Preferences help
enabled [disable] Abstract
Number of results
2020 | 145 | 286-297
Article title

The effect of phosphate addition with different concentration on the capability of Nannochloropsis oculata as a bioremediation agent of medium heavy metal (Cd2+)

Title variants
Languages of publication
Nannochloropsis oculata is a unicellular marine microalgae that has a role as an important food source and additive for marine organisms. Nannochloropsis oculata contains high levels of carbohydrate, protein, and chlorophyll so it has the potential as a bioremediation agent. In its growth, it needs an important component of nutrients as a growth medium, one of which is a source of phosphate. Phosphate itself acts as a constituent of protein compounds to bind metal ions in cells. This research aims to determine the effect of the addition of KH2PO4 as a phosphate source on the growth of Nannochloropsis oculata as a bioremediation agent to reduce levels of heavy metal pollution (Cd2+). This research was conducted from June to August 2019 in the Laboratory of Microbiology and Molecular Biotechnology of FIP-UNIDA, Bogor, West Java. Nannochloropsis oculata microalgae were obtained from UGO Plankton Shop, Purwodadi, Central Java. The research method used is a Completely Randomized Design (CRD) with 4 treatments and two replications. The Cd2+ concentration used is 2 ppm in this research. The addition of KH2PO4 as a phosphate source is 0 ppm (control), 3, 4, and 5 ppm. The results of this research showed that the highest growth peak of Nannochloropsis oculata was achieved at 4 ppm treatment on the 9th day with cell density reaching 17.8 × 106 cells/mL, while the highest absorption of cadmium (Cd2+) was found in the 4 ppm phosphate treatment with the absorption efficiency reaching 39.23%.
Physical description
  • Faculty of Fisheries and Marine Science, Universitas Padjadjaran, Sumedang, Indonesia
  • Faculty of Fisheries and Marine Science, Universitas Padjadjaran, Sumedang, Indonesia
  • Faculty of Fisheries and Marine Science, Universitas Padjadjaran, Sumedang, Indonesia
  • [1] Bergman SA. Ketamine: review of its pharmacology and its use in pediatric anesthesia. Anesth Prog. 46(1) (1999) 10-20
  • [2] Rizal A & Nurruhwati I. New Methodological Approaches for Change in Traditional Sectors: The Case of the West Java Fisheries Socio Economic System. World News of Natural Sciences 22 (2019) 41-51
  • [3] Rizal A. Reformulation of Regional Development Strategy To Strengthen Marine Sector in West Java, Indonesia. World Scientific News, 107 (2018) 207-215
  • [4] Rizal A, Science and policy in the coastal zone management. World News of Natural Sciences 21 (2018) 1-8
  • [5] Mosich TD, Bunn SE, Davies PM. The Relative Importance of Shading and Nutrients on Algal Production. Subtrofical Streams 46 (2001) 1269-1278
  • [6] Putri FDM, Endang W, Christiani. The Relationship of Comparison of Total Nitrogen and Total Phosphorus with the Abundance of Chrysophyta in the Water of Panglima Besar Reservoir, Soedirman, Banjarnegara. Scripta Biologica 1 (1) (2014) 96-101
  • [7] Naraghi, Z. G., Yaghmaei, S., Mardanpour, M. M., & Hasany, M., 2015. Produced water treatment with simultaneous bioenergy production using novel bioelectrochemical systems. Electrochim Acta 180, 535-544
  • [8] Cai, T., Ge, X., Park, S. Y., & Li, Y., 2013. Comparison of Synechocystis sp. PCC6803 and Nannochloropsis salina for lipid production using artificial seawater and nutrients from anaerobic digestion effluent. Bioresour Technol, 144, 255-260
  • [9] Hidaka, T., Inoue, K., Suzuki, Y., & Tsumori, J., Growth and anaerobic digestion characteristics of microalgae cultivated using various types of sewage. Bioresour Technol, 170 (2014) 83-89
  • [10] Hala Yusafir, Taba Paulina, Asmawati, Ruth Langan Febryanti. Potential of Marine Microalgae Nannochloropsis sp. As Biosorbent Ni2+, Cu2+, and Zn2+ in Medium Conwy Contaminated with Three Metal Mixture. Chemistry Progress. 7(2) (2014) 51-57
  • [11] Kamoru AS. Towards Sustainalbe development of microalgael biosorption for treating effluents containing heavy metals. Biofuel Research Journal (22) (2019) 948-961.
  • [12] [GOI] Government of Indonesia. Regulation Number 85 of 1999. Amendment to the Government of Indonesia Regulation Number 18 of 1999 concerning Management of Hazardous and Toxic Wastes. October 7, 1999. Jakarta.
  • [13] [GOI] Government of the Indonesia. Number 82 Year 2001. Regarding Water Quality Management and Water Pollution Control.
  • [14] Arriada, ADA., & Abreu, PC. Nannochloropsis oculata growth in produced water: an alternative for massive microalgae biomass production. Brazil J Petrol Gas, 8(3) (2014) 94-102
  • [15] Converti A., Casazza AA., Ortiz, EY., Perego, P., & Del Borghi, M. Effect of temperature and nitrogen concentration on the growth and lipid content of Nannochloropsis oculata and Chlorella vulgaris for biodiesel production. Chem Eng Processi: Proc Intensific 48(6) (2009) 1146-1151
  • [16] Jiang, L., Luo, S., Fan, X., Yang, Z., & Guo, R., Biomass and lipid production of marine microalgae using municipal wastewater and high concentration of CO2. Appli Energ, 88(10) (2011) 3336-3341
  • [17] Aransiola, E. F., Ojumu, T. V., Oyekola, O. O., Madzimbamuto, T. F., & IkhuOmoregbe, DIO. A review of current technology for biodiesel production: State of the art. Bioma Bioener, 61 (2014) 276-297
  • [18] Hamawand, I., Yusaf, T., & Hamawand, S., 2014. Growing algae using water from coal seam gas industry and harvesting using an innovative technique: A review and a potential. Fuel, 117, 422-430
  • [19] Baccella S, Cerichelli G, Chiarini M, Ercole C, Fantauzzi E, Lepidi A, Toro L, Veglio F.,. Biological treatment of alkaline industrial waste waters. Proc Biochemis 35(6) (2000) 595-602
  • [20] Mendes, LBB., & Vermelho, AB. Allelopathy as a potential strategy to improve microalgae cultivation. Biotechnol Biofuels, 6(1) (2013) 152-158.
  • [21] Graneli E., Weberg M., & Salomon PS. Harmful algal blooms of allelopathic microalgae species: the role of eutrophication. Harmf alg, 8(1) (2008) 94-102
  • [22] Duraisamy, RT., Beni, AH., & Henni, A., 2013. State of the art treatment of produced water. In Water Treat. InTech.
  • [23] Roleda, MY., Slocombe, Stephen P., Leakey, Raymond JG., Day, John G., Bell, Elanor M. & Stanley, Michele S. Effects of temperature and nutrient 23 regimes on biomass and lipid production by six oleaginous microalgae in batch culture employing a two-phase cultivation strategy. Bioresou Tech 129 (2013) 439-449
  • [24] El-Kassas HY. & Khairy, HM. A trial for biological control of a pathogenic fungus (Fusarium solani) by some marine microorganisms. Am-Euras J Agric Environ Sci 5 (2009) 434-440
  • [25] Converti, Attilio, Casazza, Alessandro A., Ortiz, Erika Y., Perego, Patrizia, & Del Borghi, Marco. Effect of temperature and nitrogen concentration on the growth and lipid content of Nannochloropsis oculata and Chlorella vulgaris for biodiesel production. Chem Eng Processi: Proc Intensific 48(6) (2009) 1146-1151
  • [26] Griffiths MJ. & Harrison ST. Lipid productivity as a key characteristic for choosing algae species for biodiesel production. J Appli Phyco 21(5) (2009) 493-507
  • [27] Lefebvre O. Moletta R. Treatment of organic pollution in industrial saline wastewater: a literature review. Water research, 40(20) (2006) 3671-3682.
  • [28] Rizal A, Izza MA, Heti H, Lantun P. Distribution and Condition of Coral Reefs in the Waters of Biawak Island, Indramayu Regency, West Java, Indonesia. World Scientific News, 144 (2020) 141-157
  • [29] Rizal A, Andriani Y, Kusumartono FXH, A Strategic Environmental Assessment for Southern Coastal of West Java Province, Indonesia. World Scientific News, 137 (2019) 188-209
  • [30] Rizal A, Nora A, Pringgo Kdyp, Rega P, Aulia A. Molecular diversity of the bacterial community associated with Acropora digitifera (Dana, 1846) corals on Rancabuaya coastline, Garut District, Indonesia. World Scientific News, 144 (2020) 384-396
  • [31] Dionisius Wahyu, Dwi Hindarti, Rega Permana, Cadmium Toxicity Towards Marine Diatom Thalassiosira sp. and its Alteration on Chlorophyll-a and Carotenoid Content. World News of Natural Sciences 31 (2020) 48-57
  • [32] Hassan Mohamed Ihmed Magelsir. Heavy metal toxicity - metabolism, absorption, distribution, excretion and mechanism of toxicity for each of the metals. World News of Natural Sciences 4 (2016) 20-32
Document Type
Publication order reference
YADDA identifier
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.