Preferences help
enabled [disable] Abstract
Number of results
2017 | 67 | 2 | 265-276
Article title

Isolation of DNA from bone material of selected animals from Cervidae family

Title variants
Languages of publication
Bone material is one of the most difficult biological samples used for extraction of DNA. The primary objective of molecular tests is to determine the concentration and quality of DNA, and these results are of significant importance in further analyses. The aim of the study was to evaluate the usefulness of a method for DNA isolation from bone material of selected animal species from Cervidae family using a commercially available GeneMatrix Bone DNA Purification Kit manufactured by Eurx. Quantitative evaluation was performed with a spectrophotometer. Pure DNA isolates were obtained from 21 out of 24 bone fragments. The efficiency of the applied isolation method varied. The difference between the lowest and highest DNA concentration was more than hundredfold. Qualitative analysis was carried out by means of electrophoresis in 1% agarose gel. No high molecular mass DNA was obtained. The genetic material was present in small quantity and it was fragmented. It was concluded that the Bone DNA Purification Kit manufactured by Eurx may be effective only in isolation of DNA from bones stored in good conditions.
Physical description
  • [1] R. Alaeddini., S. J. Walsh, A. Abbas. Forensic implications of genetic analyses from degraded DNA - a review. Forensic Science International. Genetics 4(3) (2010) 148-57.
  • [2] Alonso, S. Andelinovic, P. Martín, D. Sutlovic, I. Erceg, E. Huffine, L. Fernández de Simón, C. Albarrán, M. Definis-Gojanovic, A. Fernández-Rodriguez, P. García, I. Drmic, B. Rezic, S. Kuret, M. Sancho, D. Primorac, DNA typing from skeletal remains: evaluation of multiplex and megaplex STR systems on DNA isolated from bone and teeth samples. Croatian Medical Journal, 42(3) (2001) 260-6.
  • [3] J. Davoren, D. Vanek, R. Konjhodzić J. Crews, E. Huffine, T. J. Parsons. Highly effective DNA extraction method for nuclear short tandem repeat testing of skeletal remains from mass graves. Croatian Medical Journal, 48(4) (2007) 478-85.
  • [4] M. Dębska. Bieżące kierunki w kryminalistycznych badaniach dotyczących identyfikacji zwierząt należących do gatunków zagrożonych wyginięciem. Problemy Kryminalistyki 280(2) (2013) 28-38.
  • [5] P. Gawin, D. Durbas-Nowak, J. Pełka, D. Bogacz, D. Dudkiewicz. Łowiectwo: tradycje, prawo, zwierzyna. Warszawa, SBM. 2015. 64-79.
  • [6] O. M. Loreille, T. M. Diegoli, J. A. Irwin, M. D. Coble, T. J. Parsons. High efficiency DNA extraction from bone by total demineralization. Forensic Science International: Genetics, 1(2) (2007) 191-195.
  • [7] R. Słomski. Analiza DNA, teoria I praktyka. Poznań. UP. 2014.
  • [8] R. Słomski, A. Dzieduszycki, D. Lipiński, M. Szalata, J. Zeyland, K. Wielgus, H. Frąckowiak, Z. Smorąg, M. Ryba. Analiza DNA tura (Bos primigenius) Nauka 4/2008, 65-75.
  • [9]
Document Type
Publication order reference
YADDA identifier
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.