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This work presents, for the first time, the extraction and characterization of chitin from the shell of the freshwater crab species Potamon algeriense with a standardized and revised chemical method. Chitin and chitosan were isolated following demineralization, deproteinization, decolouration (raw chitin), and deacetylation (chitosan). After boiling, drying, and grinding, 62.12% of the ground shell was obtained. A yield of 40.92% was obtained after demineralization of ground crab shell, while after the deproteinization process 8.74% was obtained. After decolourization, 8.27% of raw chitin was obtained, and the final amount of chitosan extracted from the crab shells was approximately 5.89%. We also characterized the isolated chitin by determining its physicochemical properties using X-ray powder diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA).
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  • 1Laboratory of Water, Environment, and Sustainable Development, Mohamed First University
  • Biochemistry and Biotechnology Laboratory, Mohamed First University,
  • Laboratory of mineral solid and analytical chemistry, Mohamed First University,
  • Laboratory of Physics of Matter and Radiation, Mohamed First University,
  • Laboratory of Water, Environment, and Sustainable Development, Mohamed First University,
  • Laboratory of Water, Environment, and Sustainable Development, Mohamed First University,
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