Acute Toxicity and Antidiabetic Effect of Polyherbal Formulation in Alloxan–Induced Diabetic Mice Model

• Authors: Le Ngoc Hung , Do Ngoc Thuy , Le Minh Ha , Phung Van Trung , Mai Van Nam , Nguyen Thi Huong
• Languages of publication: EN

Abstracts

EN

In Vietnam, many herbal plants with various medicinal activities can support different diseases such as anti-anemic and antimicrobial etc including diabetic treatments. Diabetes mellitus is a group of metabolic disorders that is characterized by elevated levels of glucose in the blood. When combining the herbs following a formula, the healing effect will be more effective due to synergetic actions. Our present study assesses acute toxicity and antidiabetic activity of a new polyherbal preparation, CHM-Tieu duong, in the form of capsules. It was developed based on the traditional use of six indigenous plants from the South-Western region of Vietnam, effective in fighting diabetes, namely Andrographis paniculata (leaves), Centella asiatica (leaves), Dioscorea opposite (tuberous rhizomes), Gymnema sylvestre (leaves), Gynostemma pentaphyllum (herbs), and Morus alba (leaves). Methods: The acute toxicity was evaluated following the Organization for Economic Cooperation and Development (OECD) guidelines No. 420 using five lots of female BALB/c albino mice. The antidiabetic activity was studied using an alloxan-induced diabetic mice model on thirty BALB/c albino mice of either sex. The mice were divided into five groups, namely normal mice control group with normal saline, diabetic mice fasted control group with normal saline, diabetic mice positive control group treated with the standard drug, metformin at a dose of 100 mg/kgP, and two diabetic mice groups treated with different doses of herbal combination at 500 and 1000 mg/kgP. Resutls: The acute toxicity analysis showed that CHM-Tieu duong was orally non-toxic at a single dose of 5000 mg/kgP as it did not cause any pre-clinical changes in experimental mice. The results of the antidiabetic study showed that CHM-Tieu duong exhibited the lowering glucose levels in the alloxan-induced diabetic mice model at the high dose of 1000 mg/kgP dose. Conclusion: The findings demonstrated that CHM-Tieu duong could serve as safe and effective antidiabetic polyherbal combination.

Keywords

EN

Acute toxicity; Alloxan-Induced Mice; Antidiabetic; Indigenous plants; Polyherbal combination; Southwestern region of Vietnam

Discipline

• PHARMACOLOGY: PHARMACOLOGY

• Publisher: Scientific Publishing House „DARWIN”
• Journal: World News of Natural Sciences
• Year: 2025
• Volume: 58
• Pages: 270-282

Contributors

author

Le Ngoc Hung

• Centre for High Technology Research and Development, Vietnam Academy of Sciene and Technology, Hoang Quoc Viet 18, 10000, Hanoi, Vietnam

author

Do Ngoc Thuy

• Centre for High Technology Research and Development, Vietnam Academy of Science and Technology, Hoang Quoc Viet 18, 10000, Hanoi, Vietnam

author

Le Minh Ha

• Institute of Natural Product Chemistry, Vietnam Academy of Science and Technology, Hoang Quoc Viet 18, 10000, Hanoi, Vietnam

author

Phung Van Trung

• Institute of Applied Materials Research, Vietnam Academy of Science and Technology, 01B TL29 Street, Thanh Loc, Dist. 12, 70000, HCM City, Vietnam

author

Mai Van Nam

• Hanoi University of Science and Technology, No 1 Dai Co Viet Street, Bach Khoa, Dist. Hai Ba Trung, 10000, Hanoi, Vietnam

author

Nguyen Thi Huong

• Centre for High Technology Research and Development, Vietnam Academy of Science and Technology, Hoang Quoc Viet 18, 10000, Hanoi, Vietnam

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• Document Type: article