PL EN


Preferences help
enabled [disable] Abstract
Number of results
2015 | 10 | 94-110
Article title

Dose and Time Dependent Response of HL - 60 Cells Against Newly Isolated Phenolic Compound from Pandanus odoratissimus

Content
Title variants
Languages of publication
EN
Abstracts
EN
Bioassay guided chromatographic purification of methanolic extract of Pandanus odoratissium leaves afforded new phenolic compound. The isolated compound was identified as 4-(4-(3,4-dimethoxyphenyl) hexahyddrofuro[3,4-c]furan-l-yl)-2-methoxyphenyl acetate by the spectroscope methods. The puropose of this investigation was to check the effect of the phenolic compound 4-(4-(3, 4-dimethoxyphenyl)hexahyddrofuro[3,4-c]furan-l-yl)-2-methoxyphenyl acetate isolated pure compound, the human leukemia (HL-60) cell line as model to evaluate the cytoxicity of compound on the MTT assay. The results obtained from the parameter indicates the compound has significantly reduced the viability of HL-60 cells shows LD50 values of 21.24 ± 0.5 μg/mL, 10.25 ± 0.3 μg/mL and 5.2 ± 0.6 μg/mL, upon 12, 24 and 48 hours of exposure respective indicates a dose and dependent response relationship. The present study indicate the compound is highly cytotoxic to human leukemia (HL-60) cells supports it as an effective therapeutic agent against leukemia.
Publisher

Year
Volume
10
Pages
94-110
Physical description
Contributors
  • Department of Biotechnology, Gulbarga University, Gulbarga, Karnataka - 585106, India, abhay.gene@gmail.com
  • Department of Biotechnology, Gulbarga University, Gulbarga, Karnataka - 585106, India
  • Department of Biotechnology, Gulbarga University, Gulbarga, Karnataka - 585106, India
References
  • [1] Zahra Amirghofram, Masoud Bahmani, Abbas Azadmehr, Katayoun Javidnia. Induction of apoptosis in leukemia cell lines by Linum persicum and Euphorbia cheiradenia. Journal of Res Clin Oncol. 132 (2006) 427-432.
  • [2] Grune & Stratton Inc. 1987. Fried, Edrita Publisher: New York.
  • [3] Warrell, R. P. Jr. Pathogenesis and management of acute promoyelocytic leukemia. Annu Rev Med. 47 (1996) 555.
  • [4] M. Omary, I. Trowbridgy, J. Minowada: Human cell surface glycoproteins with unsual propertie. Nature 286 (1980) 888.
  • [5] R. Sutherland, D. Delia, C. Schenider, R. Newman, J. Kemshead, M. Greaves: Ubiquitous cell surface glycoprotein on tumor cells is proliferation associated receptor for transferring. Proc Natl Acad Sci USA 78 (1981) 4515.
  • [6] R. Taetle, J. Honeysett, I. Trowbridge: Effects on antitransferrin receptor antibodies on growth of normal and malignant myeloid cells. Intl J Cancer 32 (1983) 343.
  • [7] T. Chaplinski, T. Bennett, J. Caro: Alteration in insulin receptor expression accompanying differentiation of HL-60 leukemia cells. Cancer Res 46 (1986) 1203.
  • [8] F. Ruscetti, S. Collins, A Woods, R. Gallo: Clonal analyses of the response of human myeloid leukemia cell lines to colony stimulating activity. Blood 58 (1981) 285.
  • [9] D.Metcalf: Clonal analysis of the response of Hl-60 human myeloid leukemia cells to biologic regulators. Leuk Res 7 (1983) 117.
  • [10] M. Tomonga, D. Golde, L. Gasson: Biosynthetic (recombinant) human granulocyte- macrophage colony stimulating factor: Effect on normal bone marrow and leukemia cell lines. Blood 67 (1986) 31.
  • [11] D. Morgan, F. Russetti, R. Gallo: selective in vitro growth of T lymphocytes from normal human bone marrows. Science 193 (1976) 1007.
  • [12] Steven J Collins. The HL-60 Promyelocytic leukemia cell lines: Proliferation differentiation and cellular oncogene expression. Blood 70 (1987) 1233-1244.
  • [13] Kirtikar and Basu. 2000 Indian Medicinal Plant, Sri Satguru Publication. Vol. 10 p. 3565
  • [14] R. I. Freshney: Culture of Animal cells. A manual of basic techniques. Alan Liss Inc (Univeristy library), 1993.
  • [15] J. Paul: Cell and Tissue Culture. Churchill Livingstone (CSL). 1975.
  • [16] Mosmann. T: Rapid colorimeteric assay for cellular growth and survival: applications to proliferation and cytotoxicity assays. J Immuno Methods. 65(1-2) (1983) 55-63.
  • [17] P. B. Tchounwou, B. Wilson, J. Schneider, A. Ishaque. Cytogenic assessment of arsenic trioxide toxicity in Mutatox, Amex II and CAT- Tox assays. Metal Ions boil. Med. 6 (2000) 89-91.
  • [18] S. Collins, R.Gallo, R.Gallagher: Continuous growth and differentiation of human myeloid leukemic cells in suspension culture. Nature 270 (1977) 347.
  • [19] P. B. Tchounwou, C. G. Yedjou, W. C. Dorsey. Arsenic trioxide induced transcriptional activation and expression of stress gene in human liver carcinoma cells (HepG2). Cellular and molecular biology. 49(7) (2003) 1071- 1079.
  • [20] S. L. Soignet, S. R Frankel, D. Douer, M. S. Tallman, H. Kantarjian, E. Calleja, R. M. Stone, M. Kalaycio, D. A. Scheinberg, P. Steinherz, E. L. Sievers, S. Coutre, S. Dahlberg, R. Ellison, R. P. Warell. United States multicenter study of arsenic trioxide in relapsed acute promyelocytic leukemia. J. Clin. Oncol. 19 (2001) 3852-3860.
  • [21] P. Fenaux, C. Chomienne, L. Degos: Treatment of acute promyelocytic leukaemia. Clin Haematol. 14 (2001) 153-174.
  • [22] J. Mayorga, C. Richarson-Hardin, K.A. Dicke: Arsenic trioxide as effective therapy for relapsed acute promyelocytic leukemia. Clin J Oncol Nurs. 6(6) (2002) 341-346.
  • [23] Ting ting Jong and Shang Whang Chau. (1998). Antioxidative activites of constituents isolated from Pandanus odoratissimus. Phytochemistry. 49(7): 2145-2148.
  • [24] Yamaguchi, K. (1970). Spectral data of Natural Products Vol. I Elsevier Publishing Company, Amsterdam, London, New York.
  • [25] NAPRALERT, 1993. Biological activities for extracts of Pandanus and ethnomedical information on Pandanus. University of Illinois at Chicago. Chicago, IL.
  • [26] Londonkar R and A Kamble. 2009. Evaluation of Free Radical Scaveniging activity of Pandanus odoratissimus. International Journal of Pharmacology 5 (6) 377-380.
  • [27] Wilson. K and Walker. J. (1995). Practical Biochemistry; Principles and techniques, 4th Edn. Published by Cambridge University Press, Cambridge, UK. Pp 586.
  • [28] Ting ting Jong and Shang Whang Chau. (1998). Antioxidative activites of constituents isolated from Pandanus odoratissimus. Phytochemistry. 49(7): 2145-2148.
  • [29] Yamaguchi, K. (1970). Spectral data of Natural Products Vol. I Elsevier Publishing Company, Amsterdam, London, New York..
  • [30] Keerthikar and Basu. (2000). Indian Medicinal Plant, Sri Satguru Publication. Vol. 10 p. 3565.
  • [31] Warrier R. K., V. P. K. Nambiar and C. Ramankutty, 1995. Indian Medicinal Plants: a compendium of 500 species: Orient Longman Ltd. Madras. India.
Document Type
article
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.psjd-c9c1176b-6477-4d9c-8b9a-49a61b08a395
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.