Assessment of antiproliferative, cytotoxic and proapoptotic properties of selected flavonoids in relation to liver cancers – in vitro studies

• Authors: Tomasz Skalny , Anna Kleczka , Robert Kubina , Aleksandra Owczarzy , Magdalena Wyszyńska , Agata Kabała-Dzik

Title variants

PL

Ocena właściwości przeciwproliferacyjnych, cytotoksycznych i proapoptotycznych wybranych flawonoidów w stosunku do nowotworów wątroby w badaniach in vitro

• Languages of publication: EN PL

Abstracts

EN

INTRODUCTION: Flavonoids belong to phytotherapeutics with a wide spectrum of pharmacological activity. It has been proven that flavonoids possess properties that can inhibit the development of cancer by inducing cells into the programmed cell death process. The aim of the work was to demonstrate the apoptotic, antiproliferative and cytotoxic properties of selected flavonoids. MATERIAL AND METHODS: The test material was the human primary hepatocellular carcinoma SK Hep-1 cell line. The tested compounds were assessed for cytotoxicity with the MTT assay. The next step was to evaluate the level of protein expression from the Bcl-2 family using the human Bcl-2 ELISA test. RESULTS: The compound with the strongest cytotoxic properties confirmed by the MTT test is chrysin, the IC50 value of which was 316.67 μM/L. In the case of all the tested compounds, apoptotic processes were confirmed by the human Bcl-2 ELISA test. The highest level of Bcl-2 protein expression occurred after 48 hours after the administration of chrysin, hesperidin, naringin and kaempferol. CONCLUSIONS: On the basis of the obtained research results, it can be concluded that the studied flavonoids (chrysin, hesperidin, naringin, kaempferol) exhibit cytotoxic, proapoptotic and antiproliferative properties in relation to SK Hep-1 hepatoma cells.

PL

WSTĘP: Flawonoidy należą do fitoterapeutyków o szerokim spektrum działania farmakologicznego. Udowodniono, że flawonoidy posiadają właściwości mogące hamować rozwój choroby nowotworowej poprzez wywoływanie w komórkach procesu programowanej śmierci komórki. Celem pracy było wykazanie właściwości apoptotycznych, antyproliferacyjnych oraz cytotoksycznych wybranych flawonoidów. MATERIAŁ I METODY: Materiał do badań stanowiła ludzka linia komórkowa pierwotnego raka wątrobowokomórkowego. Badane związki poddano ocenie cytotoksyczności za pomocą testu MTT. Kolejnym etapem była ocena poziomu ekspresji białek z rodziny Bcl-2 testem Human Bcl-2. WYNIKI: Związkiem posiadającym najsilniejsze właściwości cytotoksyczne, potwierdzone testem MTT, jest chryzyna, której wartość IC50 wyniosła 316,67 μM/L. W przypadku wszystkich badanych związków stwierdzono zainicjowanie procesów apoptotycznych potwierdzone testem Human Bcl-2 ELISA. Najwyższy poziom ekspresji białek Bcl-2 miał miejsce po upływie 48 godzin od podania chryzyny, hesperydyny, narynginy i kemferolu. WNIOSKI: Na podstawie uzyskanych wyników badań można wnioskować, że badane flawonoidy (chryzyna, hesperydyna, naryngina, kemferol) wykazują właściwości cytotoksyczne, proapoptotyczne i antyproliferacyjne w stosunku do komórek raka wątrobowokomórkowego SK Hep-1.

Keywords

EN

Bcl-2; MTT; SK Hep-1; apoptosis; apoptoza; chrysin; chryzyna; hesperidin; hesperydyna; kaempferol; kemferol; naringin; naryngina

Discipline

• MEDICINE: MEDICINE

• Publisher: Śląski Uniwersytet Medyczny w Katowicach
• Journal: Annales Academiae Medicae Silesiensis
• Year: 2020
• Issue: 74
• Pages: 181-190

Contributors

author

Tomasz Skalny

• Department of Pathology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia in Katowice, Poland

author

Anna Kleczka

• Department of Pathology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia in Katowice, Poland

author

Robert Kubina

• Department of Pathology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia in Katowice, Poland

author

Aleksandra Owczarzy

• Department of Pathology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia in Katowice, Poland

author

Magdalena Wyszyńska

• Department of Pathology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia in Katowice, Poland

author

Agata Kabała-Dzik

• Department of Pathology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia in Katowice, Poland

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• Document Type: article