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2018 | 108 | 123-132
Article title

Immunostimulation effect of extract ethanol white oyster mushroom (Pleurotus ostreatus Jacq Fr. Kumm) on mice

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Acknowledging the immunostimulation effect of ethanol extract white oyster mushroom to animal experiments which were desensitized with red blood cell of lamb. This study is an experimental study, 14 healthy male swiss Webster mices were used. The subjects are randomly divided into two groups. The first group is a control group and the second group is a treatment group. The immunostimulation effect is assessed by specific immune response to desensitized of lamb’s RBC. Humoral immune response was assessed through a hemagglutination test. Cellular immune response was assessed with a delayed type hypersensitivity test by measuring the thickness of edema of the mice’s foot Specific immune response is assessed through total of blood lymphocyte using flowcytometry method. We found an increase of total blood lymphocyte level on the primary sensitization of placebo and Ethanol extract of white oyster mushroom (EEWOM). After secondary sensitization, the total blood lymphocyte level on placebo and EEWOM showed a significant difference. EEWOM increases the total antibody titre significantly compare to placebo. The level of serum gamma-globulin of treatment mice is significantly higher than placebo. On the 24th and 48th hour after the sensitization of lamb’s RBC, the increase of thickness of the mice's feet which were given placebo and EEWOM have significant difference. EEWOM has an immunostimulating effect towards specific immune response.
Physical description
  • Biomedical Scinces Master Program, Faculty of Medicine, Universitas Padjadjaran, Bandung, West Java, Indonesia
  • Biomedical Scinces Master Program, Faculty of Medicine, Universitas Padjadjaran, Bandung, West Java, Indonesia
  • Faculty of Medicine, Universitas Padjadjaran, Bandung, West Java, Indonesia
  • Department of Basic Medical Science, Faculty of Medicine, Universitas Padjadjaran, Bandung, West Java, Indonesia
  • Department of Basic Medical Science, Faculty of Medicine, Universitas Padjadjaran, Bandung, West Java, Indonesia
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