Antifungal activities of moulds associated with selected stored grains and legumes

• Authors: Ndidi Blessing Ohabughiro
• Languages of publication: EN

Abstracts

EN

An antifungal agent can either kill or inhibit the growth of fungi by interfering with the formation of fungal cell membrane, weakening it and hindering cell division. Antifungal agents of amphotericin B, ketoconazole, fluconazole and voriconazole of Thermo Fisher Scientific limited were used for this study. Cultural analysis of stored grains and legumes (rice, maize, wheat, groundnut and beans) from Imo State was done using streak plate method. Sabouraud dextrose agar was used for the culture while Mueller Hinton agar was used for Antifungal sensitivity test. Moulds were further identified using 18s rRNA gene sequencing method. The antifungal sensitivity profile of isolated and identified moulds was evaluated using the clinical laboratory and standard institute approved methods for testing of moulds, the disk diffusion and tetrazolium chloride test. The results showed that Aspergillus flavus, Aspergillus tamarii, Aspergillus niger, Aspergillus brunneoviolaceus and Penicillium chrysogenum were the moulds isolated and identified using both cultural and 18S rRNA sequence. The fungal isolates were susceptible to ketoconazole and voriconazole. Amphotericin B was both resistant and susceptible to different moulds. The fungal isolates were resistant to fluconazole. Inhibition effects were more with the antifungal disc than with tetrazolium salts. All the isolates were resistant to tetrazolium chloride and gave no zone of inhibition. Combination of antifungal agent and tetrazolium chloride showed sensitivity only to ketoconazole. Antifungal disc alone gave a better zone of inhibition than the combination of antifungal agents with tetrazolium salts. This study showed that ketoconazole has greater inhibitory potential than other antifungal agents. Ketoconazole remains the best drug of choice among the studied antifungal agents for fungal infections. Therefore antifungal drugs can be used against moulds of economic importance in the country.

Keywords

EN

Antifungal agents; Aspergillus brunneoviolaceus; Aspergillus flavus; Aspergillus niger; Aspergillus spp.; Aspergillus tamarii; Disk diffusion method; Penicillium chrysogenum; Stored grains and legumes

Discipline

• MICROBIOLOGY: MICROBIOLOGY

• Publisher: Scientific Publishing House „DARWIN”
• Journal: World News of Natural Sciences
• Year: 2023
• Volume: 50
• Pages: 52-59

Contributors

author

Ndidi Blessing Ohabughiro

• Department of Microbiology, Imo State University Owerri, P.M.B 2000, Owerri, Imo State, Nigeria

References

• [1] Kassam, A., Friedrich, T., Derpsch, R. and Kienzle, J. Overview of the Worldwide spread of Conservation Agriculture. The Journal of Field actions Science Report 8 (2016) 241-242
• [2] Andrew, A. M., and Beatrice, A.A Detection and Enumeration of moulds on some legumes and cereal grains from two local markets and two shopping malls in Accra Metropolis. African Journal of Microbiology Research, 18 (3), (2017) 1-9
• [3] Bossche, V.H., Engelen, M. and Rochette, F. Antifungal agents of use in animal health-chemical, biochemical and pharmacological aspects. J. Vet Pharmacol. Therap 26 (2003) 5-29
• [4] Fromtling, R.A. Overview of medically important antifungal azole derivatives. Clinical Microbiology Rev 1 (1988) 187-219
• [5] Clinical and Laboratory Standards Institute. Method for Antifungal Disk Diffusion Susceptibility Testing of Non dermatophyte Filamentous Fungi; Approved Guideline. CLSI document M51-A. Journal of Clinical Laboratory Analysis 13 (2) (2010) 31-32
• [6] Clinical and Laboratory Standards Institute. Performance Standards for Antifungal Disk Diffusion Susceptibility Testing of Filamentous Fungi; Informational Supplement CLSI document M51-S1. Journal of Clinical Laboratory Analysis 136 (4) (2011) 53-55
• [7] Albert, S., Vivian, S. and Gilbert, S.T. Antifungal properties of Tetrazolium compounds. Mycologia 48 (4) (1956) 473-483
• [8] Avasthi, S and Bhadauria, R. diversity, Pathogenicity and Toxicology of aspergillus niger. An important Spoilage Fungi. Res. J. Microbiology 6 (2011) 270-280
• [9] Beuchat, I.R. Enumeration of Fungi in Grain Flours and Meals as influence by settling time in diluent and by recovery Medium. Journal of Food Protection 55 (1992) 899-901
• [10] Tortorano, A. M., Richardson, M and Roilides, E. ESCMID and ECMM joint guidelines on diagnosis and management of hyalohyphomycosis. Fusarium spp, Scedosporium spp and others. Clinical microbiology Infection 20 (3) (2014) 27-46
• [11] Platt, A. R., Woodhall, R. W. and George, A.L. Improved DNA sequencing quality and efficiency using an optimized fast cycle sequencing protocol. Bio Techniques 43 (1) (2007) 58-62
• [12] Geogopapadakou, N.H., and Walsh, T.J. Antifungal agents: chemotherapeutic targets and immunologic strategies. Antimicrobial Agents Chemother 40 (1996) 279-291
• [13] Shaaban, H.N., Ahmed, A. T., Ahmed, A. A. and Favouk, A. Terrazolium /Formazan Test as an efficient method to determine fungal chitosan antimicrobial activity. Journal of Mycology 13 (2013) 7
• [14] Lopez, O. E., Aller., A.I. and Martin, C. Evaluation of disk diffusion method for determining susceptibility of filamentous fungi comparism with clinical laboratory standard institute broth microdilution method. Antimicrobial Agents Chemotherapy 50 (2006) 1109-1111
• [15] Agbulu, C.O., Iwodi, C. and Onekutu, A. In-Vitro susceptibility test of some antifungal drugs of selected Dermatophytes and yeast isolated from patients attending hospitals in Markurdi Environ. Microbiology Journal 5 (2015) 9-16
• [16] Kamal, U. Z., Abin, M., Richa, P. and Vijay, T. Antifungal susceptibility pattern of Candida albicans in human infections Biotechnology Pharmacology Laboratory and Human genetics Laboratory, Centre for Scientific Research and Development, People’s University, India. Open Biological Sciences Journal 4 (2018) 1-6
• [17] Kahan, M., Ikram, A., Zaman, A.G. and Farida, K.L. Pattern of antifungal susceptibility in pathogenic moulds by micro dilution method at a tertiary care hospital. Annals of Medical and Health Sciences Resource 8 (2018) 82-86
• [18] C. Manivannan, N. Santhi, Synthesis, characterization and antifungal activity of some fluorine containing 1,3,5-trisubstituted pyrazoline derivatives. World News of Natural Sciences 10 (2017) 86-94
• [19] Kindu Geta, Mekonen Wondimu, Emebat Ageze, Maritu Abebaw, Animut Workie and Bruktawit Getnet, Antifungal efficiency of three traditional medicinal plants against Trichophyton rubrum. World News of Natural Sciences 25 (2019) 15-21
• [20] S. Nisha, M. Saravanan, K. K. Ilavenil, Investigating the antibacterial and antifungal properties of few organic moieties: a comprehensive analysis. World News of Natural Sciences 50 (2023) 33-41
• [21] Lakshman R. Meena, Vinay S. Sharma, Pawan Swarnkar, Synthesis, biological investigations, QSAR and DFT analysis of sulfonamide chalcones as potential: antimicrobial, antifungal and antimalarial agents. World Scientific News 147 (2020) 179-196
• [22] P. I. Akwaji, E. J. Umana, E. I. Okon, Phytochemical and Antifungal Activity of Leaf Extracts of Corchorus olitorius and Gongronema latifolium on Fungi Associated with Post-Harvest Deterioration of Maize (Zea mays) Seeds in Oban Community, Nigeria. World Scientific News 53(3) (2016) 157-177

• Document Type: article