Chemical constituents and cytotoxicity for Peliosanthes micrantha rhizomes

• Authors: Do Ngoc Thuy , Le Ngoc Hung , Nguyen Thi Huong , Nguyen Manh Son , Ta Thi Thao , Do Truong Tu
• Languages of publication: EN

Abstracts

EN

The medicinal plant Peliosanthes micrantha of Vietnam has been researched recently. This study explored water extracts of ethanol-extracted P. micrantha rhizome. P. micrantha rhizome was first extracted by ethanol (PM-R-ET). Then, sequential water extract of ethanol-extracted P. micrantha rhizome was carried out (PM-R-ET-W), analyzed by Ultra High-Performance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry (QTOF-MS), and Gel Permeation Chromatography analysis (GPC). Its cytotoxicity was evaluated against five cancer cell lines using MTT assay. In the PM-R-ET-W extract, QTOF-MS tentatively assigned five known compounds, pumilaside A, pumilaside C, β–sitosterol, glycoside J-1, and peliosanthoside B. GPC showed one structurally-unresolved high molecular compound with MW 16.293 Da enriched to 19.3% w/w. PM-R-ET-W cytotoxicity values were moderate against all five MCF-7, Hela, HT-29, HepG2, and A549 cancer cells. The QTOF-MS-identified known compounds in the PM-R-ET-W extract should be later isolated for further biological assays. The GPC-analyzed high molecular compound of 16.293 Da quantified at 19.3 % w/w should be structurally determined. All the tested cell lines’ cytotoxicity values of the PM-R-ET-W were moderate. Six identified compounds should be isolated and tested for their cytotoxicity.

Keywords

EN

Peliosanthes micrantha; UHPLC-QTOF; cancer cell lines; cytotoxicity; water extract

Discipline

• BIOCHEMISTRY: BIOCHEMISTRY

• Publisher: Scientific Publishing House „DARWIN”
• Journal: World News of Natural Sciences
• Year: 2025
• Volume: 63
• Issue: 2
• Pages: 356-368

Contributors

author

Do Ngoc Thuy

• Centre for High Technology Research and Development, Vietnam Academy of Science and Technology, Hoang Quoc Viet 18, 10000, Hanoi, Vietnam

author

Le Ngoc Hung

• Centre for High Technology Research and Development, Vietnam Academy of Science and Technology, Hoang Quoc Viet 18, 10000, Hanoi, Vietnam

author

Nguyen Thi Huong

• Centre for High Technology Research and Development, Vietnam Academy of Science and Technology, Hoang Quoc Viet 18, 10000, Hanoi, Vietnam

author

Nguyen Manh Son

• Department of Chemistry, University of Science, Vietnam National University - Hanoi, No 19 Le Thanh Tong, 10000, Ha Noi, Vietnam

author

Ta Thi Thao

• Department of Chemistry, University of Science, Vietnam National University - Hanoi, No 19 Le Thanh Tong, 10000, Ha Noi, Vietnam

author

Do Truong Tu

• Ho Chi Minh City Space Technology Application Center, Vietnam National Space Center, No. 1 Mac Dinh Chi, Saigon Ward, 700000, Ho Chi Minh City, Vietnam

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• Document Type: article