Entomotoxicity properties of eco-friendly methanol extract fractions from Phyla nodiflora (L.) Greene leaf exhibits mosquito larvicidal, pupicidal and antimicrobial activity

• Authors: H. Irrusappan , J. Gokulakrishnan , K. Elumalai , S. Senthilmurugan , P. Vijayan , M. Baranitharan
• Languages of publication: EN

Abstracts

EN

Mosquitocidal activity of Phyla nodiflora methanol leaf extract fractions (Pn-MLEFr) was controlled and using main components on malarial vector, Anopheles stephensi (An. stephensi). Pn-MLE were characterized utilizing tools containing TLC, CC, FTIR and GCMS, and it against 3rd instars larvae of An. stephensi, followed by pupicidal activity was determined to concentration of 1, 3, 5 ppm. In phytochemical, characterized by GCMS analysis was carried out to be eligible for the constituents of the MLE. Pn-MLE Fr-6 showed the highest LC50 and LC90 values of 25.80 and 68.45 ppm, respectively. Fr-6 was found to be most effective for this activity provided pupa stage at death and stage of non-mortality values were 22.85 (NDP), 76.1 (TDP), 1.2 (DP), 0.7 (DA), 78% (TM%) and 6.6 (NAE), 22 (AE%) at 1 ppm against An. stephensi. Moreover, 3 and 5 ppm were provided TM% and AE% values of 94.43% and 5.57%; 100% and no adults emerged. In GC-MS analyzes, a total of 21 compounds were identified in the Pn-MLE, the main component was Ergosta-5,22-dien-3-ol, acetate, (3á,22E)-. Further, the AgNPs synthesized using Fm-ALE showed enhanced anti-bacterial activity. The reports revealed the Ergosta-5,22-dien-3-ol, acetate, (3á,22E)- was the most one of the important a compound provides malarial vector control from Pn-MLEFr.

Keywords

EN

Anopheles stephensi; GC-MS; Phyla nodiflora; mosquito larvae

Discipline

• ZOOLOGY: ZOOLOGY

• Publisher: Scientific Publishing House „DARWIN”
• Journal: World News of Natural Sciences
• Year: 2022
• Volume: 42
• Pages: 107-122

Contributors

author

H. Irrusappan

• Technical Support Unit, NCVBDC, New Delhi-110054, India

author

J. Gokulakrishnan

• Department of Zoology, Poompuhar College (Autonomous), Melaiyur-609107, Tamil Nadu, India

author

K. Elumalai

• Department of Advanced Zoology & Biotechnology, Government Arts College for Men (Autonomous), Chennai-600035, Tamil Nadu, India

author

S. Senthilmurugan

• Department of Zoology, Annamalai University, Annamalainagar-608 002, Tamil Nadu, India

author

P. Vijayan

• Department of Zoology, Annamalai University, Annamalainagar-608 002, Tamil Nadu, India

author

M. Baranitharan

• Department of Zoology, Poompuhar College (Autonomous), Melaiyur-609107, Tamil Nadu, India

References

• [1] World Health Organization, World Malaria Report 2019, World Health Organization, Geneva, Switzerland (2019).
• [2] Baranitharan M, Krishnappa K, Pandiyan J, Gokulakrishnan J, Kovendan K, Tamizhazhagan V. Citrus limetta (Risso) - borne compound as novel mosquitocides: Effectiveness against medical pest and acute toxicity on non-target fauna. South African Journal of Botany 128 (2020) 218-224
• [3] Subash B, Vijayan P, Baranitharan M. Biosynthesis of silver nanoparticles using Hygrophila auriculata: A novel route of malarial fever vector mosquito control. International Journal of Scientific & Technology Research 8 (2019) 4010-4018.
• [4] Surendran SN, Sivabalakrishnan K, Sivasingham A, Jayadas TTP, Karvannan K, Santhirasegaram S, et al. Anthropogenic factors driving recent range expansion of the malaria vector Anopheles stephensi. Front Public Health 7 (2019). https://doi.org/10.3389/fpubh.2019.00053
• [5] World Health Organization. Vector alert: Anopheles stephensi invasion and spread [cited 2020 Sep 3]. https://www.who.
• [6] Duke JA and Ayensu ES. Medicinal Plants of China Reference Publications, Inc. 1985 ISBN 0-917256-20p-4.
• [7] PRSA (Plant Resources of Southeast Asia). http://proseanet.org/.
• [8] Abbott WS. A method of computing the effectiveness of an insecticide. Journal of Economic Entomology 18 (1925) 265-267
• [9] Vivek M, Kumar S, Steffi P, Sudha SS. Biogenic silver nanoparticles by Gelidiella acerosa extract and their antifungal effects. Avicenna Journal of Medical Biotechnology 3(3) (2011) 143–148
• [10] Kumaravel S, Praveen Kumar P, Vasuki P. GC-MS study on microbial degradation of Lindane. International Journal of Applied Chemistry 6 (2010) 363-366
• [11] Finny DJ. A statistical treatment of the sigmoid response curve. In: Probit analysis. Cambridge University Press, London 633 (1971)
• [12] Baranitharan M, Dhanasekaran S, Murugan K, Kovendan K, Gokulakrishnan J. Chemical composition and laboratory investigation of Melissa officinalis essential oil against human malarial vector mosquito, Anopheles stephensi L. (Diptera: Culicidae). Journal of Coastal Life Medicine 4 (2016) 969-973.
• [13] Benelli G. Plant-mediated synthesis of nanoparticles: anewer and safer tool against mosquito-borne diseases? Asian Pacific Journal of Tropical Biomedicine 6 (2016) 353-354
• [14] Elijah Eze Ajaegbu, Simon Pierre Yinyang Danga, Ikemefuna Uzochukwu Chijoke, Festus Basden Chiedu Okoye. Mosquito adulticidal activity of the leaf extracts of Spondias mombin L. against Aedes aegypti L. and isolation of active principles. Journal of Vector Born Diseases 53 (2016) 17-22
• [15] Swati Supare, Mansi Patil. Estimation of phytochemical components from Cassia tora and to study its larvicidal activity. International Journal of Pharmaceutical Science Invention 4 (2015) 11-16
• [16] Baranitharan M, Dhanasekaran S, Gokulakrishnan J, Krishanappa K, Deepa J. Mosquito larvicidal properties of Sesamum indicum L. against Aedes aegypti (Linn.), Anopheles stephensi (Liston), Culex quinquefasciatus (Say) (Diptera: Culicidae). Life Science Archives 3 (2015) 130-136
• [17] Panneerselvam C, Murugan K, Kovendan K, Mahesh Kumar P and Subramaniam J, Mosquito larvicidal and pupicidal activity of Euphorbia hirta Linn. (Family: Euphorbiaceae) and Bacillus sphaericus against Anopheles stephensi Liston. (Diptera: Culicidae). Asian Pacific Journal of Tropical Medicine 6(2) (2013) 102-109. DOI: 10.1016/S1995-7645(13)60003-6
• [18] Govindarajan M, Jebanesan A, Pushpanathan T. Larvicidal and ovicidal activity of Cassia fistula Linn. leaf extract against filarial and malarial vector mosquitoes. Parasitology Research 102 (2008) 289-292
• [19] Baranitharan M, Dhanasekaran S. Mosquito larvicidal properties of Commiphora caudate (Wight & Arn.) (Bursaceae) against Aedes aegypti (Linn.), Anopheles stephensi (Liston), Culex quinquefasciatus (Say). International Journal of Current Microbiology and Applied Sciences 3 (2014) 262-268
• [20] Baranitharan M, Dhanasekaran S, Mahesh Babu S, Sridhar N. Larvicidal activity of Croton Sparciflorus Morong (Euphorbiaceae) leaf extracts against three vector mosquitoes. Science Park Research Journal 1 (2014) 1-7. DOI:10.9780/23218045/1202013/49
• [21] David JP, Rey D, Pautou MP, Meyran JC. Differential toxicity of leaf litter to dipteran larvae of mosquito developmental sites. Journal of Invertebrate Pathology 75 (2000) 9-18
• [22] Patil SV, Patil C.D, Salunkhe RB, Salunke BK. Larvicidal activities of six plants extracts against two mosquito species, Aedes aegypti and Anopheles stephensi. Tropical Biomedicine 27 (2010) 360-365
• [23] Mohankumar TK, Shivanna KS, Achuttan VV. Screening of Methanolic Plant Extracts against Larvae of Aedes aegypti and Anopheles stephensi in Mysore. Journal of Arthropod-Borne Diseases 10 (2016) 305-316
• [24] Baranitharan M, Tamizhazhagan V, Kovendan K, Senthilmurugan S. Punica Granatum-based green ethanolic extract as highly effective and eco-friendly larvicide, repellent against medically important mosquito vectors. Entomology and Applied Science Letters 6 (2019) 33-41
• [25] Ke-Xin Yu, Ching-Lee Wong, Rohani Ahmad and Ibrahim Jantan, Mosquitocidal and oviposition repellent activities of the extracts of seaweed Bryopsis pennata on Aedes aegypti and Aedes albopictus. Molecules 20 (2015) 14082-14102
• [26] Baranitharan M, Dhanasekaran S, Murugan K, Kovendan K, Gokulakrishnan J and Benelli G. Coleus aromaticus leaf extract fractions: A source ofnovel ovicides, larvicides and repellents against Anopheles, Aedes and Culex mosquito vectors?. Process Safety and Environmental Protection 106 (2017) 23-33.
• [27] Jebanesan, A., Baranitharan, M., Kovendan, K. et al. Impact of Punica granatum-based green larvicide on the predation rate of Polypedates cruciger for the control of mosquito vectors, Anopheles stephensi and Culex quinquefasciatus (Diptera: Culicidae). Int J Trop Insect Sci 41, 1075–1085 (2021). https://doi.org/10.1007/s42690-020-00293-7
• [28] Nurhayat Tabanca, Zulfiqar Ali, Ulrich R. Bernier, Nancy Epsky, Ayse Nalbantsoy, Ikhlas A. Khan, Abbas Ali (2018). Bioassay-guided isolation and identification of Aedes aegypti larvicidal and biting deterrent compounds from Veratrum lobelianum. Open Chemistry 16 (2018) 324-332
• [29] Perumalsamy H, Jang MJ, Kim JR, Kadarkarai M, Ahn YJ. Lar¬vicidal activity and possible mode of action of four flavonoids and two fatty acids identified in Millettia pinnata seed toward three mosquito species. Parasites and Vectors 8 (2015) 237, https//doi.org/10.1186/s13071-015-0848-8
• [30] M. Muni Raj, G. Srikanth, M. Rajikkannu, R. Nandakumar, Evaluation of botanicals against: Mosquito Larvae to the Extracts of Fungus Beauveria Species. World Scientific News 88(2) (2017) 199-210

• Document Type: article