Isolation of Flavonoids, Antioxidant and Antimicrobial Activities of Costus afer Ker Gawl. Stem Extracts

• Authors: Israel Azogor Ekoro , Mary Olire Edema , Christiana Ene Ogwuche
• Languages of publication: EN

Abstracts

EN

ABSTRACT Resistance to antimicrobial agents has become an increasingly important and pressing global problem. Hence, the need for substantial investment and research in the field of anti-infectives are now desperately needed if a public health crisis is to be averted. This study aims to determine the antioxidant and antimicrobial activity of Costus afer stem and isolate the flavonoids in the extracts. The method used for isolation was a combination of thin layer chromatography (TLC) and column chromatography (CC). Phytochemical screening tests were used for identification of the eluate fractions of CC to ascertain the flavonoid-rich fraction. The flavonoid content in dry stem extracts (DSE); 153 µg/g was lesser than that in fresh stem extracts (FSE) 186 µg/g. All the extracts showed activity against test organisms (Staphylococcus aureus, Escherichia coli, Pseudomonas spp.) in a concentration-dependent manner. There was an observed resistance of S. aureus against FSE at 25 mg/ml. E.coli and Pseudomonas spp were sensitive to DSE at almost all concentrations Pseudomonas spp was sensitive to almost all the control drugs except cefuroxime where it recorded a resistance. DPPH radical scavenging activity was positively correlated to the concentration of the stem extracts. FSE sample showed higher DPPH radical scavenging activity than DSE as evident in Table 3 and Fig. 2. The reducing power of the extracts followed the order of DSE < FSE < VITC. ABTS radical scavenging activity was also positively correlated to the concentrations of the stem extracts. In this analysis, FSE sample showed higher radical scavenging activity of ABTS than the DSE sample.

Keywords

EN

Antimicrobial; Antioxidant; Costus afer; DDPH; Flavonoids; Stem extract

Discipline

• BIOCHEMISTRY: BIOCHEMISTRY

• Publisher: Scientific Publishing House „DARWIN”
• Journal: World News of Natural Sciences
• Year: 2024
• Volume: 53
• Pages: 1-16

Contributors

author

Israel Azogor Ekoro

• Department of Chemistry, College of Sciences, Federal University of Petroleum Resources, Effurun, Delta State, Nigeria

author

Mary Olire Edema

• Department of Chemistry, College of Sciences, Federal University of Petroleum Resources, Effurun, Delta State, Nigeria

author

Christiana Ene Ogwuche

• Department of Chemistry, College of Sciences, Federal University of Petroleum Resources, Effurun, Delta State, Nigeria

References

• References
• [1] Anyasor, G.N., Matthew, O.E, Fadairo, O. Costus afer Stem Extracts Protected against Paracetamol Induced Oxidative Stress and Liver Damage in Rats. Spatula DD, 3 (4) (2013) 147-154
• [2] Balaji, K., Ni, L., Rajindran, B., Sikarwar, M., Fuloria, N. , Fuloria, S. Determination of Total Phenolic, Flavonoid Content and Antioxidant Activity of Terminalia Chebula (Fruit). Research Journal of Pharmaceutical, Biological and Chemical Sciences, 6 (2015) 413-417
• [3] Baliyan, S., Mukherjee, R., Priysdarshini, A., Vibhuti, A., Gupta, A., Pandey, R., Chang, C. Determination of Antioxidants by DDPH Radical Scavenging Activity and Quantitative Phytochemical Analysis of Fiscus religiosa. Molecules, 27 (2002) 1326
• [4] Bassey, E.E., Obi, N. Phytochemical Antimicrobial Screening of Costus afer Extract and Its Alleviation of Carbon Tetrachloride Induced Toxicity. Open Journal of Gastroenterology and Hepatology 1 (2018) 7
• [5] Daniel, I.E., Etukudo, E.D. Nutraceutical Composition and Antimicrobial Activity of Cyperus Esculentus (Tiger Nut) Against Urinary Tract Infection Pathogens. Journal of Agroalimentary Processes and Technologies, 25(3) (2019) 127-136
• [6] Daniel, I.E., Akpan, C.E., Utam, C.E. Phytochemical Evaluation, Antioxidant and Antimicrobial Activities of Various Extracts from Leaves and Stems of Bryophyllum pinnatum. Nepal Journal of Biotechnology, 8(1) (2020) 17-28
• [7] Dias, M.C., Pinto, D.C.G.A, Silva, A.M.S. Plant Flavonoids: Chemical and Biological Activity, Molecules, 26 (17) (2021) 5377
• [8] Dutta, S., Dey, P., Chaudhuri, T.K. Phytochemical investigation and correlation study of Croton bonplandianus Baill. Stem. Journal of Pharmacognosy and Phytochemistry, 3(1) (2014) 142-148
• [9] Edema, M. O., Dickson, U. J., Igbe, I. Antibacterial and Antifungal Activity and Bone Healing Potency of N-hexane Extract of Cissus arguta Hook F. Nigerian Journal of Chemical Research, 17 (2012) 1-11
• [10] González-Palma, I., Escalona-Buendía H.B., Ponce-Alquicira, E., Téllez-Téllez, M., Gupta, V.K., Díaz-Godínez, G., Soriano-Santos, J. Evaluation of the Antioxidant Activity of Aqueous and Methanol Extracts of Pleurotus ostreatus in Different Growth Stages. Front. Microbiol, 7 (2016) 1099
• [11] Gutierrez-Venegas, G., Gomez-Mora, J.A., Meraz-Rodriguez, M.A., Flores-Sanchez, M.A., Oritz-Miranda, L.F. Effects of Flavonoids on Antimicrobial activity of Microorganisms present in Dental Plaque. Heliyon 5 (2019) e03013
• [12] Herald, T.J., Gadgil, P., Tilley, M. High-throughput Micro Plate Assays for Screening Flavonoid Content and DPPH-scavenging Activity in Sorghum Bran and Flour. J. Sci. Food Agric. 92 (11) (2012) 2326-2331
• [13] Izunwanne, U., Akalazu, J., Ibe, C., Ahuchaogu, A., Amadikwa, C. Chemical Composition of the Stem Extract of Costus afer (Bush Cane) and Its Antimicrobial Activity. British Journal of Pharmaceutical Research 10 (2016) 1-9
• [14] Jimenez-alvarez, D., Giuffrida, F., Golay, P.A., Cotting, C., Lardeau, A., keely, B.J. Antioxidant Activity of Oregano, Parsley, and Olive Mill Wastewaters in Bulk Oils and Oil-in-Water Emulsions Enriched in Fish Oil, J. Agric. Food Chem. 56 (2008) 7151–7159
• [15] Johnson, M.A., Gowtham, J., Janakiraman, N., Malar, T.R.J., Rocha, J.E., Coutinho, H.D.M. Phytochemical Profile of Asplenium aethiopicum (Burm. f.) Becherer Using HPTLC. Separations 7(8) (2020)
• [16] Karak, P. Biological Activities of Flavonoids: An Overview. International Journal of Pharmaceutical Sciences and Research, 10 (4) (2018) 1567-1574
• [17] Kim, J.D., Khan, M.I., Shin, J.H., Le, M.G., Seo, H.J., Shin, S.T., Kim, M.Y. HPLC Fractionation and Pharmacological Assessment of Green Tea Seed Saponins for Antimicrobial, Anti-angiogenic and Hemolytic Activities. Biotechnology and Bioprocess Engineering, 20 (2015) 1035-1043
• [18] Liu, Y., Lu, X., Li, W., Yang, L., Zhao, Y., Chen, W. Combination of Solid-phase Extraction, Thin-layer Chromatography, and High-speed counter-Current Chromatography for Rapid Isolation of Flavonoids from Fructus sophorae. Journal of Chromatographic Science 56 (7) (2018) 625-631
• [19] Matik, M., Gasik, A. Polyphenols in Food. The Impact on Organoleptic Characteristics of Food. Przem Spoż, 5 (2009) 34-39.
• [20] Nazaj, D. Medicinal and Aromatic Plants and Their Role in Healthcare and Economic Development. International Journal of Agriculture and Biological Sciences, 1 (2017) 1-7
• [21] Nijveldt, R.J., Van Nood E., Van Hoorn, D.E., Boelens, P.G., Van Norren, K., Van Leeuwen, P.A. Flavonoids: A Review of Probable Mechanisms of Action and Potential Applications. American Journal of Clinical Nutrition, 74 (4) (2001) 418-425
• [22] Ogwuche, C.E., Amupitan, J.O. Antimicrobial Activity of the Leaf of the White Species of Sesamum indicum from Benue State, Nigeria. International Journal of Biological and Chemical Sciences, 9 (2) (2015) 996-1003
• [23] Panche, A.N., Diwan, A.D., Chandra, S.R. Flavonoid: An Overview. Journal of Nutritional Science 5 (47) (2016) 1-15
• [24] Tano, M.B., Kabran, G.R.M., Konan, M.K., Yeboue, A.K., Mamyrbekova-Bekro, J.A., Beckro, Y.A. Thin Layer Chromatographic Profiles and Antioxidant Capacity of Leaf Extracts of Nelumbo nucifera gaertn. (Nelumbonaceae) from Côte D’Ivoire. European Journal of Biomedical and Pharmaceutical Sciences, 5(12) (2018) 111-118
• [25] Zhang, Q., Zhang, J., Shen J., Silva, A., Dennis, D.A., Barrow, C.J. A Simple 96-well Microplate Method for Estimation of Total Polyphenol Content in Seaweeds. J Appl Phycol, 18 (2006) 445–450

• Document Type: article