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2013 | 18 | 18 | 67-76
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The pyruvate kinase isoenzyme M2 originating from the nucleoplasm and cytoplasm of tumor cells, with its highest affinity to the 2-phosphoenolpyruvate (2-PEP) and sensitivity to L-cysteine, contributes to an increased generation of energy as ATP, necessary for tumor cell proliferation.
In the presence of L-cysteine, the isoenzyme M2 PK demonstrates the activity of histone kinase, transferring the phosphoryl group from 2-PEP to the ε-amine residue of the H1 histone lysine.
Oligochitosans induce expression of the inducible nitric oxide synthase gene (iNOS), what results in an increased synthesis of nitric oxide, which reacts with L-cysteine and produces L-S-nitrosocysteine. Lack of L-cysteine contributes to inhibition of kinase activity of the H1 histone, an M2 PK isoenzyme. Decreased phosphorylation of the H1 histone contributes to inhibition of EAT cell proliferation.
No effect on proliferation of normal cells that include the PK M1 isoenzyme has been observed in the presence of oligochitosans.
Physical description
  • Collegium Medicum, Jagiellonian University
  • Institute of Biopolymers and Chemical Fibers
  • Collegium Medicum, Jagiellonian University
  • Institute of Biopolymers and Chemical Fibers
  • 1. Kass G.E., Orenius S.: (1999) Calcium signaling and cytotoxicity. Environ. Health Persp. 107, 25-35.
  • 2. Kornberg R.D., Lorch Y.: (1999) Twenty-five years of the nucleosome, fundamental particle of the eukaryote chromosome. Cell 98;285-29.
  • 3. Farkas G., Leibovitch F., Elgin S.C.: (2000) Chromatin organization and transcriptional control of gene expression in Drosophila. Gene 253; 117-136.
  • 4. Müller C., Leuttz A.: (2001) Chromatin remodeling in development and diferentiation. Curr Opin Genet Dev 11, 167-174.
  • 5. Sudarsanam P., Winston F.: (2000) The Swi/Snf family nucleosome – remodeling complexes and transcriptional control. Trends Genet 16, 345-350.
  • 6. Kingston R.E., Narlikar G.J.: (1999) ATP-dependent remodeling and acetylation as regulators of chromatin fluidity. Genes Dev 13, 2339-2352.
  • 7. Cheung P., Allis C.D., Sassone-Corsi P.: (2000) Signaling to chromatin through histone modifications. Cell 103, 263-271.
  • 8. Zhang Y., Reinberg D.: (2001) Transcription regulation by histone methylation: interplay between different covalent modifications of the core histone tails. Genes Dev 15, 2343-2360.
  • 9. Herrera J.E., West K.L., Schiltz R.L., Nakatani Y., Bustin M.: (2000) Histone H1 is a specific repressor of core histone acetylation in chromatin. Mol Cell Biol 20, 523-529.
  • 10. Hill D.A., Imbalzano A.N.: (2000) Human SWI/SNF nucleosome remodeling activity is partially inhibited by linker histone H1. Biochemistry 39, 11649-11656.
  • 11. Misteli T., Gunjan A., Hock R., Bustin M., Brown D.T.: (2000) Dynamic binding of histone H1 to chromatin in living cells. Nature 408, 877-881.
  • 12. Slawson C., Housley M.P., HartG.W.: (2006) O-GlcNAc cycling: How a single sugar post-translational modyfication is changing the way we think about signaling networks. J. Cell Biochem 97, 71-83.
  • 13. Kudlow J.E.: (2006)Post-translational modyfication by O-GlcNAc: another way to change protein function. J. Cell Biochem. 98, 1062-1075.
  • 14. Majumdar G., Wrigh J., Markowitz P., Martinez-Hernandez A., Raghow R., Solomon S.S.: (2004) Insulin stimulates and diabetes inhibits O-linked N-acetylglucosamine transferaze and O-glycosylation of Sp1. Diabetes 53, 3184-3192.
  • 15. Shaw P., Freeman J., Bovey R., Iggo R.: (1996) Regulation of specyfic DNA binding by p53: evidence for a role for O-glycosylation and charged residues at the carboxy-terminus. Oncogene 12, 921-930.
  • 16. Yang W.H., Kin J.E., Nam H.W., Ju J.W., Kim H.S., Kim Y.S., Cho J.W.: (2006) Modyfication of p53 with o-linked N-acetylglucosamine regulates p53 activity and stability. Nat.Cell Biol. 8, 1074-1083.
  • 17. Hiromura M., Choi C.H., Sabourin N.A., Jones H., Bavhvarov D., Usheva A.: (2003) YY1 is regulated by O-linked N-acetylglucosaminylation (O-GlcNAcylation). J. Biol. Chem. 278, 14046-14052.
  • 18. Ahmad I., Hoessli D.C., Walker-Nasir E., Rafik S.M., Shakoori A.R., Din N.: (2006) Oct-2 DNA binding transcription factor: functional consequences of phosphorylation and glycosylation. Nucleic Acids Res. 34, 175-184.
  • 19. Gewinner C., Hart G., Zachara N., Cole R., Beisenherz-Huss C., Groner B., (2004) The coactivator of transcription CREB-binding protein interacts Preferentially with the glycosylated form of Stat %. J.Biol.Chem. 279, 3563-3572.
  • 20. Lamarre-Vincent N., Hsieh-Wilson L.C.: (2003) Dynamic glycosylation of the transcription factor CREB: a potential role in gene regulation> J. Am. Chem. Soc. 125, 6612-6613.
  • 21. Vanderford N.L., Andrali S.A., Ozcan S.: (2006) Glucose induces MafA expression in pancreatic beta cell lines via the hexosamine biosynthetic pathway. J. Biol. Chem. 282, 1577-1584.
  • 22. Zhang F., Su K., Yang X., Bowe D.B., Paterson A.J., Kudlow J.E.: (2003) O-GlcNAc modification is an endogenous inhibitor of the proteasome. Cell 115, 715-725.
  • 23. Muzzarelli R.A.A.: „Chitin”. Pergamon Press, Oxford, 1981.
  • 24. Quan G., Wang T.: (2007) Chitosan nanoparticle as protein delivery carrier-Systematic examination of fabrication conditions for efficient loading and release. Colloids Surf. B: Bioinnterf, 59, 24-34.
  • 25. Struszczyk H.: (1987) Microcrystalline chitosan. I. Properties and preparation. J. Appl. Polym. Sci. 33, 177-187.
  • 26. Tsikas D., Gutzki F.M., Rossan S., Bauer H., Neumann Ch., Dockendorff K., Sandmann J., Frolich J.C.: (1997) Measurement of nitrite and nitrate in biological fluids by gas chromatogra¬phy – mass spectrometry and by the Griess assay: problems with the Griess assay – solutions by gas chromatography – mass spectrometry. Anal. Biochem. 244, 208-220.
  • 27. Tsikas D., Sandmann J., Rossa S., Gutzki F.M., Frolich J.C.: (1999) Investigations of S-transnitrosylation reactions between low- and high-molecular-weight S-nitroso compounds and their thiols by high-performance liquid chromatography and gas chromatography – mass spectrometry. Anal. Biochem. 270, 231-241.
  • 28. Lowry O.H., Rosebrouggh N.J., Farr A.L., Randal R.J.: (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265-273.
  • 29. Bucher I., Pfleiderer G.: (1955) In: Collowick S P, Kaplan N D (eds), Methods in Enzymology, Acad. Press. New York 1, 435-440.
  • 30. Noguchi T., Inoue H., Tanaka T.: (1986) The H1 – and M2 type isoenzymes of rat pyruvate kinase are produced the same gene by alternative RNA splicing. J. Biol. Chem. 261, 13807-13812.
  • 31. Mazurek S.: (2011) Pyruvate kinase type M2: A key regulator of the metabolic budget system in tumor cells. Inter. J. Biochem. Cell Biol. 43, 969-980.
  • 32. Ignacak J., Wiśniewska-Wrona M., Dulińska-Litewka J., Pałka I., Zagajewski J., Niekraszewicz A.: (2012) Effect of oligochitonsans on expression of iNOS gene in Ehrlich ascites tumour in vitro. In: Jaworska M. (ed.) Progress on Chemistry and Application of Chitin and its Derivatives. Polish Chitin Society, Monograph, vol.XVII, pp. 137-144.
  • 33. Ignacak J., Wiśniewska-Wrona M., Pałka I., Zagajewski J., Niekraszewicz A.: (2011) Role of chitosan oligomers in regulation of Ehrlich ascites tumor Wells proliferation in vitro. In: Jaworska M. (ed.) Progress on Chemistry and Application of Chitin and its Derivatives. Polish Chitin Society, Monograph, vol.XVI, pp. 89-98.
  • 34. Jerzmanowski A., Cole R. D.; (1993) Histone H1 in modulation of chromatintranscriptional activity. Acta Biochem. Pol. 40, 17-22.
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