Preferences help
enabled [disable] Abstract
Number of results
2017 | 67 | 2 | 201-218
Article title

Effects of Rhizobium, Nitrogen and Phosphorus Fertilizers on Growth, Nodulation, Yield and Yield Attributes of Soybean at Pawe Northwestern Ethiopia

Title variants
Languages of publication
Owing to the rising costs of chemical fertilizers and the growing environmental concerns, there is an ever increasing interest in the role of soil microorganisms in crop nutrition and soil fertility restoration. A field study was therefore conducted at Pawe Agricultural Research Center during 2010 main cropping season to determine the influence of Bradyrhizobium japonicum inoculation and N and P fertilizers application on nodulation, yield and yield attributes of soybean. Three levels of N (0, 11.5 and 23 kg N ha-1); three levels of P (0, 23 and 46 kg P2O5 ha-1) with two levels of Rhizobium were arranged in RCBD in factorial combinations with three replications. Nodule number, nodule fresh and dry weights, plant height, number of pods and seeds per plant, 100 seeds weight and grain yield responded significantly to the interaction effects of B. japonicum inoculation and application of N and P fertilizers but nodule volume, seed and biomass yields, and harvest index were significantly affected by the main effects of any one or more of the factors and interaction of any two of the factors. The maximum numbers of nodules of 80.26, fresh and dry weights of 3.77 and 0.99 gm./plant respectively; were measured by combined effect of 11.5 kg N/ha, 46 kg P2O5/ha and B. japonicum. Similarly the highest nodule volume of 3.53 and 3.27 ml/plant were measured after applications of 46 kg P2O5/ha with B. japonicum and with 11.5 kg N/ha respectively. The highest plant heights of 79.26 cm followed by 76.94 cm were measured by application of 46 kg P2O5/ha with B. japonicum and 46 kg P2O5/ha itself respectively. Seed yield significantly increased to 11.91 gm /plant and 15.97 gm /plant following application of B. japonicum alone and 46 kg P2O5/ha with B. japonicum respectively. Applied 23 kg/ha brought the highest plant biomass of 27.25 gm/plant. Inoculation with 46 kg P2O5/ha and the combined effects of 11.5 kg N/ha, 46 kg P2O5/ha and B. japonicum resulted 73.93 and 80.66 number of pods per plant respectively. The maximum 100.60 numbers of seeds per plant were counted during inoculation of B. japonicum with 46 kg P2O5/ha. The maximum 100-seed weight of 16.96 gm and grain yield of 3151.88 kg/ha were produced by combined application of 11.5 kg N/ha, 46 kg P2O5/ha and B. japonicum. Each nodule attributes were significantly and positively correlated each other and with each yield and yield attributes. The results showed that growth and yield potential of soybean and an increase N2 fixing can be achieved by using B. japonicum and P fertilizer with reduced level of N as starter fertilizer.
Physical description
  • Horticulture Department, College of Agriculture and Natural Resources, Debremarkos University, Debremarkos, Ethiopia
  • School of Natural Resource Management and Environmental Science, College of Agriculture and Environmental Science Haramaya University, Alemaya, Ethiopia
  • [1] Abbasi, M. K., A. Majeed., A. Sadiq, and S. R. Khan, 2008. Application of Bradyrhizobium japonicum and phosphorus fertilization improved growth, yield and nodulation of soybean in the sub-humid hilly region of Azad Jammu and Kashmir, Pakistan. Pak. J. Plant Prod. Sci. 58, 368-376.
  • [2] Asgelil Dibabe, 2000. Effect of fertilizer on the yield and nodulation pattern of faba bean on a Nitosol of Adet, North Western Ethiopia. Ethio. J. Nat. Res. 2, 237-244.
  • [3] Behailu Kebede, 2006. Land cover, land use changes and agroforestry practices at Pawe resettlement district, northwestern Ethiopia. An MSc Thesis, Hawassa University, Wondo Genet College of Forestry and Natural Resources, Ethiopia.
  • [4] Brady, N. C., 2002. Phosphorus and potassium. In: The nature and properties of soils. Prentice Hall of India, Delhi. 352 p.
  • [5] Bray R.H. and L. T. Kurtz, 1954. Determination of total organic and available forms of phosphorous in soil. J. Soil Sci. 59, 39-45.
  • [6] Bremner, J. M. and C. S. Mulvaney, 1982. Total nitrogen. pp. 595-624. In: A.L Page (eds.). Method of Soil Analysis. Agron. No. 9. Amer. Soc. Agron. Madison, WI, USA.
  • [7] Cheema, Z. A. and A. Ahmad, 2000. Effects of urea on the nitrogen fixing capacity and growth of grain legumes. Int. J. Agric. Biol. 2(4), 388-394.
  • [8] Datsenko, V. K., S. K. Laguta, E. P. Starchenkov, A. F. Antipchuk, and V. N. Rangelova, 1997. Efficiency of legume-rhizobia symbiosis in various soybean varieties and Bradyrhizobium japonicum cultures. Fiziol. Biokhim. Kul’t. Rast. 29, 299-303.
  • [9] Day, P. R.., 1965. Hydrometer method of particle size analysis. pp. 562-563. In: C.A. Black (eds.). Methods of Soil Analysis. Agronomy Part II, No. 9. Amer. Soc. Agron. Madison, Wisconsin, USA.
  • [10] Dubey, S. K., 1998. Response of soybean to biofertilizers with and without nitrogen, phosphorus and potassium on swell-shrink soil. Ind. J. Agron. 43, 546-549.
  • [11] Egamberdiyeva, D., D. Qarshieva, K. Davranov, 2004. The use of Bradyrhizobium to enhance growth and yield of soybean in calcarious soil in Uzbekistan. J. Plant Growth Regul. 23, 54-57.
  • [12] Fatima, Z., M. Zia, M.F. Chaudhary, 2007. Interactive effect of Rhizobium strains and phosphorus on soybean yield, nitrogen fixation and soil fertility. Pak. J. Bot. 39, 255-264.
  • [13] Herridge, D. F., R. J. Roughley and J. Brockwell, 1984. Effects of Rhizobium and soil nitrate on establishment and functioning of the soybean symbiosis in the field. Austr. J. Agric. Res. 35, 146-161.
  • [14] Islam, A. K. M. S., G. Kerven and J. Oweczkin, 1992. Methods of Plant Analysis. ACIAR 904 IBSRAM QC.
  • [15] Jackson, M. L., 1958. Soil Chemical Analysis. Prentice Hall, Inc., Englewood Cliffs, New Jersy. Pp. 183-204.
  • [16] Jalaluddin, M., 2005. Effect of inoculation with vam-fungi and Bradyrhizobium on growth and yield of soybean in Sindh. Pak. J. Bot. 37: 169-173.
  • [17] Kihanda, F. M., 1996. The role of farmyard manure in improvement of maize production in the sub-humid highlands of central Kenya. Ph.D Thesis, Reading University, United Kingdom.
  • [18] Kovats, A., L. Marton and L. Szabo, 1985. Analysis of the relation between humus and pH on the ground of results of soil investigations on farm-scale plots. Plant Prod. 34, 507-512.
  • [19] Laszlo, M. and E. M. Jose, 2001. Effects of Erotalaria juncea L. and Erotalaria spectabilis ROTH on soil fertility and soil conservation in Hungary. Acta Agronomica Óváriensis 43, 1-8.
  • [20] Mahmood, J., Y. A. Abayomi, M. O. Aduloju, 2009. Comparative growth and yield responses of soybean genotypes to phosphorus fertilizer application. Afr. J. Biotech. 8, 1030-1036.
  • [21] Malik, M. A., M. A. Cheema, H. Z. Khan and M. A. Wahid, 2006. Growth and yield response of soybean (Glycine max L.) to seed inoculation and varying phosphorus levels. J. Agric. Res. 44(1), 47-53
  • [22] Moharram, T. M. M., M. S. A. Safwat, M. M. Farghaly, 1994. Effect of inoculation rates and phosphorus fertilization on nitrogen fixation in soybean. Afr. J. Crop Sci. 2, 125-129.
  • [23] Mrkovacki, N., J. Marinkovic, R. Rilmovic, 2008. Effect of n fertilizer application on growth and yield of inoculated soybean. Not. Bot. Hort. Agrobot. Cluj. 36, 48-51.
  • [24] Olivera, M., N. Tejera, C. Iribarne, A. Ocana, C. Lluch, 2004. Growth, nitrogen fixation and ammonium assimilation in common bean (Phaseolus vulgaris). Plant Physiol. 121, 498-505.
  • [25] Plaxton, W. C., 2004. Plant response to stress, biochemical adaptations to phosphate deficiency. Pp. 976-980. In: R. Goodman (ed.). Encyclopedia of Plant and Crop Science. Marcel Dekker, New York.
  • [26] Rathke, G. W., O. Christen and W. Diepenbrock, 2005. Effects of nitrogen source and rate on productivity and quality of winter oilseed rape (Brassica napus L.) grown in different crop rotations. Field Crop Res. 94, 103-113.
  • [27] Ray, J. D., L. G. Heatherly, F. B. Fritschi, 2006. Influence of large amounts of nitrogen applied at planting on non-irrigated and irrigated soybean. Crop Sci. 46, 52-60.
  • [28] Revellin, C., G. Meunier, J. J. Giraud, G. Sommer, P. Wadoux, G. Catroux, 2000. Changes in the physiological and agricultural characteristics of peat-based Bradyrhizobium japonicum inoculants after long term storage. Appl. Microbiol. Biotech. 54, 206-211.
  • [29] Rice, W. A., G. W. Clayton, N. Z. Lupwayi and P. E. Olsen, 2001. Evaluation of coated seeds as a Rhizobium delivery system for field pea. Can. J. Plant Science 81, 248-249.
  • [30] Sahlemedhin Sertsue and Taye Bekele, 2000. Procedures for soil and plant analysis. National Soil Research Center, Ethiopian Agricultural Research Organization, Addis Ababa, Ethiopia. 110 p.
  • [31] Sarr A., B. Diop, R. Peltier, M. Neyra, D. Lesueur, 2005. Effect of rhizobial inoculation methods and host plant provenances on nodulation and growth of Acacia senegal and Acacia nilotica. New Forests 29, 75- 87.
  • [32] SAS (Statistical Analysis System) Institute, 2004. The SAS system for windows, version 9.0. SAS Institute Inc., Cary, NC., USA.
  • [33] Schulze, J., G. Temple, S. J. Temple, H. Beschow and C. P. Vance, 2006. Nitrogen fixation by white lupin under phosphorus deficiency. Ann. Bot. 98, 731-740.
  • [34] Sinclair, T. R. and V. Vadez, 2002. Physiological traits for crop yield improvement in low N and P environments. Plant and Soil. 245, 1-15.
  • [35] Somasegaran and Hoben, 1985. Methods in legume-Rhizobium technology. University of Hawaii Niftal Project and MIRCEM. Department of Agronomy and Soil Science, University of Hawaii.
  • [36] Subba Rao, N. S., 1988. Rhizobium inoculants: Biofertilizer in agriculture and forestry, second ed. Oxford and IBH Publishing Co. Pvt Ltd, New Delhi, India. Pp. 16-76.
  • [37] Tahir, M. M., M. K. Abbasi, N. Rahim, A. Khaliq and M. H. Kazmi, 2009. Effects of Rhizobium inoculation and NP fertilization on growth, yield and nodulation of soybean (Glycine max L.) in the sub-humid hilly region of Rawalakot Azad Jammu and Kashmir, Pakistan. Afr. J. Biotech. 8, 6191-6200.
  • [38] Tomar, S. S., R. Singh and P. S. Singh, 2004. Response of phosphorus, sulphur and Rhizobium inoculation on growth, yield and quality of soybean. Prog. Agric. 4, 72-73.
  • [39] Van Reeuuwijk, L. P., 1992. Procedure for soil analysis, 4th ed, international soil reference and information center (ISRIC), Wageningen, Netherlands.
  • [40] Walkley, A. and C. A. Black, 1934. An examination of different methods for determining soil organic matter and the proposed modification by the chromic acid titration method. Soil Sci. 37, 29-38.
  • [41] Wall, L. G., A. Hellesten, K. Huss-Danell, 2000. Nitrogen, phosphorus and the ratio between them affect nodulation in Alnus incana and Wfolium prattense. Symbiosis 29, 91-105.
  • [42] Yohanes Uloro and Richer, 1999. Phosphorus efficiency of different variety of Phaseolus vulgaris and Sorgum bicolor (L). Moend on an Altisols in Eastern Ethiopia Highlands. Ethio. J. Nat. Res. 1, 187-200.
  • [43] Zuberer, D. A., 1998. Biological di-nitrogen fixation: Introduction and Non-symbiotic. In: Principles and Applications of Soil Microbiology. Prentice Hall, Inc. Simon & Schuster. A Viacom Company, Upper Saddle River, New Jersey, America.
Document Type
Publication order reference
YADDA identifier
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.