PL EN


Preferences help
enabled [disable] Abstract
Number of results
2017 | 66 | 75-85
Article title

In Vitro Micropropagation of Drosera rotundifolia

Content
Title variants
Languages of publication
EN
Abstracts
EN
The aim of the study was to determine an optimal composition of growth media for Drosera rotundifolia L. cultured in vitro. Adventitious shoots of Drosera rotundifolia originating from an established in vitro culture were placed on complete Murashige and Skoog (MS) (1962) medium, MS medium with nutrient content reduced to ¾ MS, ½ MS, ¼ MS, 1/6 MS and MS medium with selected macronutrient (KNO3, KNO3 + NH4NO3, KH2PO4 or CaCl2) content reduced by ½. The medium containing ¼ of standard MS nutrients was found the most effective, as plants in this variant had the greatest weight and developed the greatest number of adventitious shoots (37.1). It was also conducive to root growth and development. Flowering was only observed on complete MS, ¾ MS and ½ MS media and MS with reduced content of KNO3 or KNO3 + NH4NO3. Reducing mineral content to ¼ MS or more resulted in red coloration of glandular tentacles.
Year
Volume
66
Pages
75-85
Physical description
Contributors
author
  • Department of Genetics, Plant Breeding and Biotechnology, West Pomeranian University of Technology in Szczecin, 17 Słowackiego Str., 71-434 Szczecin, Poland, Paula.Jadczak@zut.edu.pl
author
  • Department of Genetics, Plant Breeding and Biotechnology, West Pomeranian University of Technology in Szczecin, 17 Słowackiego Str., 71-434 Szczecin, Poland
  • Department of Genetics, Plant Breeding and Biotechnology, West Pomeranian University of Technology in Szczecin, 17 Słowackiego Str., 71-434 Szczecin, Poland
References
  • [1] R. Banasiuk, A. Kawiak, A. Krölicka. In vitro cultures of carnivorous plants from the Drosera and Dionaea genus for the production of biologically active secondary metabolites. Biotechnologia 93(2) (2012) 87-96.
  • [2] M. Bobák, A. Blehová, J. Krißtin, M. Oveçka, J. Samaj. Direct plant regeneration from leaf explants of Drosera rotundifolia cultured in vitr. Plant Cell Tiss. Org Cult. 43 (1995) 39-49.
  • [3] Burbidge. Secondary plant metabolites from tissue culture. w: In vitro cultivation of plant cells. Ed. Hunter C. F. Butterworth Heinemann, London, (2004) 131-151.
  • [4] G. Foot, S.P. Rice, J. Millett. Red trap colour of the carnivorous plant does not serve a prey attraction or camouflage function. Biology Letters 10(4) (2014) 20131024
  • [5] T. Grevenstuk, N. Coelho, S. Gonçalves & A. Romano. In vitro propagation of Drosera intermedia in a single step. Biologia plantarum, 54(2) (2010) 391-394.
  • [6] S. Ichiishi, T. Nagamitsu, Y. Kondo, T. Iwashina, K. Kondo, N. Tagashira. Effects of Macro-components and Sucrose in the Medium on in vitro Red-color Pigmentation in Dionea muscipula Ellis and Drosera spathulata Labill. W: Plant Biotechnology, Hiroshima (1999) 235-238.
  • [7] P. Karlsson, J. Pate. Contrasting effects of supplementary feeding of insects or mineral nutrients on the growth and nitrogen and phosphorus economy of pygmy species of Drosera. Oecologia (1994) 14.
  • [8] Kawiak, A. Królicka, E. Lojkowska. Direct regeneration of Drosera from leaf explants and shoot tips. Plant Cell, Tissue and Organ Culture 75(2) (2003) 175-178.
  • [9] A Kawiak, J. Zawacka-Pankau, E. Lojkowska. Plumbagin induces apoptosis in Her2-overexpressing breast cancer cells through the mitochondrial-mediated pathway. Journal of Natural Products 75(4) (2012) 747-751.
  • [10] Kawiak, J. Zawacka-Pankau, A. Wasilewska, J. Bigda, E. Lojkowska. Induction of apoptosis in HL-60 cells through the ROS-mediated mitochondrial pathway by ramentaceone from Drosera aliciae. Journal of Natural Products 75(1) (2012) 9-14.
  • [11] L. Krenn, T. Kartnig, Phytotherapie, Frankfurt (1987) 197-202.
  • [12] Krolicka, A. Szpitter, M. Maciag, E. Biskup, E. Gilgenast, G. Romanik, E. Lojkowska. Antibacterial and antioxidant activity of the secondary metabolites from in vitro cultures of the Alice sundew (Drosera aliciae). Biotechnology and applied biochemistry 53(3) (2009) 175-184.
  • [13] K. Kukulczanka & B. Czastka. Vegetative propagation of were successfully transferred to peat. selected species of Droseraceae taxa and in vitro gene bank establishment. Prac. Ogr. Bot. PAN 1 (1991) 55-61.
  • [14] F. E. Lloyd. The Carnivorous plants, Chronica Botanica Company of Waltham, Mass., U.S.A (1942).
  • [15] T. Murashige, F. Skoog. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum (1962) 473-497
  • [16] J. Rejthar, I. Viehmannova, P. H. Cepkova, E. Fernández & L. Milella. In vitro propagation of Drosera intermedia as influenced by cytokinins, pH, sucrose, and nutrient concentration. Emirates Journal of Food and Agriculture 26(6) (2014) 558.
  • [17] U. Stichmann-Marny, E. Kretzschmar. Przewodnik. Rośliny i Zwierzęta, Multico, Warszawa, (1997) 284.
  • [18] S. G. Vásquez-Collantes, C. Rojas-Idrogo, G. E. Delgado-Paredes. In vitro flowering and plantlets elongations in „Sundew” Drosera capillaries. International Journal of Plant, Animals and Environmental Sciences (2014) 513-515.
  • [19] C. Wawrosch, An improved 2-step liquid culture system for efficient in vitro shoot proliferation of Sundew (Drosera rotundifolia L.) Sci. Pharm. Vienna (2002) 251-262.
Document Type
article
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.psjd-95c6ea4e-79a6-438e-bd37-1eb9a1975618
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.