Ameliorative Effect of Ethanolic Extract of Laportea aestuans (L.) Chew on Gastric Oxidative Damage in Aspirin - Induced Gastric Ulcer in Male Rats

• Authors: Akinwande Onadeko , Olusegun Akinola
• Languages of publication: EN

Abstracts

EN

Non-steroidal anti-inflammatory drugs (NSAIDs) are connected with the generation of free radicals and one of its adverse effects is gastric ulceration. Ulceration was induced orally using aspirin. Twenty-four male Wistar rats were used for this study (120-150g). Rats were divided into 6 groups with each group containing 4 rats. Rats were pre-treated orally with cimetidine, a reference drug. Group 1 rats orally received 1% gum acacia solution as the control group, Group 2 rats orally administered 25 mg/kg aspirin and served as the ulcerated, untreated group, rats in groups 3 and 4 were pre-treated orally with 200 mg/kg and 400 mg/kg respectively for 3 days while rats in groups 5 and 6 were pre-treated orally with 50 mg/kg cimetidine and 50 mg/kg catechin respectively for 3 days. The result of this study shows that the ulcerated, untreated rats showed increased concentrations of gastric volume with a concomitant decrease in protein concentration compared to the control group, cimetidine and catechin reversed these observations. Activities of glutathione, catalase were decreased in the ulcerated, untreated group but pre-treatment with different doses, cimetidine and catechin reversed these observations. In conclusion, the ethanoic extract of L. aestuans can be said to be used as an anti-ulcerogenic agent against aspirin-induced gastric ulcer which is due to the presence of phytochemicals in the plant.

Keywords

EN

Aspirin; Cimetedine; Gastric Ulcer; Laportea aestuans; Oxidative Stress; Phytochemicals

Discipline

• BIOCHEMISTRY: BIOCHEMISTRY

• Publisher: Scientific Publishing House „DARWIN”
• Journal: World News of Natural Sciences
• Year: 2021
• Volume: 39
• Pages: 86-94

Contributors

author

Akinwande Onadeko

• Department of Biochemistry, Bowen University, Iwo, Osun State, Nigeria

author

Olusegun Akinola

• Department of Biochemistry, Bowen University, Iwo, Osun State, Nigeria

References

• [1] Oloyede GK, Ayanbadejo OE. Phytochemical, toxicity, antimicrobial and antioxidant screening of extracts obtained from Laportea aestuans (Gaud). Journal of Medical Science. 2014. 14, 51–59
• [2] Panda V, Khambat P. Evaluation of antacid activity of Garcinia indica fruit rind by a modified artificial stomach model. Bulletin of Environment, Pharmacology and Life Sciences. 2013. 2, 38–42
• [3] Harmon RC, Peura DA. Evaluation and management of dyspepsia. Therapeutics Advances in Gastroenterology. 2010. 3, 87–98
• [4] Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry, 1951. 183: 265-275
• [5] Sharma S, Dwivedi J, Paliwal S. Evaluation of antacid and carminative properties of Cucumis sativus under simulated conditions. Der Pharmacia Letter. 2012. 4, 234–239
• [6] Beutler EO, Duron, Kelly BM. Improved method for the determination of blood glutathione. Journal of Laboratory and Clininical Medicine, 1973. 61: 882-888
• [7] Sinha KA. Calorimetric assay of catalase. Analytical Biochemistry, 1971. 47: 389-394.
• [8] Saravanan S, Dhasarathan P, Indira V, Venkatraman R. Gastro Protective and Antioxidant Activity of Solanum nigrum linn. Against Aspirin and Cold Restraint Stress induced Ulcerated Rats. Research Journal of Immunology, 2011. 4: 1-11
• [9] Petkau A, Chelack W, Pleskach S, Meeker B, Brady C. Radioprotection of Mice by Superoxide Dismutase , Biochemical and Biophysical Research Communications, 1975. 65, 88
• [10] Deoda RS, Dinesh Kumar, Bhujbal.SS. Gastroprotective Effect of Rubia cordifolia Linn. on Aspirin Plus Pylorus-Ligated Ulcer. Evidence-Based Complementary and Alternative Medicine. 2011. 2011.
• [11] Repetto MG, Lleusy SF. Antioxidant properties of natural compounds used in popular medicine for gastric ulcers. Brazilian Journal of Medical Biological Research. 2002. 35: 523-534
• [12] Takeuchi K, Amagase K. Roles of Cyclooxygenase, Prostaglandin E2 and EP receptors in mucosal protection and ulcer healing in the gastrointestinal tract, Current Biophysical Pharmacology, 2018. 24 (18) 2002-2011
• [13] Wang Z, Hasegawa J, Wang X, Matsuda A, Tokuda T, Miura N, Watanabe T. Protective effects of ginger against aspirin-induced gastric ulcers in rats, Yonago Acta Medica, 2011. 54, 11–19
• [14] Patel D, Defer JN, Stewart JD. Pepsin as a marker of gastric ulcer. American Journal of Physiology. 2001. 3:18-21
• [15] Alada ARA, Ajayi FF, Alaka OO, Akande OO. Gastric Acid Secretion and Experimental Ulceration in rats fed Soybean Diet Preparations. African Journal of Biomedical Research, 2005. 8; 203-205
• [16] Das SK, Roy C. The protective role of Aegle marmelos on aspirin-induced gastro-duodenal ulceration in albino rat model: a possible involvement of antioxidants. Saudi Journal of Gastroenterology, 2012. 18(3): 188-94
• [17] Raza H, John A. Implications of altered glutathione metabolism in aspirin-induced oxidative stress and mitochondrial dysfunction in HepG2 cells. PLoS One. 2012.7(4).
• [18] Hawaii Pacific Weed Risk Assessment. Clifford P.Chimera C. Available at: http://www.hear.org/wra/ [accessed 3rd June, 2021]
• [19] Fornai M, Blandizzi C, Colucci R, Antonioli L, Bernardini N, Seqnani C.Role of cyclooxygenases 1 and 2 in the modulation of neuromuscular functions in the distal colon of humans and mice. Gut, 2005. 54, 608-616
• [20] Halabi MF, Shakir RM, Bardi DA, Al-Wajeeh NS, Ablat A, Hassandarvish P, Hajrezaie, Norazit MA, Abdulla MA. Gastroprotective activity of ethyl-4-[(3,5-di-tert-butyl-2-hydroxybenzylidene) amino] benzoate against ethanol-induced gastric mucosal ulcer in rats, PLoS One 9, 2014. 95908
• [21] Handa O, Naito Y, Fukui A, Omatsu T, Yoshikawa T. The impact of non-steroidalanti-inflammatory drugs on the small intestinal epithelium. Journal of Clinical Biochemistry and Nutrition 54, 2014. 2-6
• [22] Bandyopadhyay U, Biswas K, Chatterjee R, Bandyopadhyay D, Chattopadhyay I, Ganguly CK. Gastroprotective effect of Neem (Azadirachta indica) bark extract: Possible involvement of H (+) - K (+) ATPase inhibition and scavenging of hydroxyl radical. Life Science, 2002. 71: 2845-65
• [23] Cho CH, Ogle CW. Cholinergic- mediated gastric mast cell degradation with subsequent histamine H1 and H2 receptor activation in stress ulceration in rats. European Journal of Pharmacology, 1979. 55: 23-33
• [24] Sarkar S, Buha D. Effect of ripe fruit pulp extract of Cucurbita pepo Linn. in aspirin induced gastric and duodenal ulcer in rats. Indian Journal of Experimental Biology, 2008, 46: 639-645
• [25] Victor Uchechukwu Okechukwu, Sunday Onyekwere Eze, Daniel Omeodisemi Omokpariola, Janefrancess Chinemerem Okereke, Evaluation of phytochemical constituents of Methanol extract of Moringa oleifera Lam. whole leaf by Gas Chromatography-Mass Spectrometry and Fourier transform infrared spectroscopy analysis. World News of Natural Sciences 37 (2021) 18-30
• [26] C. P. Ravindran, M. Manokari, Mahipal S. Shekhawat, Biogenic production of zinc oxide nanoparticles from aqueous extracts of Duranta erecta L. World Scientific News 28 (2016) 18-28
• [27] O. Oyewole, E. Aondoakaa, T. S. Abayomi, S. J. Ogundipe, T. A. Oshin, Characterization and optimization study of Ficus exasperata extract as corrosion inhibitor for mild steel in seawater. World Scientific News 151 (2021) 78-84
• [28] L. N. Emembolu, O. D. Onukwuli, V. N. Okafor, Characterization and optimization study of Epiphyllum oxypetalum extract as corrosion inhibitor for mild steel in 3 M H2SO4 solutions. World Scientific News 145 (2020) 256-273

• Document Type: article