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2018 | 105 | 145-156
Article title

Serodiagnosis of Toxoplasmosis using Lateral Flow Chromatographic Immunoassay among Animals and Humans in Sunsari District of Nepal

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EN
Toxoplasmosis is a cosmopolitan parasitic zoonosis, infecting human and other warm blooded animals worldwide. This disease has economic importance in regard to animal reproduction, and it leads to abortions and neonatal complications in humans. This study was carried out to determine the seroprevalence of Toxoplasma gondii in sheep, cattle, cats and human in Inaruwa and surrounding areas of Sunsari district, Nepal. Altogether 336 blood samples, of which 50 from sheep, 92 from cattle, 44 from cats and 150 from human were collected and tested immediately using lateral flow chromatographic immunoassay (Toxo IgG/IgM Combo Rapid test®). Associated biometric information such as age, sex, pregnancy status, occupation, association with cat was recorded and analyzed to determine the association of risk factors with the disease. Data were analyzed using R 3.2.2 (The R foundation for Statistical Computing, 2015). Seroprevalence of toxoplasmosis was detected 12.00% (95% CI: 4.53- 24.31%) in sheep, 8.70% (95% CI: 3.83- 16.42%) in cattle, 36.36% (95%CI: 22.41- 52.23%) in cats and 12.67% (95% CI: 7.80- 19.07%) in human. In case of human, 31 to 45 years age group were found more susceptible to toxoplasmosis (21.74%, OR: 6.4) in comparison to 21 to 30 years (10.0%) and up to 20 years (4.17%) age groups. Toxoplasmosis was found highly significantly associated with abortion (58.33%, OR= 15.4, P=0.0001) in human in the tested individuals. Regarding occupation, 20.83% butchers were seropositive followed by farmers (15.52%), housewives (10.0%) and diagnostic lab technicians (8.0%). Female and higher age group showed high prevalence of toxoplasmosis in all studied species. Therefore, this assay is a useful method for the serological screening of toxoplasmosis in different animals and humans.
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Volume
105
Pages
145-156
Physical description
Contributors
  • Agricultural Research Station, Pakhribas, Nepal Agricultural Research Council, Nepal
  • Department of Parasitology, Bangladesh Agricultural University, Mymensingh, Bangladesh
  • Department of Medicine, Bangladesh Agricultural University, Mymensingh, Bangladesh
References
  • [1] E.A. Innes, A Brief History and Overview of Toxoplasma gondii. Zoonoses and Public Health, 57 (2010) 1-7.
  • [2] K.S. Pereira, R.M. Franco, D.A. Leal, Transmission of toxoplasmosis (Toxoplasma gondii) by foods. Advances in Food and Nutrition Research 60 (2010) 1-19.
  • [3] J. Montoya, O. Liesenfeld, Toxoplasmosis. Lancet 363 (2004) 1965-1976.
  • [4] J.P. Dubey, C.P. Beattie, Toxoplasmosis of Animals and Man. Boca Raton, Florida: CRC Press (1998) 220.
  • [5] J.S. Remington, R. McLeod, P. Thulliez, G. Desmonts, Toxoplasmosis. Infectious diseases of the fetus and newborn infant, 5th ed. W. B. Saunders, Philadelphia (2001) 205-346.
  • [6] J.P. Dubey, D.E. Hill, D.W. Remington, O.C. Kwox, S. Sue, High prevalence and genotypes of Toxoplasma gondii isolated from organic pigs in northern USA. Veterinary Parasitology 188 (1) (2013) 14-18.
  • [7] J.P. Dubey, Toxoplasmosis – a waterborne zoonosis. Veterinary Parasitology 126 (2004) 57-72.
  • [8] W.C. Lake, W. Khachaeramb, S. Koizumic, S. Maruyama, Seroprevalence of Toxoplasma gondii infection in domestic goats in Satun Province, Thailand. Veterinary Parasitology 127(2002) 17-22.
  • [9] J.P. Dubey, Toxoplasmosis of Animals and Humans. 2nd edition. Boca Raton, Florida: CRC Press (2010) 313.
  • [10] E.G. Zewdu, A. Hailu, T.S. Tessema, K.T. Desta, G. Medhin, M. Vitale, V. Di Marco, E. Cox, P. Dorny, Seroepidemiology of Toxoplasma gondii infection in women of child-bearing age in central Ethiopia. BMC Infectious Diseases 13 (2013) 101.
  • [11] D. Hill, J.P. Dubey, Toxoplasma gondii: transmission, diagnosis and prevention. Clinical Microbiology and Infectious Diseases 8 (2002) 634-640.
  • [12] M. Pal, Zoonoses. 2nd edition (2007). Satyam Publishers, Jaipur, India.
  • [13] S.K. Rai, H. Shibata, K. Sumi, K. Kubota, K. Hirai, A. Matsuoka, T.T. Kubo, S.R. Basnet, H.G. Shrestha, R.C. Mahajan, Seroepidemiological study of toxoplasmosis in two different geographical areas in Nepal. Southeast Asian Journal Tropical Medical Public Health 25(3) (1994) 479-84.
  • [14] M.P. Upadhayay, S.K. Rai, R.K. Bhandari, P. Bista, Serological study of Toxoplasma gondii infection in Kathmandu, Nepal. Journal of Institute Medicine 2 (1989) 211-5.
  • [15] S. Jaiswal, T. Pokhrel, S. Sharma, S.R. Ranabhat, D.K. Yadav, B. Koirala, Seropositivity Rates of Toxoplasmosis and Syphilis in Pregnant Women Visiting Western Regional Hospital, Nepal. International Journal of Health Sciences & Research 230(4) (2014) 9230-42.
  • [16] I.R. Tizard, S.S. Chauhan, C.H. Lai, The prevalence and epidemiology of toxoplasmosis in Ontario. Journal of Hygiene Cambodia 78 (1977) 275-82.
  • [17] H.A. Feldman, Epidemiology of Toxoplasma infection. Epidemiology Review 4 (1982) 204-13.
  • [18] H. Suzuki, Y. Yamamoto, K. Matsumoto, A serological study of Toxoplasma infection in Nagasaki by ELISA. Japanese Journal of Parasitology 36 (1987) 118-24.
  • [19] A. Driscoll Carlos, J. Clutton Brock, C. Kitchener Andrew, O.S.J. Brien, The Evolution of House Cats. Scientific American 2009. New York: Nature Pubg. Group. Retrieved 26 August 2009.
  • [20] R.P. Sah, M.H. Talukder, M.B. Hossain, Toxoplasmosis in cats and its zoonotic potential at Bangladesh Agricultural University campus. 3rd International Exhibition on Dairy, Aqua and Pet (IEDAP) held at BICC Dhaka, Bangladesh (2016) 300-303.
  • [21] C. Giannoulis, B. Zournatzi, A. Giomisi, E. Diza, I. Tzafettas, Toxoplasmosis during pregnancy: a case report and review of literature. Hippokratia 12(3) (2008) 139-143.
  • [22] M. Amany, A.M. Merwad, Epidemiology and molecular detection of zoonotic Toxoplasma gondii in cat feces and seroprevalence of anti-Toxoplasma gondii antibodies in pregnant women and sheep. Life Science Journal 9 (2012) 133-146.
  • [23] B. Hossain, Serodiagnosis of toxoplasmosis in human and animals using lateral flow chromatographic assay. MS Thesis (2016). Department of Parasitology, BAU, Bangladesh.
  • [24] M.A. Samad, B.C. Dey, N.S. Chowdhury, S. Akhtar, Sero-epidemiological studies on Toxoplasma gondii infection in man and animals in Bangladesh. Southeast Asian Journal of Tropical Medicine and Public Health 28(2) (1997) 339-343.
  • [25] M.A. Samad, K.B. Rahman, A.K. Halder, Seroprevalence of Toxoplasma gondii in domestic ruminants in Bangladesh. Veterinary Parasitology 47(1-2) (1993) 157-9.
  • [26] Ashrafunnessa, S. Khatun, M.N. Islam, T. Huq, Seroprevalence of toxoplasma antibodies among the antenatal population in Bangladesh. Journal of Obstetrics and Gynaecology Research 24(2) (1998) 115-9.
  • [27] S.K. Rai, K. Yano, T.T. Kubo, Immuno-serological study of human toxoplasmosis in Nepal. Proceeding Third Congress Asia Pacific Societies of Pathologists and Eleventh National Convention, Bangladesh Society of Pathologists (1993) 98.
  • [28] Y.Y. Jafar, J. Nabi, M.J. Sameri, and N. Noorshahi, Seroprevalence of Anti-Toxoplasma gondii Antibodies Among pregnant woman in South Khuzestan, Iran. Jundishapur Journal of Microbiology 7(5) (2014) e9998.
  • [29] U.H. Margia, Seroprevalence of toxoplasmosis in women of Mymensingh and Rangpur districts. MS Thesis (2015). Department of Parasitology, BAU, Bangladesh.
  • [30] S. Lamaj, G. Dhamo, I. Dova, Epidemiology of Toxoplasma gondii infection of cats in southwest of Albania. Journal of International Academic Research for Multidisciplinary 4(2) (2016) 76-81.
  • [31] A.C.B.T. Nunes, E.M.V. Silva, J.A. Oliveira, E.M. Yamasaki, P.C.P. Kim, J.C. Almeida, K.B. Nunes, R.A. Mota, Application of different techniques to detect Toxoplasma gondii in slaughtered sheep for human consumption. Brazil Journal of Veterinary Parasitology, Jaboticabal 24(4) (2015) 416-421.
  • [32] H. Azizi, B. Shiran, A.B. Boroujeni, M. Jafari, Molecular survey of Toxoplasma gondii in sheep, cattle and meat products in Chaharmahal va Bakhtiari Province, southwest of Iran. Iranian Journal of Parasitology 9(3) (2014) 429-434.
  • [33] J.P. Dubey, M.R. Lappin, Toxoplasmosis and neosporosis. In: Greene CE, editor. Infectious diseases of the dog and cat. 2nd edition. Philadelphia: WB Saunders (1998) 493-509.
  • [34] A.C.A.V. Carneiro, M. Carneiro, A.M.G. Gouveia, A.S. Guimaraes, A.P.R. Marques, L.S.Vilas-Boas, R.W.A. Vitor, Seroprevalence and risk factors of caprine toxoplasmosis in Minas Gerais, Brazil. Veterinary Parasitology 160 (2009) 225-229.
  • [35] M. Ramzan, M. Akhtar, F. Muhammad, I. Hussain, E. Hiszczynska‐Sawicka, A.U. Haq, M.S. Mahmood, M.A. Hafeez, Seroprevalence of Toxoplasma gondii in sheep and goats in Rahim Yar Khan (Punjab), Pakistan. Tropical Animal Health and Production 41 (2009) 1225-1229.
  • [36] L. Halos, A. Thebault, D. Aubert, M. Thomas, C. Perret, R. Geers, A. Alliot, S. Escotte-Binet, D. Ajzenberg, M.L. Darde, B. Durand, P. Boireau, I. Villena, An innovative survey underlining the significant level of contamination by Toxoplasma gondii of ovine meat consumed in France. International Journal for Parasitology 40 (2010) 193-200.
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bwmeta1.element.psjd-b70c2ded-461a-46de-8c48-7905acbabc29
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