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Phytochemical Profile and Antioxidant Potential of Rind Essential Oil of Citrus reticulate Blanco

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Oxidative stress occurs as a result of an imbalance between production of reactive oxygen species and the regulatory mechanism produced by human cells. The stress contributes to the pathogenesis of inflammatory diseases. Synthetic antioxidants like butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT) and ascorbic acid, are used in curtailing the menace of the stress. Their potential depends on the type of phytochemicals in the oil. It is on this basis this study aimed at isolating, characterizing and investigating antioxidant potential of rind essential oil of C. reticulata. To achieve this aim, 500g of fresh rinds of C. reticulata were pulverized and hydro-distilled for three hours. GC and GC-MS analysis of the oil revealed the abundance of hydrocarbon monoterpenoids (96%). The most abundant compounds in the oil were D-limonene (82.4%), γ-Terpinene (9.4%) and β-Myrcene (2.2%). The oil exhibited antioxidant activity, with IC50 value (10.73 μl/ml), which was lower to the activity of ascorbic acid (49.14 μl/ml). This shows that the oil is more active than ascorbic acid. Hence, the oil could be explored for the treatment of oxidative stress.
Physical description
  • Central Chemistry Laboratory, Department of Chemistry, Faculty of Physical Sciences, University of Ilorin, Tanke, 240003, Nigeria
  • Central Chemistry Laboratory, Department of Chemistry, Faculty of Physical Sciences, University of Ilorin, Tanke, 240003, Nigeria
  • [1] Anand, K.K., Akhilesh, K.V., Tarun, K.L., and Ragini, S.V. Essential Oil in Production. International Journal of Science and Techno ledge 8 (2010) 19-21
  • [2] Augustyniak, A., Grzegorz, B., Ana, C., Gunars, L., Petras, R., Jana, V., Panagiota, S., and Neven, Z. Natural and synthetic antioxidants: An updated overview. Free Radical Research 44(10) (2010) 1216-1262
  • [3] Aziz, Z., Siti, H., and Muhammad, K. Essential Oils: Extraction Techniques, Pharmaceutical and Therapeutic Potential. Current Drug Metabolism 19 (2018) 1-10
  • [4] Bakkali, F., Averbeck, S., and Idaomar, M. Biological effects of essential oils. Food and Chemical Toxicology 46 (2008) 446-475
  • [5] Buchbauer, G. The detailed analysis of essential oils leads to the understanding of their properties. Perfumer and Flavorist 25 (2000) 64-67
  • [6] Burt, S. Essential oils: their antibacterial properties and potential applications in foods. International Journal of Food Microbiology 94 (2004) 223-253
  • [7] Cengiz, S., Olcay, C., Said, T., and Sanja, K. Chemical composition and biological activities of the essential oils of two endemic Nepeta species. Industrial Crops and Products 125 (2006) 5-8
  • [8] Choi, S.P., Kang, M.Y., Koh, H.J., Nam, S.H., and Friedman, M. Anti-allergic activities of pigmented rice bran extracts in cell assays. Journal of Food Science 72(9) (2007) 719-726
  • [9] Dhanani, T., Shah, S., Gajbhiye, N., and Kumar, S. Effect of extraction methods on yield, Phytochemical constituents and antioxidant activity of Withanaiasomnifera. Arab J. Chem. 7 (2013) 4-8
  • [10] Dmytro, G., and Volodymyr, L. Oxidative Stress and Diseases: Biosynthesis and Biological Functions of Terpenoids in Plants. Advances in Biochemical Engineering/Biotechnology 2(6) (2015) 27-34
  • [11] Emad, M. Atta, M., Nawal, H., and Ahmed A. Antioxidants: An Overview on the Natural and Synthetic Types. Eur. Chem. Bull. 6(8) (2017) 365-375
  • [12] Fadel, H., Marx, F., El-Sawy, A., and El-Gorab, A. Effect of extraction techniques on the chemical composition and antioxidant activity of Eucalyptus camaldulensis. Eur. Chem. Bull. 208 (2009) 212-216
  • [13] Ferhat, M., Meklati, B., Smadja J., and Chemat, F. An improved microwave Clevenger apparatus for distillation of essential oils from orange peel. Journal of Chromatography 1112(1-2) (2006) 121-126
  • [14] Filly, A., Fernandez, M., Minuti, F., Visinoni, C., and Chemat, F. Solvent- Free microwave extraction of essential oil from aromatic herbs: from laboratory to pilot and industrial scale. Food Chem. 150 (2014) 193-198.
  • [15] Fischbach, R.J., Zimmer, W., and Schnitzler, J.P. Isolation and functional analysis of a DNA encoding a myrcene synthase from holm oak (Quercus ilex L.). Eur. J. Biochem. 268 (2001) 5633–5638.
  • [16] Fusco, D., Colloca, G., Monaco, M., and Cesari, M. Effects of antioxidant supplementation on the aging process. Clinical Interventions in Aging 2(3) (2007) 377–387
  • [17] Gupta, V., Mittal, P., Bansal, P., Khokra, S., and Kaushik, D. Pharmacological Potential of Matricaria recutita. International Journal of Pharmaceutical Sciences and Drug Research 2 (2010) 12-16
  • [18] Hamid, A.A., Aiyelaagbe, O.O., and Usman, L.A. Essential oils: its medicinal and Pharmacological uses. International Journal of Current Research 33(2) (2011) 86-98
  • [19] Hesham, H., Rassem, A., Abdul-Rahman, H., Nour, H., and Rosli, M. Techniques For Extraction of Essential Oils from Plants: A Review. Australian Journal of Basic and Applied Sciences 10(16) (2016) 117-127
  • [20] Hicham, B., and Zahra, B. Chemical Composition and Biological Activity of Essential Oil of Mandarin (Citrus reticulata) Cultivated in Algeria. Int. J. Pharm. Sci. 44(1) (2017) 179-184
  • [21] Kalita, B., Bora, S., and Sharma, A. Plant Essential Oils as Mosquito Repellent: A Review. Int. J. Res. Dev. Pharm. L. Sci. 3(1) (2013) 741-747
  • [22] Karim, A.P. Ultrasound-induced intensification and selective extraction of Essential oil from Carum carvi L. seeds. Chem. Eng. Process Intensif. 62 (2012) 99-105
  • [23] Kaufaman, B., and Christen, P. Recent extraction and pressurized solvent extraction. Phytochem. Anal. 13 (2002) 105-113
  • [24] Kishmu, L. A Review on Major Constituents of Various Essential Oils and its Application. Transl. Med. 8 (2018) 201-206
  • [25] Kollas, A.K., Duin, E.C., Eberl, M., Altincicek, B., Hintz, M., Reichenberg, A., Henschker, D., Henne, A., Steinbrecher, I., Jomaa, H., and Wiesner, J. Functional characterization of an Essential enzyme of the non-mevalonate pathway of isoprenoid biosynthesis. FEBS Lett. 532 (200) 432-436
  • [26] Kosar, M., Dorman, H., and Hiltunen, R. Effect of an acid treatment on the phytochemical and antioxidant characteristics of extracts from selected Lamiaceae species. Food Chemistry 3(91) (2005) 525-533
  • [27] Kusmita, L., Puspitaningrum, I. and Limantara, L. (2015).Identification, isolation and antioxidant activity of pheophytin from green tea (Camellia sinensis (L.) Kuntze). Procedia Chemistry Journal 14 (2015) 232-238.
  • [28] Lee, M., Gräwert, T., Quitterer, F., Rohdich, F., Eppinger, J., Eisenreich, W., Bacher, A., and Groll, M. Biosynthesis of isoprenoids: Crystal structure of the [4Fe–4S] cluster protein IspG. J. Mol. Biol. 404 (2010) 600–610.
  • [29] Letellier, M., Budzinski, L., and Charrier, S. Optimization by factorial design of focused microwave assisted extraction of polycyclic aromatic hydrocarbons from marine sediment. J. Anal. Chem. 364 (2009) 228-237
  • [30] Licina, B.Z., Stefanovic, O.D., Vasic, S.M., Radojevic, I.D., Dekic, M.S., and Comic, L.R. Biological activities of the extracts from wild growing Origanum vulgare L. Food Control 33 (2013) 498–504
  • [31] Lucchesi, M.E., Chemat, F.J., and Smadja, K. Solvent-free microwave extraction of essential oil from aromatic herbs: comparison with conventional hydro- distillation. Journal of Chromatography 1043 (2004) 323-327
  • [32] Macías, F.A., Chinchilla, N., Varela, R.M., and Molinillo, J.M. Bioactive steroids from Oryza sativa L. Steroids 71 (2006) 603-608
  • [33] Mandal, V., Mohan Y., and Hemalatha, S. Microwave-assisted extraction-An innovative and promising extraction tool for medicinal plant research. Pharmacognosy Reviews 1(1) (2007) 2-6
  • [34] Martín, A., Varona, S., Navarrete, A., and Cocero, M.J. Encapsulation and Co-Precipitation Processes with Supercritical Fluids: Applications with Essential Oils. The Open Chemical Engineering Journal 4 (2010) 31-41
  • [35] Mayaud, L., Carricajo, A., Zhiri, G. Comparison of bacteriostatic and bactericidal activity of 13 essential oils against strains with varying sensitivity to antibiotics. Lett. Appl. Microbiol. 47 (2008) 167-173
  • [36] Misra, G., Pavlostathis, S., Perdue, E., and Araujo, R. Aerobic biodegradation of selected monoterpenes. Appl. Microbiol. Biotechnol. 45 (1996) 831-838
  • [37] Oussalah, M., Caillet, L., Saucier, L., and Lacroix, M. Antimicrobial effects of selected plant essential oils on the growth of a Pseudomonas putida strain isolated from meat. Meat Sci. 73 (2006) 236-244
  • [38] Ozkan, M. Glandular hairs of Salvia recognita (Lamiaceae) in Turkey. Bangladesh Journal of Botany 37 (2008) 93-95
  • [39] Parvin, R., Shahroh, O., Mozafar, S., Hassan, E., and Mehrdad, B. Biosynthesis, regulation and properties of plant monoterpenoids. Journal of Medicinal Plant Research 8(29) (2014) 983-991
  • [40] Said, M.A., and Aiman, I.A. Oxidative stress versus antioxidants. American Journal of Bioscience and Bioengineering 2(5) (2014) 60-71
  • [41] Sato, M., Goto, T., and Hirose, T. Supercritical fluid extraction on semi batch mode for the removal of terpenes in citrus oils. J. Ind. Eng. Chem. Res. 35 (1996) 1906-1911
  • [42] Seoussen, K., Hamama, B., Fatih, D., Ibrahim, G. Phytochemical Screening, Antioxidant and Antimicrobial Activities of Algerian Cistus Salvifolius Extracts. Advances in Environmental Biology 10(1) (2016) 23-32
  • [43] Wang, L., and Weller, C. Recent advances in extraction of nutraceuticals from plants. Trends Food Sci. Technol. 17 (2006) 300-312.
  • [44] Yang, T., Li, J., Wang, H.X., Zeng, Y., (2005). A geraniol-synthase gene from Cinnamomum tenuipilum. Phytochemistry 66 (2005) 285-293
  • [45] Yuba, A., Yazaki, K., Tabata, M., Honda, G., and Croteau, R. CDNA cloning, characterization, and functional expression of 4S-(_)-limonene synthase from Perilla frutescens. Arch. Biochem. Biophys. 332 (1996) 280-287
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