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2016 | 19 | 2 | 32-39
Article title

The effect of citrus flavanones on the redox homeostasis in cells exposed to oxidative stress – studies in vitro

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Title variants
PL
Wpływ flawanonów cytrusowych na homeostazę redoks komórek narażonych na stres oksydacyjny – badania in vitro
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EN PL
Abstracts
EN
Introduction: Flavanones (an important group of antioxidants in citrus fruits) are beneficial for health, which is connected with their anti-inflammatory, anti-atherogenic and anti-carcinogenic properties. The present study was undertaken to investigate whether – and in what way – the presence of flavanones influences the redox homeostasis of fibroblasts and alleviates the effects of oxidative stress. Material and methods: The study was conducted on murine fibroblast cell cultures with the addition of flavanones (hesperidin, hesperetin, naringin, naringenin), exposed to oxidative stress (Fe/Asc). In cell homogenates, the activity of superoxide dismutase (SOD) and glutathione peroxidase (GPx) was measured; in the medium, the concentration of nitric oxide was measured. Results and conclusion: Our results demonstrate that the addition of naringenin, hesperetin, naringin and hesperidin has a protective effect on cells subjected to oxidative stress The changes observed are particularly visible in the case of aglycone forms of both compounds. Despite the protective properties against oxidative stress which flavanones display, we determined distrubances in redox homeostasis in comparison to the control culture.
PL
Wstęp: Flawanony, będące głównymi związkami bioaktywnymi cytrusów, wykazują działanie prozdrowotne, co wynika z ich właściwości przeciwzapalnych, przeciwmiażdżycowych i przeciwnowotworowych. Celem badania była ocena wpływu flawanonów na homeostazę redoks fibroblastów oraz czy ich obecność łagodzi skutki stresu oksydacyjnego. Materiały i metody: Badanie przeprowadzono na hodowlach mysich fibroblastów z dodatkiem flawanonów (hesperydyna, hesperetyna, naringina, naringenina) poddanych stresowi oksydacyjnemu (Fe/Asc). W homogenatach komórkowych oznaczono aktywność dysmutazy ponadtlenkowej (SOD) i peroksydazy glutationowej (GPx), a bezpośrednio w pożywce stężenie tlenku azotu. Wyniki i wnioski: Wyniki naszej pracy wskazują, że dodatek naringeniny, hesperetyny, naringiny i hesperydyny działa ochronnie na komórki poddane stresowi oksydacyjnemu. Pomimo stwierdzonego ochronnego działania flawanonów zaobserwowaliśmy również zaburzenie homeostazy redoks w odniesieniu do hodowli kontrolnej.
References
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Document Type
review
Publication order reference
YADDA identifier
bwmeta1.element.psjd-d0a5b4eb-40c5-4d46-a1c3-0d7d3b037147
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