Comparative Anti-Bacterial Effect of Aqueous and Ethanolic Stem Barks of Tamarindus indica Linn on Staphylococcus aureus

• Authors: David Itopa Boyi , Lawan Abdu Sani
• Languages of publication: EN

Abstracts

EN

This study focuses on Tamarindus indica, a plant belonging to family Fabaceae commonly found in Nigeria and the Asian continent, and explores the potential comparative antibacterial activity of its aqueous and ethanolic stem barks against Staphylococcus aureus, a Gram-positive bacterium. The stem barks of Tamarindus indica were obtained from a live Tamarind tree located at Bayero university Kano, old campus, was dried under shade away from direct sunlight in the laboratory, pounded to fine powder using mortar and pestle and extracted through maceration method using distilled water and ethanol solvents. Phytochemical analysis was carried out following standard procedures and it revealed the presence of alkaloids, flavonoids, tannins, saponins, phenols, terpenoids, steroids, glycosides, and carbohydrates in the extracts. Agar well diffusion method was employed to assess the antibacterial activity. The results showed the aqueous extracts exhibited bigger zones of inhibition compared to the ethanol extracts. Furthermore, the lowest MIC was 15.625 mg/ml for ethanolic Stem bark extract revealing how effective the stem bark ethanol extracts were in combating the bacteria. The study demonstrated that Tamarindus indica stem barks possesses antibacterial properties which may be attributed to the presence of bioactive compounds such as alkaloids, flavonoids, tannins, and phenols and highlights the potential of Tamarindus indica as an affordable and accessible natural remedy against Staphylococcus aureus infections and diseases.

Keywords

EN

Antibacterial; Staphylococcus aureus; Tamarindus indica; bactericidal; inhibitory

Discipline

• BIOLOGY: BIOLOGY

• Publisher: Scientific Publishing House „DARWIN”
• Journal: World News of Natural Sciences
• Year: 2024
• Volume: 55
• Pages: 1-15

Contributors

author

David Itopa Boyi

• Department of Plant Biology, Bayero University Kano, Kano State, Nigeria

author

Lawan Abdu Sani

• Department of Plant Biology, Bayero University Kano, Kano State, Nigeria

References

• [1] Abubakar MG, Ukwuani AN, Shehu RA (2008). Phytochemical screening and antibacterial activity of Tamarindus indica pulp extract. Asian Journal of Biochemistry, 3, 134-138
• [2] Adeniyi, O. V., Olaifa, F. E., Emikpe, B. O., & Ogunbanwo, S. T. (2017). Phytochemical components and antibacterial activity of Tamarindus indica Linn. extracts against some pathogens. Environment, 7(14).
• [3] Agyare, C., Dwobeng, A. S., Agyepong, N., Boakye, Y. D., Mensah, K. B., Ayande, P. G., ... & Adarkwa-Yiadom, M. (2013). Antimicrobial, antioxidant, and wound healing properties of Kigelia Africana (Lam.) Beneth. and Strophanthus hispidus DC. Advances in Pharmacological Sciences, 2013: 692613. doi: 10.1155/2013/692613
• [4] Akihisa, T., Koike, K., Kimura, Y., Sashida, N., Matsumoto, T., Ukiya, M., & Nikaido, T. (1999). Acyclic and incompletely cyclized triterpene alcohols in the seed oils of Theaceae and Gramineae. Lipids, 34(11), 1151-1175
• [5] Algammal, A. M., Hetta, H. F., Elkelish, A., Alkhalifah, D. H. H., Hozzein, W. N., Batiha, G. E. S., ... & Mabrok, M. A. (2020). Methicillin-Resistant Staphylococcus aureus (MRSA): one health perspective approach to the bacterium epidemiology, virulence factors, antibiotic-resistance, and zoonotic impact. Infection and Drug Resistance, 13, 3255-3265
• [6] Alrasheid, A. A., Ahmed, S. A., Eltilib, S. H., Alnour, M. I., Widdatallh, M. O., Yassin, L. F., ... & Ayoub, S. M. H. (2019). The effect of blending of extracts of Sudanese Adansonia digitata and Tamarindus indica on their antioxidant, anti-inflammatory and antimicrobial activities. Journal of Pharmacognosy and Phytotherapy, 11(2), 28-34
• [7] Amann, R., & Peskar, B. A. (2002). Anti-inflammatory effects of aspirin and sodium salicylate. European Journal of Pharmacology, 447(1), 1-9
• [8] Anbalahan, N. (2017). Pharmacological activity of mucilage isolated from medicinal plants. International Journal of Applied and Pure Science and Agriculture, 3(1), 98-113
• [9] Asif, A., Asghar, M., Khan, H. U., Haq, I., Shuaib, S. L., Khalid, F., ... & Rehman, N. (2021). Antibiotic susceptibility pattern of clinical isolates of methicillin resistant Staphylococcus aureus in Peshawar, Pakistan. Annals of the Romanian Society for Cell Biology, 25(6), 20116-20131
• [10] Asif, H. M., Akram, M., Saeed, T. M., Khan, I., Akhtar, N., Ur Rehman, R., Ali Shah, S. M., Ahmed, K., & Shaheen, G. (2011). Review paper carbohydrates. International Research Journal of Biochemistry and Bioinformatics, 1(1), 001-005
• [11] Assa, Y., Shany, S., Gestetner, B., Tencer, Y., Birk, Y., & Bondi, A. (1973). Interaction of alfalfa saponins with components of the erythrocyte membrane in hemolysis. Biochimica et Biophysica Acta, 307(1), 83-91
• [12] Avorn, J., Monane, M., Gurwitz, J. H., Glynn, R. J., Choodnovskiy, I., & Lipsitz, L. A. (1994). Reduction of bacteriuria and pyuria after ingestion of cranberry juice. Journal of the American Medical Association, 271(10), 751-754
• [13] Bagci, E., Yazgın, A., Hayta, S., & Cakılcıoglu, U. (2010). Composition of the essential oil of Teucrium chamaedrys L. (Lamiaceae) from Turkey. Journal of Medicinal Plants Research, 4(23), 2587-2589
• [14] Baker, F. A., Silverton, R. E., & Pallinster, C. J. (1993). Introduction to Medical Laboratory Technology: 7th edition, Pp 284-297.
• [15] Bennets, H. W., Underwood, E. J., & Shier, F. L. (1946). A specific breeding problem of sheep in subterranean clover pastures in Western Australia. Australian Veterinary Journal, 22(1), 2-12
• [16] Benowitz, N. L. (1990). Clinical pharmacology of caffeine. Annual Review of Medicine, 41(1), 277-288
• [17] Benowitz, N. L. (2009). Pharmacology of nicotine: Addiction, smoking-induced disease, and therapeutics. Annual Review of Pharmacology and Toxicology, 49, 57-71. DOI: 10.1146/annurev.pharmtox.48.113006.094742
• [18] Bourgaud, F., Gravot, A., Milesi, S., & Gontier, E. (2001). Production of plant secondary metabolites: A historical perspective. Plant Science, 161, 839-851
• [19] Catarino, M. D., Silva, A. M. S., Cruz, M. T., & Cardoso, S. M. (2017). Antioxidant and anti-inflammatory activities of Geranium robertianum L. decoctions. Food and Function, 8(9), 3355-3365. DOI: 10.1039/c7fo00881c
• [20] Cheesbrough, M. (2002). District Laboratory Practice in the Tropical Countries Part 2. Cambridge Low Price Edition, 38-39, 62-70, 143-157
• [21] Cowan, M. M. (1999). Plants products as antimicrobial agents. Clinical Microbiology Reviews, 12(4), 564–582
• [22] de Almeida, J. M., Crippa, B. L., de Souza, V. V. M. A., Alonso, V. P. P., Júnior, E. D. M. S., Picone, C. S. F., ... & Silva, N. C. C. (2023). Antimicrobial action of Oregano, Thyme, Clove, Cinnamon and Black pepper essential oils free and encapsulated against foodborne pathogens. Food Control, 144, 109356
• [23] De, M., De, A. K., & Banerjee, A. B. (1999). Antimicrobial screening of some Indian spices. Phytotherapy Research, 13, 616-618
• [24] Diallo, B. O., Joly, H. I., McKEY, D., Hosaert-McKey, M., & Chevallier, M. H. (2007). Genetic diversity of Tamarindus indica populations: Any clues on the origin from its current distribution?. African Journal of Biotechnology 6(7), 853-860
• [25] Doughari, J. H. (2006). Antibacterial activity of Tamarindus indica linn. Tropical Journal of Pharmaceutical Research, 5(2), 597-603.
• [26] El-Siddig, K. (2006). Tamarind: Tamarindus indica L. ISBN 9780854328598.
• [27] El-siddiq, G., Prassad, P., & Ramana, V. (2006). Tamarindus indica. Southampton UK Center for Underutilized Crops.
• [28] Evans, W. C. (2009). Pharmacognosy (16th ed.). Edinburgh London New York Philadelphia St Louis Sydney Toronto.
• [29] Fahy, E., Subramaniam, S., Murphy, R. C., Nishijima, M., Raetz, C. R., Shimizu, T., ... & Dennis, E. A. (2009). Update of the LIPID MAPS comprehensive classification system for lipids. Journal of Lipid Research, 50(Suppl), S9-S14. DOI: 10.1194/jlr.R800095-JLR200
• [30] Gehm, B. D., McAndrews, J. M., Chien, P., & Jameson, J. L. (1997). Resveratrol, a polyphenolic compound found in grapes and wine, is an agonist for the estrogen receptor. Proceedings of the National Academy of Sciences of the United States of America, 94(25), 14138-14143
• [31] Ghurghure, S. M., Dhange, A. A., Kamalapure, N. R., Kate, S. N., Katkar, A. R., Katta, A. V., ... & Kattimani, S. G. (2019). Formulation and Evaluation of Herbal Hand Wash Gel using Nerium oleander. Research Journal of Topical and Cosmetic Sciences, 10(1), 1-6
• [32] Gonzalez-Lamothe R, Mitchell G, Gattuso M (2009). Plant antimicrobial agents and their effects on plant and human pathogens. Journal of Molecular Sciences, 10, 3400-3419.
• [33] Güçlü-Üstündağ Ö, Mazza G (2007). Saponins: Properties, applications and processing. Critical Reviews in Food Science and Nutrition, 47, 231-258. ISSN: 1040-8398. DOI: 10.1080/ 10408390600698197
• [34] Hagerman AE, Butler LG (1981). The specificity of proanthocyanidin-protein interactions. The Journal of Biological Chemistry, 256(10), 4494-4497
• [35] Hikino H (1985). Recent research on oriental medicinal plants. In: Wanger H, Hiniko H, Farnsworth NR (Eds.), Economic and Medicinal Plant Research, Vol. 1. London: Academic Press. pp. 53-85.
• [36] Hoffmann D (2003). Medical Herbalism: The Science and Practice of Herbal Medicine. Healing Arts Press, One Park Street, Rochester, Vermont. ISBN: 978-089281749-8.
• [37] Hussein RA, El-Anssary AA (2008). Plants Secondary Metabolites: The Key Drivers of the Pharmacological Actions of Medicinal Plants. In: (Eds.) Herbal Medicine. IntechOpen. https://doi.org/10.5772/intechopen.76139.
• [38] Ishikura H, Mochizuki T, Izumi Y, Usui T, Sawada H, Uchino H (1984). Differentiation of mouse leukemic M1 cells induced by polyprenoids. Leukemia Research, 8(5), 843-852
• [39] Jayaweera DM (1981). Medicinal Plants Used in Ceylon. Part III. The National Science Council of Sri Lanka, Colombo.
• [40] Jepson RG, Craig JC (2008). Cranberries for preventing urinary tract infections. Cochrane Database of Systematic Reviews, 1, CD001321. DOI: 10.1002/14651858.CD001321.pub4
• [41] Jones ME, Kossel A (1953). A biographical sketch. Yale Journal of Biology and Medicine, 26(1), 80-97
• [42] Kairey L, Agnew T, Bowles EJ, Barkla BJ, Wardle J, Lauche R (2023). Efficacy and safety of Melaleuca alternifolia (tea tree) oil for human health—A systematic review of randomized controlled trials. Frontiers in Pharmacology, 14, 31
• [43] Korpinen RI, Välimaa AL, Liimatainen J, Kunnas S (2021). Essential oils and supercritical CO2 extracts of Arctic Angelica (Angelica archangelica L.), marsh Labrador tea (Rhododendron tomentosum) and common tansy (Tanacetum vulgare)—chemical compositions and antimicrobial activities. Molecules, 26(23), 7121
• [44] Lai PK, Roy J (2004). Antimicrobial and chemopreventive properties of herbs and spices. Current Medical Chemistry, 11(11), 1451-1460
• [45] Madubunyi II (1995). Antimicrobial activities of the constituents of Garcinia kola seeds. International Journal of Pharmacognosy, 33, 232-237
• [46] Masotti V, Juteau F, Bessiere JM, Viano J (2003). Seasonal and phenological variations of the essential oil from the narrow endemic species Artemisia molinieri and its biological activities. Journal of Agricultural and Food Chemistry, 51, 7115-7121. DOI: 10.1021/jf034621y
• [47] Mbaze LM, Lado JA, Wansi JD, Shiao TC, Chiozem DD, Mesaik MA, Choudhary MI, Lacaille-Dubois MA, Wandji J, Roy R, Sewald N (2009). Oxidative burst inhibitory and cytotoxic amides and lignans from the stem bark of Fagara heitzii (Rutaceae). Phytochemistry, 70(11-12), 1442-1447. DOI: 10.1016/j.phytochem.2009.08.007
• [48] Mohamed EAA, Muddathir AM, Osman MA (2020). Antimicrobial activity of Eucalyptus camaldulensis Dehn. plant extracts and essential oils: A review. Industrial Crops and Products, 132, 413-429
• [49] Montanher AB, Zucolotto SM, Schenkel EP, Fröde TS (2008). Evidence of anti-inflammatory effects of Passiflora edulis in an inflammation model. Journal of Ethnopharmacology, 109(2), 281-288
• [50] Morton JF (1987). Fruits of Warm Climates. Wipf and Stock Publishers. pp. 115-121. ISBN 978-0-9653360-7-9
• [51] Moudi M, Go R, Yong Seok Yien C, Nazre M (2013). Vinca alkaloids. International Journal of Preventive Medicine, 4(11), 1231-1235
• [52] Nandhini P, Kumar P, Mickymaray S, Alothaim AS, Somasundaram J, Rajan M (2022). Recent developments in Methicillin-Resistant Staphylococcus aureus (MRSA) treatment: A review. Antibiotics, 11(5), 606
• [53] Pazyar N, Yaghoobi R, Ghassemi MR, Kazerouni A, Rafeie E, Jamshydian N (2013). Jojoba in dermatology: A succinct review. Giornale Italiano di Dermatologia e Venereologia, 148(6), 687-691
• [54] Pelczar, M. J., Chan, E. C. S., & Krieg, N. R. (1988). Control of microorganisms, the control of microorganisms by physical agents. In Microbiology. New York: McGraw-Hill International. (pp. 469-509)
• [55] Pichersky, E., Noel, J. P., & Dudareva, N. (2006). Biosynthesis of plant volatiles: Nature's diversity and ingenuity. Science, 311(5762), 808-811
• [56] Puneet, G., Aditi, S., Vinayak, K., Anant, G. N., Samir, D., & Nikhil, M. (2013). Camellia sinensis (tea): Implications and role in preventing dental decay. Pharmacognosy Reviews, 7(14), 152-156. DOI: 10.4103/0973-7847.120515
• [57] Richard, D., Kefi, K., Barbe, U., Bausero, P., & Visioli, F. (2008). Polyunsaturated fatty acids as antioxidants. Pharmacological Research, 57(6), 451-455. DOI: 10.1016/j.phrs.2008.05.002
• [58] Saeidnia, S., Gohari, A. R., Mokhber-Dezfuli, N., & Kiuchi, F. (2011). A review on phytochemistry and medicinal properties of the genus Achillea. Daru, 19(3), 173-186.
• [59] Sarker, S. D., & Nahar, L. (2004). Natural medicine: the genus Angelica. Current Medicinal Chemistry, 11(11), 1479-1500
• [60] Seigler, D. S. (1995). Plant Secondary Metabolism. New York: Springer Science+Business Media. ISBN: 978-1-4613-7228-8; ISBN: 978-1-4615-4913-0 (eBook). DOI: 10.1007/978-1-4615-4913-0
• [61] Serafini, M., Peluso, I., & Raguzzini, A. (2010). Flavonoids as anti-inflammatory agents. Proceedings of the Nutrition Society, 69(3), 273-278. DOI: 10.1017/S002966511000162X
• [62] Sharma, P. R., Shanmugavel, M., Saxena, A. K., & Qazi, G. N. (2008). Induction of apoptosis by a synergistic lignan composition from Cedrus deodara in human cancer cells. Phytotherapy Research, 22(12), 1587-1594. DOI: 10.1002/ptr.2511
• [63] Silén, H., Salih, E. Y., Mgbeahuruike, E. E., & Fyhrqvist, P. (2023). Ethnopharmacology, Antimicrobial Potency, and Phytochemistry of African Combretum and Pteleopsis Species (Combretaceae): A Review. Antibiotics, 12(2), 264
• [64] Sofowa, L. A. (2008). Medicinal Plants and Traditional Medicine in Africa (2nd edition). Spectrum Books Ltd. Ibadan.
• [65] Soni, N., & Singh, V. K. (2019). Traditional, nutraceutical and pharmacological approaches of Tamarindus indica (Imli). European Journal of Biological Research, 9(3), 141-154
• [66] Spiller, F., Alves, M. K., Vieira, S. M., Carvalho, T. A., Leite, C. E., Lunardelli, A., Poloni, J. A., Cunha, F. Q., & de Oliveira, J. R. (2008). Anti-inflammatory effects of red pepper (Capsicum baccatum) on carrageenan- and antigen-induced inflammation. The Journal of Pharmacy and Pharmacology, 60(4), 473-478. DOI: 10.1211/jpp.60.4.0010
• [67] Sravanthi, T., Waghray, K., & Rao, D. S. (2017). Phytochemical screening and anti-microbial and anti-oxidant studies of dehydrated tender tamarind (Tamarindus indica) leaves. International Journal of Food Science and Nutrition, 2(1), 62–63
• [68] Subramaniam, S., Fahy, E., Gupta, S., Sud, M., Byrnes, R. W., Cotter, D., Dinasarapu, A. R., & Maurya, M. R. (2011). Bioinformatics and systems biology of the lipidome. Chemical Reviews, 111(10), 6452-6490. DOI: 10.1021/cr200295k
• [69] Subramanya, J. K., & Muttagi, S. (2012). In vitro color change of three dental veneering resins in tea, coffee, and tamarind extracts. Journal of Dentistry Tehran University of Medical Sciences, 8(3), 138-145
• [70] Susana, J., Beatriz, G. M., Fabiana, C. M., Maria, A. D., & Moacir, G. P. (2011). Antifungal activity of five species of Polygala. Brazilian Journal of Microbiology, 42(3), 1065-1075. DOI: 10.1590/S1517-838220110003000027
• [71] Tadeusz, A. (2015). Alkaloids: Chemistry, Biology, Ecology, and Applications (2nd ed.). Amsterdam, Netherlands: Elsevier. ISBN: 13: 978-0444594334
• [72] Tamale, E., Jones, N., & Pswarayi-Riddihough, I. (August 1995). Technologies Related to Participatory Forestry in Tropical and Subtropical Countries. World Bank Publications. ISBN 978-0-8213-3399-0.
• [73] Tigabu, A., & Getaneh, A. L. E. M. (2021). Staphylococcus aureus, ESKAPE bacteria challenging current health care and community settings: a literature review. Clinical Laboratory, 67(7), 10-7754
• [74] Urish, K. L., & Cassat, J. E. (2020). Staphylococcus aureus osteomyelitis: bone, bugs, and surgery. Infection and Immunity, 88(7), e00932-19.
• [75] Vallekobia, A., Kostalova, D., & Sochorova, R. (2001). Isoquinone alkaloid from Ahonia aquifoliusm stem bark is active against Malassezia species. Folia Microbiology, 46, 107-111.
• [76] Wilhelm, R., Hermann, M. Bolt. (2012). Esters, Organic. Ullmann's Encyclopedia of Industrial Chemistry, 2, 565. DOI: 10.1002/14356007.a09_565.
• [77] Xu, L., Wu, Y., Zhao, X., & Zhang, W. (2015). The study on biological and pharmacological activity of coumarins. In Asia-Pacific Energy Equipment Engineering Research Conference (pp. 135-138).
• [78] Yadav, R. N. S., & Munin, A. (2011). Phytochemical analysis of some medicinal plants. Journal of Phytology, 3(12), 10-14
• [79] Yadima, S. G., Muhammad, M., & Usman, B. (2017). Phytochemical screening and antibacterial activities of aqueous extracts of Diospyros mespiliformis, Mitragyna inermis, Piliostigma reticulatum and Tamarindus indica in Northern Nigeria. Arid Zone Journal of Engineering, Technology, and Environment, 13(3), 411-419.
• [80] Yi, Z., Wang, Z., Li, H., & Liu, M. (2004). Inhibitory effect of tellimagrandin I on chemically induced differentiation of human leukemia K562 cells. Toxicology Letters, 147(2), 109-119.
• [81] Zbigniew, S., Beata, Ż., Kamil, J., Roman, F., Barbara, K., & Andrzej, D. (2014). Antimicrobial and antiradical activity of extracts obtained from leaves of three species of the genus Pyrus. Microbial Drug Resistance, 20(4), 337-343. DOI: 10.1089/mdr.2013.0155

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