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2019 | 25 | 141-154
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An Improved Micropropagation Protocol for Manga Bamboo - Pseudoxytenanthera stocksii (Munro) T.Q. Nguyen

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The present report illustrates an improved and reproducible micropropagation system for economically valuable bamboo species Pseudoxytenanthera stocksii using nodal segments. Direct organogenesis from nodal segments was accomplished by culturing on Murashige and Skoog (MS) medium supplemented with 6.0 mg/L 6-benzylaminopurine (BAP). On this medium combination 9.0±0.25 shoots per explant were induced. Shoot multiplication was subjective to the combination of auxins and cytokinins concentrations used and number of repeated transfer of mother explants or subculturing of in vitro regenerated shoot clumps on fresh medium. Maximum 41.9±1.00 shoots were observed on MS medium supplemented with 4.0 mg/L of BAP and 0.25 mg/L of naphthalene acetic acid (NAA). Half strength MS medium containing various concentrations of auxins was used for in vitro root formation. Successful rooting with maximum response was achieved on half strength MS medium + 50% sucrose and 1.0 mg/L IBA (24.3±0.27 roots). The rooted plantlets were planted the soilrite and hardened in the greenhouse for 6-8 weeks. Completely acclimatized plantlets exhibited 96% survivability. The present propagation protocol could satisfy the demand of P. stocksii and could be explored for the commercial propagation of this valuable manga bamboo.
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  • Tissue Culture Lab, Silviculture and Forest Utilization, Research Range, Rajpipla, Dist. Narmada, Gujarat, India
  • Tissue Culture Lab, Silviculture and Forest Utilization, Research Range, Rajpipla, Dist. Narmada, Gujarat, India
  • Tissue Culture Lab, Silviculture and Forest Utilization, Research Range, Rajpipla, Dist. Narmada, Gujarat, India
  • Siddha Medicinal Plants Garden, Central Council for Research in Siddha, Mettur, TN, India
  • Biotechnology Laboratory, K. M. Centre for P. G. Studies, Puducherry - 605 008, India
  • [1] P. Venkatachalam, K. Kalaiarasi, S. Sreeramanan, Influence of plant growth regulators (PGRs) and various additives on in vitro plant propagation of Bambusa arundinacea (Retz.) Wild: A recalcitrant bamboo species. Journal of Genetic Engineering and Biotechnology 16 (2015) 193-200
  • [2] FAO (Food and Agriculture Organization of the United Nations), Global forest resources assessment, 2000: Main report, Rome, FAO. (2001) 479
  • [3] Y. Qing, D. Zhu-Biao, ., Zheng-liang, H. Kai-Hong, S. Qi-Xiang, P. Zhen-Hua, Bamboo resources, utilization and ex-situ conservation in Xishuangbanna, South-eastern China. Journal of Forestry Research 19 (2008) 79–83
  • [4] K.K. Seethalakshmi, M.S.M. Kumar, Bamboos of India: A compendium. Bamboo Information Centre: India, KFRI, Peechi and International Network for Bamboo and Rattan, New Delhi, India, 1998
  • [5] R. Mahapatra, S. Shrivastava, Bamboo rising. Down to Earth, 16-31 (2013) 25-32
  • [6] Adarsh Kumar, Mass production of field planting stock of Dendrocalamus strictus through macroproliferation- a technology. Indian Forester 117 (1991) 1046-4052
  • [7] R.L. Banik, Review of conventional propagation research in bamboos and future strategy. Constraints to Production of Bamboo and Rattan. International Network for Bamboo and Rattan (1994) 115-142
  • [8] P. Bisht, M. Pant, A. Kant, In vitro propagation of Gigantochloa atroviolacea Widjaja through nodal explants. Journal of Americal Science 6 (2010) 1019-1025
  • [9] W.S. Devi, L. Bengyella, G.J. Sharma, In vitro seed germination and micropropagation of edible bamboo Dendrocalamus giganteus Munro using seeds. Biotechnology 11 (2012) 74-80
  • [10] S.D. Waikhom, B. Louis, An effective protocol for micropropagation of edible bamboo species (Bambusa tulda and Melocanna baccifera) through nodal culture. The Scientific World Journal (2014)
  • [11] H. Saini, I.D. Arya, S. Arya, R. Sharma, In vitro micropropagation of himalayan weeping bamboo, Drepanostachyum falcatum. American Journal of Plant Sciences 07 (2016) 1317-1324
  • [12] R.I. Raju, S.K. Roy, Mass propagation of Bambusa bambos (L.) Voss through in vitro culture. Jahangirnagar University Journal of Biological Science 5 (2016) 15-26
  • [13] I. Ansari, S. Gupta, S. Soni, A. Baig, In-vitro regeneration of Bambusa balcooa (Bamboo) through nodal segments. International Journal of Current Microbiology and Applied Sciences 6 (2017) 4901-4905
  • [14] B. S. Rajput, M. D. Jani, M. R. Gujjar, M. S. Shekhawat, Effective and large scale in vitro propagation of Dendrocalamus strictus (Roxb.) Nees using nodal segments as explants. World Scientific News 130 (2019) 238-249
  • [15] R. M. Singhal, G. Gangopadhyay, Bamboos in India, Publication Division, ICFRE, Dehradun (1999) 147
  • [16] A.J. Nath, A.K. Das, Bamboo resources in the home gardens of Assam: A case study from Barak Valley. Journal of Tropical Agriculture 46 (2008) 46-49
  • [17] S. Viswanath, G. Joshi, P.V. Somashekar, A.D. Rane, S. Chandramouli, S.C. Joshi, Dendrocalamus stocksii (Munro.): A Potential Multipurpose Bamboo Species for Peninsular India. Institute of Wood Science and Technology (Indian Council for Forestry Research & Education) Bangalore, 2013
  • [18] A. Rane, Morphological, Physiological and genetic variations in Dendrocalamus stocksii (Munro) along the Central Western Ghats. Ph.D thesis, FRI Deemed University, Dehra Dun, Uttaranchal, 2015
  • [19] AOAC-Association of Official Analytical Chemists. Official Methods of Analysis of AOAC International, 16th ed., 4th rev. AOAC International, Gaithersburg, MD. 1998
  • [20] AOAC-Association of Official Analytical Chemists. Official Methods of Analysis.18th ed. Assoc. Anal.Chem., Arlington. VA. 2005
  • [21] A.D. Rane, S. Chandramouli, S. Viswanath, Can Dendrocalamus stocksii (munro.) be the ideal multipurpose bamboo species for domestication in Peninsular India?. Journal of Bamboo and Rattan 15 (2016) 23-32
  • [22] P. Rawat, S.S. Narkhede, A.D. Rane, V.M. Mhaiske, V.V. Dalvi, Allelopathic effect of Dendrocalamus stocksii (Munri.) on growth and yield of ground nut (Arachis hypogaea). Journal of Applied and Natural Science 10 (2018) 881-885
  • [23] T. Murashige, F. Skoog, A Revised Medium for Rapid Growth and Bioassay with Tobacco Tissue Cultures. Physiology Plant 15 (1962) 473-497
  • [24] V.M. Jiménez, E. Guevara, Micropropagation of Bamboo Species Through Axillary Shoot Proliferation. In: Jain S.M., Häggman H. (eds) Protocols for Micropropagation of Woody Trees and Fruits. Springer, Dordrecht, 2007
  • [25] R. Thakur, A. Sood, An efficient method for explants sterilization for reduced contamination. Plant Cell, Tissue and Organ Culture 3 (2006) 369-371
  • [26] S.S. Ray, M.N. Ali, S. Mukherjee, G. Chatterjee, M. Banerjee, Elimination and molecular identification of endophytic bacterial contaminants during in vitro propagation of Bambusa balcooa. World Journal of Microbiology and Biotechnology 33 (2017)
  • [27] A.H. Ali, C. Nirmala, T. Badal, M.L.Sharma, In-vitro organogenesis and simultaneous formation of shoots and roots from callus in Dendrocalamus asper. In: 8th World Bamboo Congress, Thailand (2009) 31-40
  • [28] S. Arya, A. Kant, D. Sharma, I.D. Arya, Micropropagation of two economically important bamboos: Drepanostachyum falcatum (Nees) Keng and Bambusa balcooa Roxb. Indian Forest 134 (2008) 1211–1221
  • [29] R. K. Agnihotri, S. K. Nandi, In vitro Shoot Cut: A High Frequency Multiplication and Rooting Method in the Bamboo Dendrocalamus hamiltonii. Biotechnology 8 (2009) 259-263
  • [30] P. Bisht, M. Pant, A. Kant, In vitro propagation of Gigantochloa atroviolaceae Widjaja through nodal explants. Journal of American Science 6 (2010) 1019-1025
  • [31] A. Jha, S. Das, B. Kumar, Micropropagation of Dendrocalamus hamiltonii through nodal explants. Global Journal of Bio-Science and BioTechnology 2 (2013) 580-582
  • [32] L.C. Huang, B.L. Huang, W.L. Chen, Tissue culture investigations of bamboo IV Organogenesis leading to adventitious shoots and plants excised from shoot apices. Environmental and Experimental Botany 29 (1989) 307-315
  • [33] L.C. Huang, B.L. Huang, Loss of the species distinguishing trait among regenerated Bambusa ventricosa McClure plants. Plant Cell, Tissue and Organ Culture 42 (1995) 109-111
  • [34] Bag N, Chandra S, Palni LMS, Nandi S, Micropropagation of Dev-ringal [Thamnocalamus spathiflorus (Trin.) Munro] - A temperate bamboo, and comparison between in vitro propagated plants and seedlings. Plant Science 156 (2000) 125-135
  • [35] S.R. Singh, S. Dalal, R. Singh, A. Dhawan, R.K. Kalia, Micropropagation of Dendrocalamus asper {Schult. & Schult. F.} Backer ex k. Heyne): An exotic edible bamboo. Journal of Plant Biochemistry and Biotechnology 21 (2012) 220-228
  • [36] M. Sandhu, S.H. Wani, V.M. Jimenez, In vitro propagation of bamboo species through axillary shoot proliferation: a review. Plant Cell Tissue Organ Culture 132 (2018) 27-53
  • [37] T.S.R. Sanjaya, V.R. Rai, Micropropagation of Pseudoxytenanthera stocksii Munro. In Vitro Cellular & Developmental Biology - Plant 41 (2005) 333-337
  • [38] S. Arya, R. Satsangi, I.D. Arya, Rapid mass multiplication of edible bamboo Dendrocalamus asper. Journal of Sustainable Forestry 4 (2002) 103-109
  • [39] T.S. Rathore, U. Kabade, M.R. Jagadish, P.V. Somashekar, S. Viswanath, Micropropagation and evaluation of growth performance of the selected industrially important bamboo species in southern India. In: 8th world bamboo congress, Thailand, (2009) 41–55
  • [40] P. Sharma, K.P. Sarma, In vitro propagation of Bambusa balcooa for a better environment. In: International conferences on advances in biotechnology and pharmaceutical sciences (ICABPS’11). Bangkok, (2011) 248–252
  • [41] E.E.E. Diab, S.E. Mohamed, In vitro morphogenesis and plant regeneration of bamboos (Oxytenanthera abyssinica A. Rich. Munro). International Journal of Sustainable Crop Production 3 (2008) 72-79
  • [42] S.K. Sharma, S. Kalia, R.K. Kalia, Rapid in vitro regeneration from 40-year-old clump of Bambusa nutans Wall. ex Munro. Journal of Indian Botanical Society 91 (2012) 365-378
  • [43] R.S. Nadgauda, V.A. Parasharami, A.F. Mascarenhas, Precocious flowering and seeding behaviour in tissue-cultured bamboos. Nature 344 (1990) 335-336
  • [44] S. Saxena, S.S. Bhojwani, In vitro clonal multiplication of 4-yearold plants of the bamboo, Dendrocalamus longispathus Kurz. In Vitro Cellular & Developmental Biology - Plant 29 (1993) 135-142
  • [45] H.C. Chaturvedi, M. Sharma, A.K. Sharma, In vitro regeneration of Dendrocalamus strictus Nees through nodal segments taken from field-grown culms. Plant Sciences 91 (1993) 97-101
  • [46] M. Thiruvengadam, K.T. Rekha, M. Chung, Rapid in vitro micropropagation of Bambusa oldhamii Munro. Philippine Agricultural Scientist 94 (2011) 7-13
  • [47] K. Gillis, J. Gielis, H. Peeters, Somatic embryogenesis from mature Bambusa balcooa Roxburgh as basis for mass production of elite forestry bamboos. Plant Cell Tissue and Organ Culture 91 (2007) 115-123
  • [48] K.T. Cheah, L.C. Chaille, Somatic Embryogenesis From Mature Bambusa ventricosa. Biotechnology BIO-11 (2011) 1-5
  • [49] S. Godbole, A. Sood, R. Thakur, M. Sharma, P. S. Ahuja, Somatic embryogenesis and its conversion into plantlets in a multipurpose bamboo, Dendrocalamus hamiltonii Nees et Arn. Ex Munro. Current Science 83(2002) 885-889
  • [50] X. Lin, L. Huang, W. Fang, Bamboo Regeneration via Embryogenesis and Organogenesis, Embryogenesis, Dr. Ken-Ichi Sato (Ed.), (2012) ISBN: 978-953-51-0466-7
  • [51] Y. Jin-Ling, Jin-Jun Y, Xiao-Li W, Xiao-Ping G, Protocol for Callus Induction and Somatic Embryogenesis in Moso Bamboo. PLoS ONE 8(2013) e81954
  • [52] I.D. Arya, S. Arya, In vitro shoot proliferation and somatic embryogenesis: means of rapid bamboo multiplication. 10th World Bamboo Congress, Propagation, Plantation and Management, Korea 2015,
  • [53] P.V. Somashekar, T.S. Rathore, K.S. Shashidhar, Rapid and simplified method of micropropagation of Pseudoxytenanthera stocksii. In: Ansari SA, Narayanan C, Mandal AK (eds) Forest biotechnology in India. Satish Serial Publishing House, Delhi, (2008) 165–182
  • [54] M.S. Shekhawat, N.S. Shekhawat, Micropropagation of Arnebia hispidissima (Lehm). DC. and production of alkannin from callus and cell suspension culture. Acta Physiologiae Plantarum 33(2011) 1445-1450
  • [55] M. Das, A. Pal, In vitro regeneration of Bambusa balcooa Roxb.: Factors affecting changes of morphogenetic competence in the axillary buds. Journal of Plant Biochemistry and Biotechnology 14 (2005) 185-188
  • [56] R. Yasodha, R. Sumathi, P. Malliga, K. Gurumurthi, Genetic enhancement and mass production of quality propagules of Bambusa nutans and Dendrocalamus membranaceous. Indian Forester 123 (1997) 303-306
  • [57] R. Yasodha, S. Kamala, S.P.A. Kumar, P.D. Kumar, K. Kalaiarasi, Effect of glucose on in vitro rooting of mature plants of Bambusa nutans. Scientia Horticulturae 116 (2008) 113-116
  • [58] P.V. Somashekar, T.S. Rathore, T. Fatima, In vitro plant regeneration of Dendrocalamus stocksii (Munro) M. Kumar, Remesh & Unnikrisnan, through Somatic Embryogenesis. American Journal of Plant Sciences 9 (2018) 2429-2445
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