Preferences help
enabled [disable] Abstract
Number of results
2019 | 115 | 128-137
Article title

Technique for retardation of leaf senescence of six ericoid wild plant taxa using ascorbic acid

Title variants
Languages of publication
Efficacy of a growth promoter ascorbic acid (Vitamin C) on senescence deferral action was analysed using leaf discs of six wild ericoid plant taxa. Changes of some biochemical parameters like chlorophyll, protein, soluble and insoluble carbohydrates, RNA and DNA as well as activity of catalase enzyme were analysed as reliable senescence indices during detached leaf senescence of these six species under dark condition. With the progress of ageing duration from zero to 144 hours the levels of chlorophyll and proteins in leaf discs gradually declined in both control and ascorbic acid treated samples. However, in the chemical / Vitamin-C treated samples the rate of decline was found to be much slower. Concomitantly the levels of insoluble carbohydrate, RNA and DNA started declining right from 48, 96 and 144 hours of observation period both in treated and untreated samples. And here also ascorbic acid arrested the rapid rate of reduction of the levels of the biochemical parameters. On the other hand, soluble carbohydrate level started increasing irrespective of the treatments during the whole observation periods. However, the magnitude of increase was found to be low in case of the leaf samples which experienced treatment with ascorbic acid. The activity of the enzyme catalase was found to decrease progressively during the four observation periods (0, 48, 96 and 144 hours) regardless of the treatments. Ascorbic acid partially averted the rapid fall of the enzyme activity during the ageing periods. Ascorbic acid, a nonconventional senescence deferred thus seems to be a potent senescence deferral phytohormone /chemical / vitamin at least in case of six ericoid wild plant taxa.
Physical description
  • Angiosperm Taxonomy & Ecology Lab, Botany Department, Maulana Azad College, University of Calcutta, Kolkata - 700013, India
  • Plant Physiology and Biochemistry Lab, Department of Botany, Saldiha College, Bankura University, Saldiha - 722173, India
  • Plant Physiology and Biochemistry Lab, Department of Botany, University of Burdwan, Burdwan - 713104, India
  • Post-Graduate Department of Botany, Sabang Sajanikanta Mahavidyalaya, Vidyasagar University, Medinipur, 721102, West Bengal, India
  • [1] Leopold AC and Kriedemann PE (1975). Plant growth and development, TataMcGraw Hill Publ. Co., New Delhi, India.
  • [2] Pati CK (2007). Seed invigouration, plant potentiation and yield augmentation of two promising pulse pulse crops (Lathyrus sativus L. and Vigna mungo (L.) Hepper by chemical manipulation. Ph.D. thesis, Vidyasagar University, West Bengal, India.
  • [3] Sabater B (1984). Hormonal regulation of senescence. In: Hormonal regulation of plant growth and development, S.S. Purohit (Ed.), Agro Botanical Publ. India, 1: 169-217.
  • [4] Richmond AE and Lang A (1957). Effect of kinetin on protein content and survival of detached Xanthium leaves. Science 125: 650-651.
  • [5] Van Staden J, Cook EL and Nooden LD (1988). Cytokinins and Senescence. In: Senes-cence and Ageing Plants, L.D. Nooden and A.C. Leopold, (Ed.), Academic Press, New York, pp. 281-328.
  • [6] Biswas AK and Ghosh AK (1999). Regulation of Senescence in Various Plants. Emkay Pub. New Delhi, India.
  • [7] Arnon DI (1949). Copper enzymes in isolated chloroplasts. Polyphenol oxidase in Beta vulgaris. Plant Physiol. 24: 1-15.
  • [8] Kar M and Mishra D (1976). Catalase, peroxidase, polyphenol-oxidase activities during riceleaf senescence. Plant Physiol. 57: 315-319.
  • [9] Lowry OH, Rosebrough NJ, Farr AL and Randall RJ (1951). Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265-275.
  • [10] McCready RM, Guggloz J, Silviera V and Owens HS (1950). Determination of starch and amylase in vegetables. Analyt. Chem. 22: 1156-1158.
  • [11] Cherry JH (1962). Nucleic acid determination in storage tissue of higher plants. Plant Physiol. 37: 670-678.
  • [12] Markham R (1955). In: Modern Methods of Plant Analysis. Vol. IV. K Peach and MV Tracey, (Ed.) Springer Verlag, Berlin, pp. 246-304.
  • [13] Choudhuri MA and Chattarjee SK (1970). Seasonal changes in the level of some cellular components in the abscission zone of Coleus leaves of different ages. Ann. Bot. 34: 275-287.
  • [14] Snell FD and Snell CT (1971). In: Colorimetric Methods of Analysis. Vol. 4. Van Nostrand Reinhold Co., New York, USA.
  • [15] Biswas AK and Choudhuri MA (1978). Differential behaviour of the flag leaf of intact rice plant during ageing. Biochem. Physiol. Pflanzen. 173: 220-228.
  • [16] Pati CK and Bhattacharjee A (2003). Chemical induced regulation of detached leaf senescence of a few ecologically diversed plants. Environment and Ecology 21: 3, 607-611.
  • [17] Woolhouse HO (1967). The nature of senescence of plants. Symp. Soc. Exp. Biol. 21: 179-214.
  • [18] Leopold AC (1980). Ageing and senescence in plant development. Pages 1-12 in K.V. Thimann, editor, Senescence in plants. CRC Press, Florida, USA.
  • [19] Beevers L (1976). Plant Biochemistry (J Bonner, J.E. Verner eds.), Academic Press, New York, pp. 771-794.
  • [20] Maity S, Banerjee G, Roy M, Pal C, Pal B, Chakrabarty D and Bhattacharjee A (2000). Chemical induced prolongation of seed viability and stress tolerance capacity of mungbean seedlings. Seed Sci. & Technol. 28: 155-162.
  • [21] Fridovich I (1976). Oxygen radical, hydrogen peroxide and oxygen toxicity. In : Free radicals in biology, Vol. 1, WA Pryer (Ed.), Academic Press, New York, pp. 239-277.
  • [22] Bhattacharjee A and Choudhuri MA (1986). Chemical manipulation of seed longevity and stress tolerance capacity of seedlings of Corchorus capsularis and C. olitorius. J. Plant Physiol, 125: 391-400
  • [23] Bhattacharjee A (2001). Influence of a growth retardant and volatile oil on enhancement of storage longevity of two low vigour seed species. Nat. Bot. Soc. 55: 55-61.
Document Type
Publication order reference
YADDA identifier
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.