties, „International Journal of Biological Chemistry” 2011, nr 5, s. 314–321.
  • Bendas G., Krause A., Bakowsky U., Vogel J., Rothe U., Targetability of novel immunoliposomes prepared by a new antibody conjugation technique, „International Journal of Pharmaceutics” 1999, nr 181, s. 79–93.
  • Bolean M., Simão A. M. S., Favarin B. Z., Millán J. L., Ciancaglini P., Thermodynamic properties and characterization of proteoliposomes rich in microdomains carrying alkaline phosphatase, „Biophysical Chemistry” 2011, nr 158, s. 111–118.
  • Cho-Ngwa F., Mbua E. N., Nchamukong K. G., Titanji V. P. K., Detection, purification and characterisation of a secretory alkaline phosphatase from Onchocerca species, „Biotechnology Unit, Department of Biochemistry and Microbiology, Molecular and Biochemical Parasitology” 2007, s. 136–143.
  • Ciobanu M., Heurtault B., Ruhlmann C., Muller C. D., Frisch B., Layersome: Development and optimization of stable liposomes as drug delivery system, „International Journal of Pharmaceutics” 2007, nr 344, s. 154–157.
  • Decher G., Schlenoff J. B., Multilayer thin films, Wiley-VCH 2003.
  • . Eisenhaber B., Maurer-Stroh S., Novatchkova M., Schneider G., Eisenhaber F., Enzymes and auxiliary factors for GPI lipid anchor biosynthesis and post-translational transfer to proteins, „BioEssays” 2003, nr 25, s. 367–385.
  • Feng C., Zhang C., Shao X., Liu Q., Qian Y., Feng L., Chen J., Zha Y., Zhang Q., Jiang X., Enhancement of nose-to-brain delivery of basic fibroblast growth factor for improving rat memory impairments induced by co-injection of β-amyloid and ibotenic acid into the bilateral hippocampus, „International Journal of Pharmaceutics” 2012, nr 423, s. 226–234.
  • Feng S.-S., Ruan G., Li Q.-T., Fabrication and characterizations of a novel drug delivery device liposomes-in-microsphere (LIM), „Biomaterials” 2004, nr 25, s. 5181–5189.
  • Ferguson M. A. The structure, biosynthesis and functions of glycosylphosphatidylinositol anchors, and the contributions of trypanosome research, „Journal of Cell Science” 1999, nr 112, s. 2799–2809.
  • Ferguson M. A., Homans S. W., Dwek R. A., Rademacher T. W., Glycosyl-phosphatidylinositol moiety that anchors Trypanosoma brucei variant surface glycoprotein to the membrane, „Science” 1988, nr 239, s. 753–759
  • Ferguson M. A., Low M. G., Cross G. A., Glycosylsn-1,2-dimyristylphosphatidylinositol is covalently linked to Trypanosoma brucei variansurface glycoprotein, „The Journal of Biological Chemistry” 1985, nr 260, s. 14547–14555.
  • Fukui Y., Fujimoto K., The Preparation of Sugar Polymer-Coated Nanocapsules by the Layer-by-Layer Deposition on the Liposome, „Langmuir” 2009, nr 17, s. 10020–10025.
  • GPI-anchored biomoleculessan oVerView, in GPIanchored membrane proteins and carbohydrates, eds. D. C. Hoessli, S. Ilangumaran, 1999, s 1–14.
  • Helenius A., Simons K., Solubilization of membranes by detergents, “Biochimica et Biophysica Acta” 1975, nr 415 (1), s. 29–79.
  • Homans S. W., Ferguson M. A., Dwek R. A., Rademacher T. W., Anand R., Williams A. F., Complete structure of the glycosyl phosphatidylinositol membrane anchor of rat brain Thy-1 glycoprotein, „Nature” 1988, nr 333, s. 269–272.
  • Immordino M. L., Dosio F., Cattel L., Stealth liposomes: review of the basic science, rationale, and clinical applications, existing and potential, „International Journal of Nanomedicine” 2006, s. 297–315
  • Iqbal J., An enzyme immobilized micorassay in capillary electrophoresis for characterization and inhibition studies of alkaline phosphatases, „Department of Pharmaceutical Sciences, Analytical Biochemistry” 2011, nr 414, s. 226–231
  • . Kopczyński P., Możliwości zastosowania wybranych markerów obrotu kostnego w stomatologii, „Nowiny Lekarskie” 2004, nr 73, s. 318–320.
  • Lasic D., Ceh B., Stealth Liposomes: From Theory To Product, „Advanced Drug Delivery Reviews” 1994, nr 24s. 165–177
  • Machluf M., Regev O., Peled Y., Kost J., Cohen S., Characterization of microencapsulated liposome system for the controlled delivery of liposome- associated macromoleculs, „Journal of Controlled Release” 1996, nr 43, s. 35–45.
  • Marquez L., Mauad de Abrau F. A., Ferreira C., Alves G., Miziara M., Alves J., Enhanced bone healing of rat tooth sockets after administration of epidermal growth factor (EGF) carried by liposome, „Injury” 2012, w druku.
  • Murray E. W., Robbins S. M., Antibody Cross-linking of the Glycosylphosphatidylinositollinked Protein CD59 on Hematopoietic Cells Induces Signaling Pathways Resembling Activation by Complement, „The Journal of Biological Chemistry” 1998, nr 273 s. 25279– 25284.
  • Nakanishi T., Hayashi A., Kunisawa J., Tsutsumi Y., Tanaka K., Yashiro Ohtani Y., Nakanishi M., Fujiwara H., Hamaoka T., Mayumi T., Fusogenic liposomes afficiently deliver exogenous antigen through the cytoplasm into the MHC class I processing pathway, „European Journal of Immunology” 2000, nr 30, s. 1740–1747.
  • Nishioka T., Tomatsu S., Gutierrez M. A., Miyamoto K. I., Trandafirescu G. G., Lopez P. L. C. et al., Enhancement of drug delivery to bone: characterization of human tissuenonspecific alkaline phosphatase tagged with an acidic oligopeptide, „Molecular Genetics and Metabolism” 2006, nr 88, s. 244–255.
  • Nosjean, O., Briolay A., Roux, B., Mammalian GPI proteins: sorting, membrane residence and functions, „Biochimica et Biophysica Acta” 1997, nr 1331, s. 153–186
  • Orimo H., The Mechanism of Mineralization and the Role of Alkaline Phosphatase in heath and disease, „Division of Medical and Biological Chemistry, Graduate School of Medicine, Journal of Nippon Medical School” 2010, s. 4–12.
  • Osathanon T., Giachelli C. M., Somerman M. J., Immobilization of alkaline phosphatase on microporous nanofibrous fibrin scaffolds for bone engineering, „Biomaterials” 2009, s. 4513–4521.
  • Petty H., Characterization of Cellular Proteins, „Current Protocols in Cell Biology” 1998, s. 5.1.1–5.1.10.
  • . Poelstra K., Bakker W. W., Klok P. A., Hardonk M. J., Meijer D. K., A physiologic function for alkaline phosphatase: endotoxin detoxification, „Laboratory Investigation” 1997, nr 76, s. 319–327.
  • Ronzon F., Morandat S., Roux B., Bortolato M., Insertion of a Glycosylphosphatidylinositol-Anchored Enzyme into Liposomes, „The Journal of Membrane Biology” 2004, nr 197, s. 169–177.
  • . SooJee S., Culver L., Li Y., Douglas E. P., Gower L. B., Biomimetic mineralization of collagen via an enzyme-aided PILP process, „Journal of Crystal Growth” 2010, nr 312, s. 1249–1256.
  • Stebelska K., Wyrozumska P., Grzybek M., Sikorski A. F., Characterization and medical applications of liposome constructions, „Advances in Clinical and Experimental Medicine” 2002, nr 11 (2), s. 229–242.
  • . Suzuki K., Okumura Y., GPI-Linked Proteins Do Not Transfer Spontaneously from Erythrocytes to Liposomes. New Aspects of Reorganization of the Cell Membrane, „Biochemistry” 2000, nr 39, s. 9477–9485
  • Takeuchi H., Yamamoto H., Toyoda T., Toyobuku H., Hino T., Kawashima Y., Physical stability of size controlled small unilameller liposomes coated with a modified polyvinyl alcohol, „International Journal of Pharmaceutics” 1998, nr 164, s. 103–111.
  • Tiede A., Bastisch I., Schubert J., Orlean P., Schmidt R. E., Biosynthesis of glycosylphosphatidylinositols in mammals and unicellular microbes, „The Journal of Biological Chemistry” 1999, nr 380, s. 503–523.
  • Xiang Q., Xiao J., Zhang H., Zhang X., Lu M., Zhang H., Su Z., Zhao W., Lin C., Huang Y., Li X., Preparation and characterisation of bFGF-encapsulated liposomes and evaluation of wound-healing activities in the rat, „Burns” 2011, nr 37, s. 886–895.
  • Xiaomei M., Zhensheng Z., Preparation and properties of poly(vinyl alcohol)-stabilized liposomes, „International Journal of Pharmaceutics” 2006, nr 318, s. 55–61.
  • Zimecki M., Artym J., Effect of methotrexate on the immune response in selected experimental models, „Postępy Higieny i Medycyny Doświadczalnej” 2004, nr 58, s. 226–235.
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