Mucuna pruriens-seed extract stimulating immune system in Litopenaeus vannamei against Vibrio harveyi

• Authors: G. Shanmugavel , G. Krishnamoorthy
• Languages of publication: EN

Abstracts

EN

Objective: To evaluate the immunostimulant (ISt) role of Mucuna pruriens-seed (Mp-seed) enriched with Artemia (Ar) in Litopenaeus vannamei (L. vannamei) infected by Vibrio harveyi (V. harveyi). Method: Fifteen shrimp (30±5g) were stocked per 250 L tank in 3 replicates and fed twice daily with Ar enriched with Mp-seed methanolic extracts at 100 ppm and 200 ppm for 21 days. Infected L. vannamei shows altered immunoparameter (IPM) and antioxidant enzyme status due to the V. harveyi infection induced stress. Results: Significantly increased (p<0.05) total haemocyte count, respiratory burst, phenoloxidase and superoxide dismutase activities were observed in shrimp fed with Mp enriched Ar diets compared to infected group. Supplementation of Mp enriched Ar in diet could enhance the immunity of shrimp against V. harveyi infection by reverses the antioxidant level. The histology of hepatopancreas (HPC) also shows Mp-fed groups has restores the normal structure. Conclusion: The study demonstrated that Mp enriched Ar in diet have a potentially role in ISt and an effective antioxidant by regulating infectious stress against vibriosis.

Keywords

EN

Litopenaeus vannamei; Mucuna pruriens; Vibrio harveyi; Vibriosis; immunostimulant

Discipline

• BIOCHEMISTRY: BIOCHEMISTRY

• Publisher: Scientific Publishing House „DARWIN”
• Journal: World News of Natural Sciences
• Year: 2021
• Volume: 39
• Pages: 95-112

Contributors

author

G. Shanmugavel

• Department of Chemsitry, Puducherry Technological University, Puducherry 605 014, India

author

G. Krishnamoorthy

• Department of Zoology, Tagore Government Arts and Science College, Puducherry 605 008, India

References

• [1] Crab R. Bioflocs technology: An integrated system for the removal of nutrients and simultaneous production of feed in aquaculture. Ghent Belgium, Grant University 2010.
• [2] Emerenciano M, Cuzon G, Arévalo M, Gaxiola G. Biofloc technology in intensive broodstock farming of the pink shrimp Farfantepenaeus duorarum: Spawning performance, biochemical composition and fatty acid profile of eggs. Aquacult Res 2013; 45: 1713-1726
• [3] Aguilera-Rivera D, Prieto-Davó A, Escalante K, Chávez C, Cuzon G, Gaxiola G. Probiotic effect of floc on Vibriosin the Pacific white shrimp Litopenaeus vannamei. Aquaculture 2014; 424: 215-219
• [4] Avnimelech Y. Biofloc technology. A practical guidebook. Baton Rouge, LA: The World Aquaculture Society, 2015
• [5] Cabello FC, Godfrey HP, Tomova A, Ivanova L, Dölz H, Millanao A et al. Antimicrobial use in aquaculture re-examined: its relevance to antimicrobial resistance and to animal and human health. Appl Environm Microbiol 2013; 15: 1917-1942
• [6] Ma D, Hu Y, Wang J, Ye S, Li A. Effects of antibacterials use in aquaculture on biogeochemical processes in marine sediment. The Science of the Total Environment 2006; 367: 273-277
• [7] Letchumanan V, Chan KG, Lee LH. An insight of traditional plasmid curing in Vibrio species. Front Microbiol 2015; 6: 735
• [8] Baranitharan M, Tamizhazhagan V, Kovendan K. Medicinal Plants as Potent Power for Malaria Control: Review. Entomol Appl Scien Lett 2019; 6: 28-44
• [9] Mathalaimuthu Baranitharan, Saud Alarifi, Saad Alkahtani, Daoud Ali, Kuppusamy Elumalai, Jeganathan Pandiyan, Kaliyamoorthy Krishnappa, Mohan Rajeswary, Marimuthu Govindarajan, Phytochemical analysis and fabrication of silver nanoparticles using Acacia catechu: An efficacious and ecofriendly control tool against selected polyphagous insect pests. Saudi Journal of Biological Sciences, Volume 28, Issue 1, 2021, Pages 148-156, https://doi.org/10.1016/j.sjbs.2020.09.024
• [10] Nonwachai T, Purivirojkul W, Limsuwan C, Chuchird N, Velasco M, Dhar AK. Growth, nonspecific immune characteristics, and survival upon challenge with Vibrio harveyi in Pacific white shrimp (Litopenaeus vannamei) raisedon diets containing algal meal. Fish and Shellfish Immunology 2010; 29(2): 298–304
• [11] Uhegbu FO, Elekwa I, Ukoha C. Comparative Efficacy of crude Aqueous Extract of Mangiferaindica, carica papaya and sulphadoxine pyrimethamine on the mice infested with malaria parasite in vivo. Global J Pure Appl Sci 2005; 399-401
• [12] Babu MM, Sivaram V, Immanuel G, Citarasu T, Punitha SMJ. Effects of herbal enriched ARM supplementation over the reproductive performance and larval quality in spent spawners of the tiger shrimp (Penaeus monodon). Turkish J Fish Aquat Sci 2008; 8: 301–307
• [13] Joseph A, Phillip R. Acute salinity stress alters the haemolymph metabolic profile of Penaeus monodon and reduces immunocompetence to white spot syndrome virus infection. Aquacult 2007; 272: 87-97
• [14] Ashida M, Soderhall K. The prophenoloxidase activating system in crayfish. Comparat Biochem Physiol B 1984; 77: 21-26
• [15] Song YL, Hsieh YT. Immunostimulation of tiger shrimp (Penaeus monodon) hemocytes for generation of microbicidal substances: analysis of reactive oxygen species. Developmen Comparat Immunol 1994; 18: 201-209
• [16] Marklund S, Marklund G. Involvement of superoxide anion radical in the auto-oxidation of pyragallol, and a convenient assay for superoxide dismutase. European J Biochem 1974; 47: 469-474
• [17] Sinha AK. Colorimetric assay of catalase. Anal Biochem 1972; 47: 389-94
• [18] Rotruck JT, Pope AL, Ganther HE, Swanson AB, Hafeman DG, Hoekstra WG. Selenium: biochemical role as component of glutathione peroxidase. Science 1973; 179: 588-590.
• [19] Staal GEJ, Visser J, Veeger C. Purification and properties of glutathione reductase of human erythrocytes. Biochimicaet Biophysica Acta 1969; 185: 39-48
• [20] Bell TA, Lightner DV. A Handbook of Normal Penaeid Shrimp Histology. World Aquacult Societ, Baton Rouge, LA, USA 1988.
• [21] Sanchez-Paz A. White spot syndrome virus: an overview on an emergent concern. Veterinary Research, 2010, 41: 43. DOI: 10.1051/vetres/2010015
• [22] Al-Dabbagh B, Elhaty IA, Murali C, Al Madhoon A, Amin A. Salvadora persica (miswak): antioxidant and promising anti angiogenic insights. American Journal of Plant Science, 2018, 09: 1228–1244. DOI: 10.4236/ajps.2018.96091
• [23] Mohamed AL, Waleed NE, Ramy MS, Ali HE, Ramadan SS, Shaker AM. Miswak (Salvadora persica) dietary supplementation improves antioxidant status and nonspecific immunity in Nile tilapia (Oreochromis niloticus). Fish Shellfis Immunol 2019; 88: 619-626. DOI: 10.1016/j.fsi.2019.03.037
• [24] Vibin A, Sivagnanavelmurugan M, Ramasubburayan R, Palavesam A, Immanuel G. Immunostimulatory effect and propo gene expression status of methanolic extract of Phyllanthus niruri against WSSV in shrimp Penaeus monodon (Fab). J Aquat Biol Fisher 2020; 8: 26-36
• [25] Van de Braak K. Hemocytic defense in black tiger shrimp (Penaeus monodon). Doctor degree thesis. Wageningen Institute of Animal Science. Wageningen University. The Netherlands 2002.
• [26] Dinamella W, Teuku RE, Kustiariyah T, Munti Y, Irzal E, Fazril S. Supplementation of Nodulisporium sp. KT29 induced by Vibrio harveyi as an immunostimulant for controlling vibriosis in vannamei white shrimp under marine culture system. Jurnal Akuakultur Indonesia 2020; 19(2): 95-105
• [27] Amparyup P, Charoensapsri W, Tassanakajon A. Prophenoloxidase system and its role in shrimp immune responses against major pathogens. Fish Shellfi Immunol 2013; 34: 990-1001
• [28] Li F, Xiang J. Recent advances in researches on the innate immunity of shrimp in China [Review]. Development Comparat Immunol 2013; 39: 11-26
• [29] Ince Ayu K Kadriah, Kamaruddin and Nurhidayah. Survival of Penaeus monodon shrimp fed diets supplemented with extract Porphyridium aerugineum and Porphyridium sp. after challenges by Vibrio harveyi. Earth Environmen Sci 2020; 564: 012055.
• [30] Tassanakajon A, Somboonwiwat K, Supungul P, Tang S. Discovery of immune molecules and their crucial functions in shrimp immunity. Fish Shellfish Immunol 2013; 34: 954-967
• [31] Risjani, Y.; Mutmainnah, N.; Wulan, S.N.; Yunianta, Y. Extracellular Polysaccharide from Porphyridium cruentum (purpureum) Stimulates the Non-Specific Immune Activities on White Shrimp (Litopenaeus vannamei). Preprints 2020, 2020120193, doi: 10.20944/preprints202012.0193.v1
• [32] El-Desouky H, El-Asely A, Shaheen AA, Abbass A. Effects of Zingiber officinalis and Cynodon dactylon on the growth performance and immune parameters of Macrobrachium rosenbergii. World J Fish Marin Sci 2012; 4: 301-307
• [33] Nindarwi D, Yanuhar D, Sukoso U. Non-specific immune response of Litopenaeus vannamei shrimp induced by chitosan as an immunomodulator against IMNV (infectious myonecrosis virus) exposure. J Biol Life Sci 2013; 4: 320-327
• [34] Chen YH, He JG. Effects of environmental stress on shrimp innate immunity and white spot syndrome virus infection. Fish Shellfish Immunol 2019; 84: 744-755. https://doi.org/10.1016/j.fsi.2018.10.069
• [35] Liu F, Li S, Yu Y, Sun M, Xiang J, Li F. Effects of ammonia stress on the hemocytes of the pacific white shrimp Litopenaeus vannamei. Chemosphere, 2020; 239: 1-12. https://doi.org/10.1016/j.chemosphere.2019.124759
• [36] Sivagnanavelmurugan M, Thaddaeus BJ, Palavesam A, Immanuel G. Dietary effect of Sargassum wightii fucoidan to enhance growth, prophenoloxidase gene expression of Penaeus monodon, and Immune resistance to Vibrio parahaemolyticus. Fish Shellfish Immunol 2014; 39(2): 429-449. https://doi.org/10.1016/j.fsi.2014.05.037
• [37] Saputra I, Fotedar R, Gupta SK, Siddik MAB, Fosyal MJ. Effects of different dietary protein sources on the immunological and physiological responses of marron, Cherax cainii (Austin and Raynn, 2002), and Its susceptibility to high temperature exposure. Fish Shellfish Immunol 2019; 88: 567-577. https://doi.org/10.1016/j.fsi.2019.03.012
• [38] Chen Su, Depeng Fan, Luqing Pan, Yusong Lu, Yuxuan Wang, Mengyu Zhang. Effects of Yu-Ping-Feng polysaccharides (YPS) on the immune response, intestinal microbiota, disease resistance and growth performance of Litopenaeus vannamei. Fish Shellfish Immunol 2020; 105: 104-116
• [39] Declarador RS, Serrano JrAE, Corre JrVL. Ulvan extract acts as immunostimulant against white spot syndrome virus (WSSV) in juvenile black tiger shrimp Penaeus monodon. AACL Bioflux 2014; 7: 153-161
• [40] Aladaileh S, Nair VS, Birch D, Raftos AD. Sydney rock oyster (Saccostrea glomerata) hemocytes: Morphology and function. J Inverteb Pathol 2007; 96: 48-63
• [41] Sarjito, Haditomo AHC, Erlinda K, Desrina, Prayitno SB. Role of Gracilaria verrucosa extract in the feed as Immunostimulant of white shrimp (Litopenaeus vannamei) infected Vibrio harveyi. Adv Anim Vet Sci 2020; 8: 1427-1434
• [42] Beula Shiny D, Vijila Jasmin J, S. Sujithra, M. Jenifer Tamizharasi and T. Kumaran. Phytochemical screening and immunostimulant efficiency of selected medicinal plant extracts against white spot syndrome virus. Plant Archives 2020; 20(2): 7021-7026
• [43] Jasmanindar Y, Sukenda S, Jr. Zairin M, Alimuddin A, Utomo NBP. Dietary administration of Gracilaria verrucosa extract on Litopenaeus vannamei immune response, growth, and resistance to Vibrio harveyi. AACL Bioflux. 2018; 11: 1069-1080
• [44] Liu PC, Lin PW, Huang CL, Hsu CH, Chen JC. Long-term administration of diets containing Gracilaria tenuistipitata extract induce the expression of immune related genes and increase the immune response and resistance against Vibrio harveyi in White Shrimp Litopenaeus vannamei. Gene Rep 2019; 15: 1-9. https://doi.org/10.1016/j.genrep.2019.100378
• [45] Kumaran T, Thirumalaikumar E, Lelin C, Palanikumar P, Michaelbabu M, Citarasu T. Physicochemical properties of anti-Vibrio harveyi egg yolk antibody (IgY) and its immunological influence in Indian white shrimp Fenneropenaeus indicus. Fish Shellfish Immunol 2018; 74: 349-362
• [46] Balasubramanian G, Sarathi M, Venkatesan C, Thomas J, Hameed ASS. Studies on the immunomodulatory effect of extract of Cynodondactylon in shrimp, Penaeus monodon, and its efficacy to protect the shrimp from white spot syndrome virus (WSSV). Fish Shellfish Immunol 2008; 25: 820-828
• [47] Livingstone DR, Lips F, Martinez PG, Pipe RK. Antioxidant enzymes in the digestive gland of the common mussel (Mytilus edulis). Marine Biol 1992; 112: 265-276
• [48] Yang CC, Lu CL, Chen S, Liao WL, Chen SN. Immune gene expression for diverse haemocytes derived from pacific white shrimp, Litopenaeus vannamei. Fish Shellfish Immunol 2015; 44: 265-271. https://doi.org/10.1016/j.fsi.2015.02.001
• [49] Rudi M, Sukenda, Wahjuningrum D, Pasaribu W, Hidayatullah D. Seaweed extract of Gracilaria verrucosa as an antibacterial and treatment against Vibrio harveyi infection of Litopenaeus vannamei. J Akuakultur Indonesia 2019; 18: 120-129. https://doi.org/10.19027/jai.18.2.11-20
• [50] Liu CH, Cheng W, Kuo CM, Chen JC. Molecular cloning and characterization of a cell adhesion molecule, peroxinectin from the white shrimp Litopenaeus vannamei. Fish and Shellfish Immunol 2004; 17: 13-26
• [51] Peixoto, M.J., Magnoni, L., Gonçalves, J.F.M. et al. Effects of dietary supplementation of Gracilaria sp. extracts on fillet quality, oxidative stress, and immune responses in European seabass (Dicentrarchus labrax). J Appl Phycol 31, 761–770 (2019). https://doi.org/10.1007/s10811-018-1519-x
• [52] Cantelli L, Goncalves P, Guertler C, Kayser M, Pilloto MR, Barracco MA, Perazzolo LM. Dietary supplementation with sulfated polysaccharides from Gracilaria birdiae promotes a delayed immunostimulation in marine shrimp challenged by the white spot syndrome virus. Aquacult Int 2019; 27: 349-367. https://doi.org/10.1007/s10499-018-0328-1
• [53] Trafalgar RFM, Corre VL, Serrano AE. Efficiency of dietary immunostimulants to enhance the immunological responses and vibriosis resistance of juvenile Penaeus monodon. J Fish Aquat Sci 2013; 8(2): 340-354. https://doi.org/10.3923/jfas.2013.340.354
• [54] Zahra A, Sukenda S, Wahjuningrum D. Extract of seaweed Gracilaria verrucosa as immunostimulant to controlling white spot disease in pacific white shrimp Litopenaeus vannamei. J Akuakultur Indonesia 2017; 16: 174-183. https://doi.org/10.19027/jai.16.2.174-183
• [55] Jasmanindar Y, Sukenda S, Alimuddin A, Junior MZ, Utomo NBP. The chemical composition of Gracilaria verrucosa extract and its utilization on survival and growth of Litopenaeus vannamei. Omni-Akuatika. 2018; 14(3), 1-9. https://doi.org/10.20884/1.oa.2018.14.3.508
• [56] Qin Z, Babu VS, Lin H, Dai Y, Kou H, Chen L, Li J, Zhao L, Lin L. The immune function of prophenoloxidase from red swamp crayfish (Procambarus clarkii) in response to bacterial infection. Fish Shellfish Immunol 2019; 92: 83-90. https://doi.org/10.1016/j.fsi.2019.05.005
• [57] Ambipillai L, Sobhana KS, George KC, Sanil NK. Histopathological survey of cultured shrimps in Cochin, Kerala. J Marine Biol Associat India 2003; 45(2): 178-185
• [58] Sarjito NE, Ningrum W, Radjasa OK, Prayitno SB. Application of repetitive sequence-based pcr on the richness of vibrio on the tiger shrimp (Penaeus monodon Fab.). J Coastal Dev 2012; 15: 303-309
• [59] Sarjito AH, Haditomo C, Desrina, Djunaedi A, Prayitno SB. The diversity of vibrios associated with vibriosis in pacific white shrimp (Litopenaeus vannamei) from extensive shrimp pond in Kendal District, Indonesia. IOP Conf. Ser. Earth Environ. Sci 2018; 116: 1-7. https://doi.org/10.1088/1755-1315/116/1/012011
• [60] Rajikkannu Muthaiyan, S. Krishnan, Selvakumar Periyasamy, Zhievino Chetri, R. Nandakumar, Immune response of shrimp Peneaus monodon against Vibrio parahaemolyticus. World News of Natural Sciences 30(2) (2020) 150-168
• [61] Rachmawati R. Andieny, Yuniar Mulyani, Emma Rochima, Roffi Grandiosa, The effect of induction of bacteria Bacillus subtilis in feed on the immune system of carp (Cyprinus carpio Linnaeus, 1758). World Scientific News 160 (2021) 203-216
• [62] Fadilah Amelia, Ayi Yustiati, Yuli Andriani, Review of Shrimp (Litopenaeus vannamei (Boone, 1931)) Farming in Indonesia: Management Operating and Development. World Scientific News 158 (2021) 145-158

• Document Type: article