SYNTHESIS AND CHARACTERIZATION OF COMPOSITES BASED ON HYDROXYAPATITE NANOPARTICLES AND CHITOSAN EXTRACTED FROM SHELLS OF THE FRESHWATER CRAB Potamon algeriense

• Authors: Mohammed Lakrat , Soufiane Fadlaoui , Mohamed Aaddouz , Ouahid El Asri , Mohammed Melhaoui , Mejdoubi El Miloud
• Languages of publication: EN

Abstracts

EN

Nanocrystalline hydroxyapatite (n-HAp), which has low crystallinity, has attracted great attention due to its similarity to the inorganic part of human bone. Therefore, many studies have focused on creating new formulations combining n-HAp with some biopolymers, such as chitosan, in order to imitate biological bone tissue. The importance of chitosan and its derivatives in biomedical applications has grown significantly in the last three decades due to its biodegradability and renewable source. Besides, chitosan and its derivatives present excellent biocompatibility and biofunctionality, which make them promising materials in bone tissue engineering. In the present study, the chitosan was, first, extracted from the shell of the freshwater crab species Potamon algeriense following demineralization, deproteinization, decolouration (raw chitin) and deacetylation (chitosan) steps. Then, a novel composite based on n-HAp and extracted chitosan (CTS) with varying chitosan contents, from 5% to 20% (w/w), was synthesized and characterized for potential application in tissue regeneration. The obtained composites were characterized using X-ray diffraction, Fourier transform infrared spectroscopy and thermogravimetric analysis. The precipitated n-HAp/CTS nanocomposites similar to natural bone are promising composites for bone tissue engineering applications.

Keywords

EN

Potamon algeriense; bone tissue engineering; chitosan; freshwater crab; nanocomposites; nanocrystalline hydroxyapatite

Discipline

• CHEMISTRY: CHEMISTRY

• Publisher: Sieć Badawcza Łukasiewicz - Polskie Towarzystwo Chitynowe
• Journal: Progress on Chemistry and Application of Chitin and its Derivatives
• Year: 2020
• Volume: 25
• Pages: 132 - 142

Contributors

author

Mohammed Lakrat

• Laboratory of Mineral Solid and Analytical Chemistry (LMSAC), Department of Chemistry, Faculty of Sciences, Mohamed 1st University

author

Soufiane Fadlaoui

• Laboratory of Water, Environment, and Sustainable Development, Mohamed First University,

author

Mohamed Aaddouz

• Laboratory of Mineral Solid and Analytical Chemistry (LMSAC), Department of Chemistry, Faculty of Sciences, Mohamed 1st University,

author

Ouahid El Asri

• Microbiology and Biotechnology Laboratory, Faculty of Sciences, Ibn Zohr University,

author

Mohammed Melhaoui

• Laboratory of Water, Environment, and Sustainable Development, Mohamed First University,

author

Mejdoubi El Miloud

• Laboratory of Mineral Solid and Analytical Chemistry (LMSAC), Department of Chemistry, Faculty of Sciences, Mohamed 1st University,

References

• [1] Cunniffe GM, O’Brien FJ, Partap S, Levingstone TJ, Stanton KT, Dickson GR; (2010) The synthesis and characterization of nanophase hydroxyapatite using a novel dispersant-aided precipitation method, J. Biomed. Mater Res – Part A. 95 1142–1149. https://doi.org/10.1002/jbm.a.32931.
• [2] Dorozhkin SV; (2010) Nanosized and nanocrystalline calcium orthophosphates, Acta Biomater. 6 715–734. https://doi.org/10.1016/J.ACTBIO.2009.10.031. [3] Kantharia N, Naik S, Apte S, Kheur M, Kheur S, Kale B; (2014) Nano-hydroxyapatite and its contemporary applications. J Dent Res Sci Dev. 1 15. https://doi.org/10.4103/2348-3407.126135.
• [4] Wei G, Ma PX; (2004) Structure and properties of nano-hydroxyapatite/polymer composite scaffolds for bone tissue engineering. Biomaterials. 25 4749–4757. https://doi.org/10.1016/j.biomaterials.2003.12.005.
• [5] Sudarshan NR, Hoover DG, Knorr D; (1992) Antibacterial Action of Chitosan. Food Biotechnol. 6 257–272. https://doi.org/10.1080/08905439209549838.
• [6] Rashidova SS, Milusheva RY, Voropaeva NL, Pulatova SR, Nikonovich GV, Ruban IN; (2004) Isolation of chitin from a variety of raw materials, modification of the material, and interaction its derivatives with metal ions. Chromatographia. 59 783–786. https://doi.org/10.1365/s10337-004-0290-0.
• [7] Ravi Kumar MNV; (2000) A review of chitin and chitosan applications. React Funct Polym. 46 1–27. https://doi.org/10.1016/S1381-5148(00)00038-9.
• [8] Wysokowski M, Szalaty TJ, Jesionowski T, Motylenko M, Rafaja D, Koltsov I, Stöcker H, Bazhenov VV, Ehrlich H, Stelling AL, Beyer J, Heitmann J, Petovic S, Đurović M; (2017) Extreme biomimetic approach for synthesis of nanocrystalline chitin-(Ti,Zr)O2 multiphase composites. Mater Chem Phys. 188 115–124. https://doi.org/10.1016/j.matchemphys.2016.12.038.
• [9] Wang W, Cao N, Dong J, Boukherroub R, Liu W, Li Y, Cong H; (2019) Chitosan/hydroxyapatite modified carbon/carbon composites: synthesis, characterization and: in vitro biocompatibility evaluation. RSC Adv. 9 23362–23372. https://doi.org/10.1039/c8ra10396h.
• [10] Sekiguchi S, Miura Y, Kaneko H, Nishimura SI, Nishi N, Iwase M, Tokura S; (1994) Molecular Weight Dependency of Antimicrobial Activity by Chitosan Oligomers. Food Hydrocoll. Springer US,: pp. 71–76. https://doi.org/10.1007/978-1-4615-2486-1_6.
• [11] Zhao D, Yu S, Sun B, Gao S, Guo S, Zhao K; (2018) Biomedical applications of chitosan and its derivative nanoparticles, Polymers (Basel). https://doi.org/10.3390/polym10040462.
• [12] Kim IY, Seo SJ, Moon HS, Yoo MK, Park IY, Kim BC, Cho CS; (2008) Chitosan and its derivatives for tissue engineering applications. Biotechnol Adv. 26 1–21. https://doi.org/10.1016j.biotechadv.2007.07.009.
• [13] Pighinelli L, Kucharska M; (2013) Chitosan-hydroxyapatite composites. Carbohydr Polym. 256–262. https://doi.org/10.1016/j.carbpol.2012.06.004.
• [14] Boudemagh D, Venturini P, Fleutot S, Cleymand F; (2019) Elaboration of hydroxyapatite nanoparticles and chitosan/hydroxyapatite composites: a present status. Polym Bull. 76 2621–2653. https://doi.org/10.1007/s00289-018-2483-y.
• [15] Fadlaoui S, El Asri O, Mohammed L, Sihame A, Omari A, Melhaoui M; (2019) Isolation and characterization of chitin from shells of the freshwater crab potamon algeriense. Prog Chem Appl Chitin Its Deriv. 24 23–35. https://doi.org/10.15259/PCACD.24.002.
• [16] No HK, Lee MY; (1995) Isolation of chitin from crab shell waste. J Kor Soc Food Nutri (Korea Republic).
• [17] Cho HR, Chang DS, Lee WD, Jeong ET, Lee EW; (1998) Utilization of chitosan hydrolysate as a natural food preservative for fish meat paste products. Kor J Food Sci Tech. 30(4), 817–822.
• [18] No HK, Lee KS, Meyers SP; (2000) Correlation between physicochemical characteristics and binding capacities of chitosan products. J Food Sci. 65 1134-1137. https://doi.org/10.1111j.1365-2621.2000.tb10252.x.
• [19] Mondal S, Pal U, Dey A; (2016) Natural origin hydroxyapatite scaffold as potential bone tissue engineering substitute. Ceram Int. 42 18338–18346. https://doi.org/10.1016/J.CERAMINT.2016.08.165.
• [20] Duarte ML, Ferreira MC, Marvão MR, Rocha J; (2002) An optimised method to determine the degree of acetylation of chitin and chitosan by FTIR spectroscopy, Int. J. Biol. Macromol. https://doi.org/10.1016/S0141-8130(02)00039-9.
• [21] Rahman MA, Halfar J; (2014) First evidence of chitin in calcified coralline algae: New insights into the calcification process of Clathromorphum compactum, Sci. Rep. 1–11. https://doi.org/10.1038/srep06162.
• [22] Erdogan S, Kaya M, Akata I; (2017) Chitin extraction and chitosan production from cell wall of two mushroom species (Lactarius vellereus and Phyllophora ribis), in: AIP Conf. Proc., American Institute of Physics Inc. https://doi.org/10.1063/1.4975427.
• [23] Kaya M, Sargin I, Tozak KO, Baran T, Erdogan S, Sezen G; (2013) Chitin extraction and characterization from Daphnia magna resting eggs, Int. J. Biol. Macromol. https://doi.org/10.1016/j.ijbiomac.2013.08.016.
• [24] Dwiasih P, Hidayat N, Kurniawan R; (2019) Sonochemical Synthesis of Nano-Hydroxyapatite/Chitosan Biomaterial Composite from Shellfish and Their Characterizations, IOP Conf. Ser. Mater. Sci. Eng. https://doi.org/10.1088/1757-899X/515/1/012050.
• [25] Scherrer P; (1918) Bestimmung der Größe und der inneren Struktur von Kolloidteilchen mittels Röntgenstrahlen, Nachrichten von Der Gesellschaft Der Wissenschaften Zu Göttingen, Math. Klasse. 98–100. https://eudml.org/doc/59018 (accessed October 13, 2018).
• [26] Ebrahimpour A, Johnsson M, Richardson CF, Nancollas GH; (1993) The characterization of hydroxyapatite preparations, J. Colloid Interface Sci. https://doi.org/10.1006/jcis.1993.1307.
• [27] Rey C, Shimizu M, Collins B, Glimcher MJ; (1990) Resolution-enhanced Fourier transform infrared spectroscopy study of the environment of phosphate ions in the early deposits of a solid phase of calcium-phosphate in bone and enamel, and their evolution with age. I: Investigations in the upsilon 4 PO4 domain. Calcif Tissue Int. 46 384–94. http://www.ncbi.nlm.nih.gov/pubmed/2364326 (accessed October 2, 2018).
• [28] Kong L, Gao Y, Cao W, Gong Y, Zhao N, Zhang X; (2005) Preparation and characterization of nano-hydroxyapatite/chitosan composite scaffolds. J Biomed Mater Res - Part A. 75 275–282. https://doi.org/10.1002/jbm.a.30414.
• [29] Manjubala I, Scheler S, Bössert J, Jandt KD; (2006) Mineralisation of chitosan scaffolds with nano-apatite formation by double diffusion technique, Acta Biomater. https://doi.org/10.1016j.actbio.2005.09.007.
• [30] Dorozhkin SV; (2015) Calcium orthophosphate deposits: Preparation, properties and biomedical applications. Mater Sci Eng C Mater Biol Appl. 55 272–326. https://doi.org/10.1016/j.msec.2015.05.033.
• [31] Danilchenko SN; (2009) Chitosan–hydroxyapatite composite biomaterials made by a one step co-precipitation method: preparation, characterization and in vivo tests. J Biol Phys Chem. 9 119–126. https://doi.org/10.4024/22da09a.jbpc.09.03.
• [32] Thein-Han WW, Misra RDK; (2009) Biomimetic chitosan-nanohydroxyapatite composite scaffolds for bone tissue engineering. Acta Biomater. 5 1182–1197. https://doi.org/10.1016/j.actbio.2008.11.025.
• [33] Boulila S; (2016) Comportement de verres composites poreux : assimilation osseuse, explorations physiologiques et physico-chimiques, Http://Www.Theses.Fr. http://www.theses.fr/2016REN1S105 accessed February 5, 2019).
• [34] Chen J, Wang Z, Wen Z, Yang S, Wang J, Zhang Q; (2015) Controllable self-assembly of mesoporous hydroxyapatite. Colloids Surfaces B Biointerfaces. 127 47–53. https://doi.org/10.1016j.colsurfb.2014.12.055.

• Document Type: article