Effect of Protein Extract of Mangifera indica Pollen on the Erythrocytes (Red Blood Cell) of Albino Rats

• Authors: R. O. Ezebo , J. N. Omumuabuike , B. G. Esimai , E. E. Ilouno , O. U. Obieze , J. N. Obienyem , C. A. Uba
• Languages of publication: EN

Abstracts

EN

This study was carried out to show the effect of the protein extract of Mangifera indica on the red blood cell (erythrocytes) of albino rats. The Mangifera protein was extracted using 500ml of 0.02m (20mM) phosphate- buffered saline (PBS), at pH 7.4, in a large beaker (stirred with a magnetic stirrer for 3 hours at room temperature). The crude extract was saturated to 60% by adding solid ammonium sulphate under constant gentle attiring, and then stored in a refrigerator for 6 hours. Sixteen male albino rats obtained from animal house in Faculty of Biological Science, University of Nigeria Nsukka was used for the study. They were divided into two groups of eight. One group was labeled the experimental group and the other control. The extract was administered to the experimental rats intra nasally for a period of seven (7) days. Data were expressed as mean ± standard error. Means were separated using Duncan’s New Multiple Range Test (DNMRT). Blood samples were collected via the orbital plexus of rats to determine the effect of the extract on red blood cell (erythrocytes). The present study demonstrated that the extracted pollen of Mangifera indica had no allergic effect on ratsand so would need to be further investigated.

Keywords

EN

Albino Rat; Duncan’s New Multiple Range Test; Erythrocytes; Mangifera indica; Pollen; Protein extract; red blood cell

Discipline

• VETERINARY_MEDICINE: VETERINARY MEDICINE

• Publisher: Scientific Publishing House „DARWIN”
• Journal: World News of Natural Sciences
• Year: 2021
• Volume: 36
• Pages: 114-124

Contributors

author

R. O. Ezebo

• Department of Botany Faculty of Bioscience, NnamdiAzikiwe University, P.M.B. 5025, Awka, Anambra State, Nigeria

author

J. N. Omumuabuike

• Department of Animal Health and Production Technology, Anambra State Polytechnic, P.M.B. 002, Mgbakwu, Anambra State, Nigeria

author

B. G. Esimai

• Department of Science Laboratory Technology, Anambra State Polytechnic, P.M.B. 002, Mgbakwu, Anambra State, Nigeria

author

E. E. Ilouno

• Department of Agricultural Technology, Anambra State Polytechnic, P.M.B. 002, Mgbakwu, Anambra State, Nigeria

author

O. U. Obieze

• Department of Science Laboratory Technology, Anambra State Polytechnic, P.M.B. 002, Mgbakwu, Anambra State, Nigeria

author

J. N. Obienyem

• Department of Animal Health and Production Technology, Anambra State Polytechnic, P.M.B. 002, Mgbakwu, Anambra State, Nigeria

author

C. A. Uba

• Department of Animal Health and Production Technology, Anambra State Polytechnic, P.M.B. 002, Mgbakwu, Anambra State, Nigeria

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• Document Type: article