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2017 | 11 | 91-97
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Total Phenolic, Flavonoid Contents and Antioxidant Activities of Honey and Propolis Collected from the Region of Laghouat (South of Algeria)

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The aim of this study was to determine total phenolic, flavonoid contents and to evaluate antioxidant activities of two honeys and one propolis samples, collected from the region of Laghouat (South of Algeria). Total phenolic contents were determined by using Folin-Ciocalteu reagent as gallic acid equivalent and flavonoids using AlCl3 method as Rutin equivalent. Antioxidant activities of the honeys and propolis were examined by two different methods, namely scavenging of free radical 2,2-diphenyl-1-picrylhydrazyl and Reducing power. The antioxidant activities were compared with standard antioxidants such as Ascorbic acid, BHT and Trolox. The highest level of phenolic was 2385 mg Gallic acid per 100g sample, the highest level of flavonoid was 379 mg Rutin per 100g sample and the highest protein content was 1177 mg per 100g sample, DPPH (0.026 mg/ml) and TEAC (0.0015) were detected especially the propolis sample, indicating good antioxidant properties. A strong positive correlation was found between phenolics, flavonoids, DPPH and TEAC indicating that in addition to total phenolic, flavonoid and protein concentrations are good indicators of the antioxidant potential of propolis.
References
  • [1] Kumazawa, H.T. and Nakayama, T. 2004. Antioxidant activity of propolis of various geographic origins. Food Chem. 84, 329-339
  • [2] Aljadi, A.M. and Kamaruddin, M.Y. 2004. Evaluation of the phenolic contents and antioxidant capacities of two Malaysian floral honeys. Food Chem. 85(4), 513-518
  • [3] Ahn, M.R., Kumazawa, S., Usui,Y., Nakamura, J., Matuska, M., Zhu, F., Nakayama, T., 2007. Antioxidant activity and constituents of propolis collected in various areas of China. Food Chemistry 101, 1383-1392
  • [4] Singleton,V.L., Rossi, J.R., 1965. Colorimetry of total phenols with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture 16, 144-158
  • [5] Lowry H., Nira J., Rosebrough A., Farr L., Rose J.R., Protein measurement with the Folin phenol reagent. J Biol Chem 1951, 193, 265-275
  • [6] Oyaizu, M. (1986). Studies on products of browning reactions: Antioxidative activities of products of browning reaction prepared from glucosamine. Japanese Journal of Nutrition, 44, 307-315
  • [7] Gómez-Caravaca, A.M., Gómez-Romero, M., Arráez-Román,D., Segura-Carretero,A., & Fernández-Gutiérrez, A. (2006). Advances in the analysis of phenolic compounds in products derived from bees. Journal of Pharmaceutical and Biomedical Analysis, 41(4), 1220-1234
  • [8] Brand-Williams W., Cuvelier E., and Berset C. (1995), Use of free radical method to evaluate antioxidant activity. Food Sci. Technol. 28, 25-30
  • [9] Khalil, M. I.; Alam, N.; Moniruzzaman, M.; Sulaiman, S. A.; Gan, S. H. Phenolic acid composition and antioxidant properties of Malaysian Honeys. Journal of Food Science 2011, 76, 921
  • [10] Sataruba, R.; Subha, G. Physical, chemical and antioxidant properties of honey: A review. Asian Journal Chemical Pharmaceutical Research 2014, 2, 96
  • [11] Kuçuk, M.; Kolayli, S.; Karaoglu, S. A.; Ulusoy, E.; Baltaci, C.; Candan, F. Biological activities of three different Turkish honeys. Food Chemistry 2007, 100, 526
  • [12] Baroni, M. V., Nores, M. L., Díaz, M.D. P., Chiabrando, G. A., Fassano, J. P., Costa, C., et al. Determination of volatile organic compound patterns characteristics of five unifloral honeys by solid-phase microextraction - Gas chromatography - mass spectrometry coupled to chemometrics. Journal of Agricultural and Food Chemistry 2006, 54, 7235
  • [13] Boufadi Y.M , Soubhye J., Riazi A. et al. Characterization and Antioxidant Properties of Six Algerian Propolis Extracts: Ethyl Acetate Extracts Inhibit Myeloperoxidase Activity. Int. J. Mol. Sci. 2014, 15, 2327-2345
  • [14] Nair S and Raho G. B., Evaluation of antioxidant properties of propolis collected in north-west of algeria. international journal of biology. Pharmacy and allied Sciences 2017, 6(3), 494-503
  • [15] Miguel, M. G., Nunes, S., Dandlen, S. A., Cavaco, A. M. and Antunes, M. D. Phenols, flavonoids and antioxidant activity of aqueous and methanolic extracts of propolis (Apis mellifera L.) from Algarve, South Portugal. Food Science and Technology 2014, 34 (1), 16-23.
  • [16] Belfar M. L , Lanez T., Rebiai A., Ghiaba Z., Evaluation of Antioxidant Capacity of Propolis Collected in Various Areas of Algeria Using Electrochemical TechniquesInt. J. Electrochem. Sci. 10 (2015) 9641-9651
  • [17] Borrelli F, Maffia P, Pinto L, Ianaro A, Russo A, Capasso F, Ialenti A. 2002. Phytochemical compounds involved in the anti-inflammatory effect of propolis extract. Fitoterapia 73(1), 53-63
  • [18] Al-Mamary M, Al-Meeri A, Al-Habori M. 2002. Antioxidant activities and total phenolics of different types of honey. Nutrition Research 22, 1041-1047
  • [19] Gheldof N, Wang XH, Engeseth NJ. 2002. Identification and quantification of antioxidant components of honeys from various floral sources. Journal of Agricol and Food Chemetry 50, 5870-5877
  • [20] Johnston JE, Sepe HA,Miano CL, Brannan RG, Alderton AL. 2005. Honey inhibits lipid oxidation in ready-to-eat ground beef patties. Meat Science 70, 627-631
  • [21] Kücük M, Kolayli S, Karaoglu S, Ulusoy E, Baltaci C, Candan F. 2007. Biological activities and chemical composition of three honeys of different types from Anatolia. Food Chemistry 100, 526-534
  • [22] Ouchemoukh S, Louaileche H, Schweizer P: Physicochemical characteristics and pollen spectrum of some Algerian honeys. Food Control 2007, 18, 52-58
  • [23] Meda A, Lamien CE, Romito M, Millogo J, Nacoulma OG: Determination of the total phenolic, flavonoid and proline contents in Burkina Fasan honey, as well as their radical scavenging activiity. Food Chem 2005, 91, 571-577
  • [24] Debab M. Toumi-Benali F, Dif M.M. 2016. Antioxidant Activity of Propolis of West Algeria. Phytothérapie. DOI10.1007/s10298-016-1085-9
  • [25] Popova, M. P., Bankova, V. S., Bogdanov, S., Tsvetkova, I., Naydenski, C., Marcazzan, G. L., & Sabatini, A. G. (2007). Chemical characteristics of poplar type propolis of diferent geographic origin. Apidologie, 38, 306-311
  • [26] Haung D, Ou B, Prior RL (2005). The chemistry behind antioxidant capacity assays. J. Agric. Chem. 53, 1841-1856
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article
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bwmeta1.element.psjd-a66c526e-1a87-4b1d-88f6-9b7c4463b299
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