Preferences help
enabled [disable] Abstract
Number of results
2018 | 112 | 235-243
Article title

Morphological and cytometric indicators of the bream (Abramis brama Linnaeus, 1758) erythrocites from the Zaporizhian reservoir

Title variants
Languages of publication
The researches were carried out in the Zaporizhian reservoir (Ukraine) in the spring-summer period of 2015 - 2016. The samples were taken in two sections of the Zaporizhian reservoir which differ in ecological and hydrological regime: the Samara Bay (characterized by high content of heavy metals and high water mineralization) and the lower section of the reservoir (conventionally "clean zone"). The four-year-old specimens of both breeds of the Bream (Abramis brama Linnaeus, 1758) were the objects of the research. The fish were caught using gill nests during research fishing. The following indices of red blood cells were determined: large longitudinal and small transverse diameters of mature erythrocytes and their nuclei, erythrocyte area, erythrocyte core area. The volume of the cell, including the volume of the nucleus, was determined by the Houchin equation. It was established that in the the Bream from the Samara Bay an increase in the areas and volumes of fish red blood cells was observed compared to the same age species from the lower part of the reservoir. In erythrocytes of the researched fish, the following pathologies were observed: poikilocytosis, festonchity of the membrane, hyperchromia of the nucleus, anatomic location of the nucleus, amitose, invagination of the nucleus, nuclear shadows. The most pathologies were found in the Samara Bay, which sufferes from more intensive anthropogenic influences.
  • Oles Honchar Dnipro National University, Gagarina Avenue, 72, Dnipro, 49050, Ukraine
  • Oles Honchar Dnipro National University, Gagarina Avenue, 72, Dnipro, 49050, Ukraine
  • [1] Golovin, P.P.Problems of in fish stress in freshwater aquaculture: methods of diagnosis and correction, Collection of scientific papers. Diseases of fish. Moscow. Company Satellite. (2004) 54-61.
  • [2] Kuzina, Т.V. Analysis of hematological parameters of the pike perch from the Volga-Caspian canal. Natural Sciences 4 (29) (2009) 96-100.
  • [3] Adakole, J. A. Changes in some haematological parameters of the African catfish (Clarias gariepinus) exposed to a metal finishing company effluent. Indian Journal of Science and Technology, (5) (2012) 2510-2514.
  • [4] Sharamok, T. & Esipova. N. (2015). Influence of anthropogenic factors on fish hematological parameters. Scientific notes Ternopil National Pedagogical University, 3/4 (64) (2015) 722-726.
  • [5] Witeska, M. Erythrocytes in teleost fishe. Zoology and Ecology, 23(4) (2013) 1-4.
  • [6] Parfenova, I. & Soldatov, A. Functional morphology of circulating red blood cells in experimental hypoxia. Marine Ecological Journal, 2 (10) (2011) 59-67.
  • [7] Fedonenko, O.V., Yesipova, N.B., Sharamok, T.S., Ananyeva, Т.V. & Yakovenko, V.O. The modern problems of hydrobyology: Zaporizhian reservoir. Dnipropetrovs’k, Ukraine, LIRA. (2012) 279.
  • [8] Tase, K. Introduction to quantitative cytological morphology. Bucharest. The Acad. Rep. Romania. (1980) 291.
  • [9] Houchin, D.N., Munn, J.I. & Parnell, B.L. (1958). A method for the measurement of red cell dimensions and calculation of mean corpuscular volume and surface area. Blood, 13, 1185-1191.
  • [10] Andreeva, A.Y. Morphofunctional characteristics of erythrocytes Scorpaena Porcus L. in conditions of hypoxia (in vitro experiments). (PhD Thesis). Institute of evolutionary physiology and biochemistry Sechenova. St. Petersburg, Russia (2014).
  • [11] Jensen, F. B. Regulatory volume reduction in carp, red blood cells: mechanisms and oxygenation-dependence of volume-activated potassium and amino acid transport. Journal of Experimental Biology (1995) 155-165.
  • [12] Borgese, F., Garcia-Romeu, F. & Motais, R. Control of cell volume and ion transport by β-adrenergic catecholamines in erythrocytes of rainbow trout, Salmo gairdneri. Physiological Journal, 382 (1987) 123-144.
  • [13] Wood, C.M. & Simmons, H. The conversion of plasma HCO3- to CO2 by rainbow trout red cells in vitro: adrenergic inhibition and the effect of oxygenation status. Fish. Physiol. and Biochem. 12 (6) (1994) 445-454.
  • [14] Sharamok, T.S., Yesipova, N.B. & Kolesnik, N.L. Pathomorphological and cytometric indices of red blood cells of two-year-old crucian carp (Carassius auratus gibelio (Bloch, 1782). Fishery science of Ukraine, 3 (41) (2017) 98-108.
  • [15] Konkova, A.V. & Fedorova, N.N. Pathomorphological changes in red blood cells of young fish Abramia brama of the Volga-Caspian basin. Proceedings VNIRO, 162 (2016) 12-17.
  • [16] Liutinsky, S.I. Pathological physiology of animals. Moscow, Russia, Kolos. (2005) 496.
  • [17] Zhiteneva, L.D., Poltavtseva, T.G. & Rudnitskaya, O.A. Atlas of normal and pathologically altered blood cells of fish. Rostov-on-Don.Rost, Russia, Published. (1989) 112.
  • [18] Moiseenko, T.I. Hematological indicators of fish in the assessment of their toxicoses (on the example of the Coregonus lavaretus). Questions of ichthyology, 38 (3) (1989) 371-380.
  • [19] Kanieva, N.A. & Vorobiev, V.I. Influence of Caspian oil on fish (physiology, biochemistry and morphology). Astrakhan, Russia, Рublishing house of Astrakhan State Technical Universit. (1960) 180.
  • [20] Lukin, A.A., Sharova, Y.N. & Bilicheva, L.A. Assessment of the state of the fish body in conditions of contaminated water systems with oil products and waste products of pulp and paper production. Fishery, (6) (2010) 47-52.
  • [21] Izergina E.E., Izergin I.L. and Izergin L.I. Atlas of blood cells of salmonids on the mainland coast of the northern part of the Sea of Okhotsk. Magadаn, Russia, Cordis. (2014) 127.
  • [22] Korolyova I.M. Hematologic indices of whitefishes in the reservoirs of the Kola North under anthropogenic load. In V.R. Mikryakova, E.A. Kriksunova & D.V. Mikryakova (Eds.). Problems of pathology, immunology and protection of fish and other aquatic organisms: Extended. mater. IV International Conference. Borok .Yaroslavl, Russia, Filigree. (2015) 588
Document Type
Publication order reference
YADDA identifier
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.