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2016 | 21 | 104-108
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The aim of the study was determine activity of chitosan ascorbate against 17 strains of Helicobacter pylori. The strains were isolated from gingival pockets (7 strains), atherosclerotic plaques from carotid and femoral (10 strains). Chitosan was obtained from krill chitin and deacetylation degree was equal 60%. The ratio of ascorbic to chitosan was equal 1. The susceptibility of rods was determined by means of plate dilution technique in Brucella agar with 5% sheep’s blood. The inoculum contained 105 CFU/spot. Incubation was performed in anaerobic jars with Campy Pak (BBL) for 48 hrs. The MIC was interpreted as the lowest concentration of chitosan ascorbate inhibiting the growth of bacteria. The results indicated, that chitosan ascorbate at the lowest concentrations was active against 35% of the strains. The Helicobacter pylori rods isolated from gingival pockets were the highest susceptible than isolated from atherosclerotic plaques.
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  • Department of Oral Microbiology, Medical University of Gdańsk, Do Studzienki str. 38, 80-227 Gdańsk, Poland
  • Department of Conservative Dentistry, Medical University of Gdańsk, E. Orzeszkowej str. 18, 80-208 Gdańsk, Poland
  • Department of Oral Microbiology, Medical University of Gdańsk, Do Studzienki str. 38, 80-227 Gdańsk, Poland
  • Department of Oral Microbiology, Medical University of Gdańsk, Do Studzienki str. 38, 80-227 Gdańsk, Poland
  • Department of Vascular Surgery, Swissmed Hospital, Wileńska str. 44, 80-215 Gdańsk, Poland
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