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Dose and Time Dependent Response of HL - 60 Cells Against Newly Isolated Phenolic Compound from Pandanus odoratissimus

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Bioassay guided chromatographic purification of methanolic extract of Pandanus odoratissium leaves afforded new phenolic compound. The isolated compound was identified as 4-(4-(3,4-dimethoxyphenyl) hexahyddrofuro[3,4-c]furan-l-yl)-2-methoxyphenyl acetate by the spectroscope methods. The puropose of this investigation was to check the effect of the phenolic compound 4-(4-(3, 4-dimethoxyphenyl)hexahyddrofuro[3,4-c]furan-l-yl)-2-methoxyphenyl acetate isolated pure compound, the human leukemia (HL-60) cell line as model to evaluate the cytoxicity of compound on the MTT assay. The results obtained from the parameter indicates the compound has significantly reduced the viability of HL-60 cells shows LD50 values of 21.24 ± 0.5 μg/mL, 10.25 ± 0.3 μg/mL and 5.2 ± 0.6 μg/mL, upon 12, 24 and 48 hours of exposure respective indicates a dose and dependent response relationship. The present study indicate the compound is highly cytotoxic to human leukemia (HL-60) cells supports it as an effective therapeutic agent against leukemia.
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  • [1] Zahra Amirghofram, Masoud Bahmani, Abbas Azadmehr, Katayoun Javidnia. Induction of apoptosis in leukemia cell lines by Linum persicum and Euphorbia cheiradenia. Journal of Res Clin Oncol. 132 (2006) 427-432.
  • [2] Grune & Stratton Inc. 1987. Fried, Edrita Publisher: New York.
  • [3] Warrell, R. P. Jr. Pathogenesis and management of acute promoyelocytic leukemia. Annu Rev Med. 47 (1996) 555.
  • [4] M. Omary, I. Trowbridgy, J. Minowada: Human cell surface glycoproteins with unsual propertie. Nature 286 (1980) 888.
  • [5] R. Sutherland, D. Delia, C. Schenider, R. Newman, J. Kemshead, M. Greaves: Ubiquitous cell surface glycoprotein on tumor cells is proliferation associated receptor for transferring. Proc Natl Acad Sci USA 78 (1981) 4515.
  • [6] R. Taetle, J. Honeysett, I. Trowbridge: Effects on antitransferrin receptor antibodies on growth of normal and malignant myeloid cells. Intl J Cancer 32 (1983) 343.
  • [7] T. Chaplinski, T. Bennett, J. Caro: Alteration in insulin receptor expression accompanying differentiation of HL-60 leukemia cells. Cancer Res 46 (1986) 1203.
  • [8] F. Ruscetti, S. Collins, A Woods, R. Gallo: Clonal analyses of the response of human myeloid leukemia cell lines to colony stimulating activity. Blood 58 (1981) 285.
  • [9] D.Metcalf: Clonal analysis of the response of Hl-60 human myeloid leukemia cells to biologic regulators. Leuk Res 7 (1983) 117.
  • [10] M. Tomonga, D. Golde, L. Gasson: Biosynthetic (recombinant) human granulocyte- macrophage colony stimulating factor: Effect on normal bone marrow and leukemia cell lines. Blood 67 (1986) 31.
  • [11] D. Morgan, F. Russetti, R. Gallo: selective in vitro growth of T lymphocytes from normal human bone marrows. Science 193 (1976) 1007.
  • [12] Steven J Collins. The HL-60 Promyelocytic leukemia cell lines: Proliferation differentiation and cellular oncogene expression. Blood 70 (1987) 1233-1244.
  • [13] Kirtikar and Basu. 2000 Indian Medicinal Plant, Sri Satguru Publication. Vol. 10 p. 3565
  • [14] R. I. Freshney: Culture of Animal cells. A manual of basic techniques. Alan Liss Inc (Univeristy library), 1993.
  • [15] J. Paul: Cell and Tissue Culture. Churchill Livingstone (CSL). 1975.
  • [16] Mosmann. T: Rapid colorimeteric assay for cellular growth and survival: applications to proliferation and cytotoxicity assays. J Immuno Methods. 65(1-2) (1983) 55-63.
  • [17] P. B. Tchounwou, B. Wilson, J. Schneider, A. Ishaque. Cytogenic assessment of arsenic trioxide toxicity in Mutatox, Amex II and CAT- Tox assays. Metal Ions boil. Med. 6 (2000) 89-91.
  • [18] S. Collins, R.Gallo, R.Gallagher: Continuous growth and differentiation of human myeloid leukemic cells in suspension culture. Nature 270 (1977) 347.
  • [19] P. B. Tchounwou, C. G. Yedjou, W. C. Dorsey. Arsenic trioxide induced transcriptional activation and expression of stress gene in human liver carcinoma cells (HepG2). Cellular and molecular biology. 49(7) (2003) 1071- 1079.
  • [20] S. L. Soignet, S. R Frankel, D. Douer, M. S. Tallman, H. Kantarjian, E. Calleja, R. M. Stone, M. Kalaycio, D. A. Scheinberg, P. Steinherz, E. L. Sievers, S. Coutre, S. Dahlberg, R. Ellison, R. P. Warell. United States multicenter study of arsenic trioxide in relapsed acute promyelocytic leukemia. J. Clin. Oncol. 19 (2001) 3852-3860.
  • [21] P. Fenaux, C. Chomienne, L. Degos: Treatment of acute promyelocytic leukaemia. Clin Haematol. 14 (2001) 153-174.
  • [22] J. Mayorga, C. Richarson-Hardin, K.A. Dicke: Arsenic trioxide as effective therapy for relapsed acute promyelocytic leukemia. Clin J Oncol Nurs. 6(6) (2002) 341-346.
  • [23] Ting ting Jong and Shang Whang Chau. (1998). Antioxidative activites of constituents isolated from Pandanus odoratissimus. Phytochemistry. 49(7): 2145-2148.
  • [24] Yamaguchi, K. (1970). Spectral data of Natural Products Vol. I Elsevier Publishing Company, Amsterdam, London, New York.
  • [25] NAPRALERT, 1993. Biological activities for extracts of Pandanus and ethnomedical information on Pandanus. University of Illinois at Chicago. Chicago, IL.
  • [26] Londonkar R and A Kamble. 2009. Evaluation of Free Radical Scaveniging activity of Pandanus odoratissimus. International Journal of Pharmacology 5 (6) 377-380.
  • [27] Wilson. K and Walker. J. (1995). Practical Biochemistry; Principles and techniques, 4th Edn. Published by Cambridge University Press, Cambridge, UK. Pp 586.
  • [28] Ting ting Jong and Shang Whang Chau. (1998). Antioxidative activites of constituents isolated from Pandanus odoratissimus. Phytochemistry. 49(7): 2145-2148.
  • [29] Yamaguchi, K. (1970). Spectral data of Natural Products Vol. I Elsevier Publishing Company, Amsterdam, London, New York..
  • [30] Keerthikar and Basu. (2000). Indian Medicinal Plant, Sri Satguru Publication. Vol. 10 p. 3565.
  • [31] Warrier R. K., V. P. K. Nambiar and C. Ramankutty, 1995. Indian Medicinal Plants: a compendium of 500 species: Orient Longman Ltd. Madras. India.
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