THE INHIBITION OF HUMAN PLATELET AGGREGATION BY A LOW-MOLECULAR WEIGHT CHITOSAN

• Authors: Natalia N Drozd , Yulia S. Logvinova , Balzhima Ts. Shagdarova , Alla V. Il’ina , Valery P. Varlamov
• Languages of publication: EN

Abstracts

EN

Chitosan derivatives were obtained by chemical (MW of 6 kDa, DD 99% - Ch6/99; MW13 kDa, DD 98% - Ch13/98) and enzymatic (MW of 5 kDa, DD 85% - Ch5/85; MW of 10 kDa, DD 85% - Ch10/85) depolymeri-sation of chitosan with a MW of 334 and 1000 kDa. Chitosan derivatives (almost identical MW pairs and different DD) possessed insignificant an-ticoagulant activity, did not promote human platelet aggregation and re-duced ADP or collagen-induced platelet aggregation. The studied sam-ples at a concentration of 2 mg/ml reduced the aggregation of platelets more than twice induced in 2x10-6M and 1x10-5M concentrations; at weak activation in 2x10-6M, the Ch10/85 sample was the most effective. The Ch6/99 and Ch13/98 samples were 20 times more effective at the inhibi-tion of collagen-induced platelet aggregation than the Ch10/85 sample. The latter can be explained by the greater value of positive charge (DD) and polydispersity (Mw/Mn) of chitosan samples obtained by chemical de-polymerisation

Keywords

EN

chitosan; deacetylation degree; depolymerisation; molecular weight; platelet aggregation

Discipline

• IMMUNOLOGY: IMMUNOLOGY

• Publisher: Sieć Badawcza Łukasiewicz - Polskie Towarzystwo Chitynowe
• Journal: Progress on Chemistry and Application of Chitin and its Derivatives
• Year: 2018
• Volume: 23
• Pages: 55 - 65

Contributors

author

Natalia N Drozd

• National Research Centre for Haematology of the Ministry of Healthcare of the Russian Federation

author

Yulia S. Logvinova

• National Research Centre for Haematology of the Ministry of Healthcare of the Russian Federation

author

Balzhima Ts. Shagdarova

• Institute of Bioengineering, Research Centre of Biotechnology of the Russian Academy of Sciences

author

Alla V. Il’ina

• Institute of Bioengineering, Research Centre of Biotechnology of the Russian Academy of Sciences

author

Valery P. Varlamov

• Institute of Bioengineering, Research Centre of Biotechnology of the Russian Academy of Sciences

References

• [1] Anitha A, Sowmy S, Kumar PTS, Deepthi S, Chennazhi KP, Ehrlich H, Tsurkan M, Jayakumar R; (2014) Chitin and chitosan in selected biomedical applications. Prog Polym Sci. DOI:.org/10.1016/j.progpolymsci.2014.02.008
• [2] Szymańska E, Winnicka K; (2015) Stability of Chitosan—A Challenge for Phar-maceutical and Biomedical Applications. Mar. Drugs DOI:10.3390/md13041819
• [3] de Moura FA, Macagnan FT, da Silva LP; (2015) Oligosaccharide production by hydrolysis of polysaccharides: a Review. IJFST. DOI:10.1111/ijfs.12681
• [4] Kasaai MR, Arul J, Charlet G; (2013) Fragmentation of Chitosan by Acids. The ScientificWorld Journal. .http://dx.doi.org/10.1155/2013/508540
• [5] Mourya VK,. Inamdar NN, Y.M.Choudhari YM; (2011) Chitooligosaccha-rides:Synthesis, Characterization and Applications. Polym. Sci. Ser. A. DOI: 10.1134/S0965545X11070066
• [6] Kim HB, Lee J, Oh SH, Kang PH, Jeun JP; (2013) Molecular Weight Control of Chitosan Using Gamma Ray and Electron Beam Irradiation. Journal of Radiation Industry. 2013. 7, 51-54
• [7] Vasilieva T, Chuhchin D, Lopatin S, Varlamov V, Sigarev A, Vasiliev M; (2017) Chitin and Cellulose Processing in low-temperature electron beam plasma. Mole-cules. DOI:10.3390/molecules22111908
• [8] Roncal T, Oviedo A, de Armentia IL, Fernandez L, Villaran MC; (2007) Carbo-hydr. Res. Doi:10.1016/j.carres.2007.08.023
• [9] Tsao CT, Chang CH, LinYY, Wuc MF, Han JL, His KH; (2011) Kinetic study of acid depolymerization of chitosan and effects of low molecular weight chitosan on erythrocyte rouleaux formation. Carbohydr. Res. DOI:10.1016/j.carres.2010.10.010
• [10] Xia W,.Liu P, Zhang J, Chen J; (2011) Biological activities of chitosan and chitooligosaccharide. Food Hydrocoll. DOI:10.1016/j.foodhyd.2010.03.003
• [11] Skorik Y, Kritchenkov A, MoskalenkoY, Golyshev A, Raik S, Whaley A, Vasina L, Sonin D; (2017) Synthesis of N-succinyl- and N-glutaryl-chitosan derivatives and their antioxidant, antiplatelet, and anticoagulant activity. Carbohydr Polym. DOI: 10.1016/j.carbpol.2017.02.097
• [12] Wang T, Zhou Y, Xie W, Chen L, Zheng H, Fan L; (2012) Preparation and anticoagulant activity of N-succinyl chitosan sulfates. Int J Biol Macromol. DOI: 10.1016/j.ijbiomac.2012.07.029
• [13] Sharma A, Hai O, Garg A, Vallakati A, Lavie C, Marmur J; (2017) Duration of Dual Antiplatelet Therapy Following Drug-Eluting Stent Implantation: A Systmatic Review and Meta-analysis of Randomized Controlled Trials. Curr Probl Cardiol. DOI: 10.1016/j.cpcardiol.2017.04.001
• [14] Olaf M, Cooney R; (2017) Deep Venous Thrombosis. Emerg Med Clin North Am. DOI: 10.1016/j.emc.2017.06.003
• [15] Zhang Q, Lu X, Yang S, Zhang Q, Zhao L; (2017) Preparation of anticoagulant polyvinylidene fluoride hollow fiber hemodialysis membranes. Biomed Eng Biomed Tech. DOI: 10.1515/bmt-2015-0149
• [16] Kim ES, Lee JS, Lee HG; (2016) Nanoencapsulation of Red Ginseng Extracts Us-ing Chitosan with Polyglutamic Acid or Fucoidan for Improv-ing Antithrombotic Activities. J Agric Food Chem. DOI: 10.1021/acs.jafc.6b00911
• [17] Khasanova LM, Il’ina AV, Varlamov VP, Sinitsyna OA, Sinitsyn AP; (2014) Hy-drolysis of Chitozan with an Enzyme Complex from Myceliophthora sp. Applied Biochemistry and Microbiology. DOI: 10.1134/S0003683814040061
• [18] Kulikov S, Tikhonov V, Blagodatskikh I, Bezrodnykh E, Lopatin S, Khairullin R, Philippova Y, Abramchuk S; (2012) Molecular weight and pH aspects of the ef-ficacy of oligochitosan against meticillin-resistant Staphylococcus aureus (MRSA). Carbohydr Polym. DOI:10.1016/j.carbpol.2011.08.017
• [19] Reno W, Rotman M, Grumbini F, Dennis L., Mohler E; (1974) Evaluation of the BART test (a modification of the whole blood activated recalcification time test) as a means of monitoring heprin therapy. Am J Clin Pathol 61, 78-84. PMID: 4809149
• [20] Teien A, Lie M; (1975) Heparin assay in plasma a comparison of five clotting methods. Thromb Res 7, 777 – 788. PMID: 1209561
• [21] Ingram G, Hills M; (1976) Reference method for the one-stage prothrombin time test on human blood . Thrombosis Haemostasis 36, 237-238. PMID: 1036814
• [22] Born G; (1962) Aggregation of blood platelets by adenosine diphosphate and its reversal. Nature 194, 927-929. PMID: 13871375
• [23] Roncal T, Oviedo A, de Armentia IL, Fernandez L, Villarn MC; (2007) High yield production of monomer-free chitosan oligosaccharides by pepsin catalyzed hydrol-ysis of a high deacetylation degree chitosan. Carbohydr Res. DOI:10.1016/j.carres.2007.08.023
• [24] Lord M, Cheng B, McCarthy SJ, Jung M, Whitelock JM; (2011) The modulation of platelet adhesion and ativation by chitosan through plasma and extracellular matrix proteins. Biomaterials. DOI: 10.1016/j.biomaterials.2011.05.062
• [25] Chou TC, Fu E, Wu CJ, Yeh JH; (2003) Chitosan enhances platelet adhesion and aggregation. Biochem Biophys Res Com. DOI:org/10.1016/S0006-291X(03)00173-6
• [26] Ollivier V, Syvannarath V, Gros A, Butt A, Loyau S, Jandrot-Perrus M, Ho-Tin-Noé B; (2014) Collagen can selectively trigger a platelet secretory phenotype via glycoprotein VI. PLoS One. DOI:10.1371/journal.pone.0104712
• [27] Polyakova AM, Astrina OS, Bakhtina YuA, Maleev VV, Ermak IM, Barabanova AO (2005) Possibility of correction of functional state of human thrombocytes with natural polysaccharides under the conditions of experimental endotoxemia and in patients with food poisoning. Infectious diseases 3, 44-46. https://elibrary.ru/item.asp?id=9510960
• [28] Jian Y, Feng T, Zheng W, Qing W, Yan-Jun Z, Shi-Qian C; (2008) Effect of Chi-tosan Molecular Weight and Deacetylation Degree on Hemostasis. J Biomed Mater Res B DOI:10.1002/jbm.b.30853

• Document Type: article