Determination of fungal contamination of retailed smoked-fish sold within Owerri metropolis, Imo State, Nigeria

• Authors: P. A. Nnagbo
• Languages of publication: EN

Abstracts

EN

The consumption of smoked-fish usually from the open shelf in most communities of the developing countries has raised some health related concerns. As one of the common sources of protein available to man, fish is highly consumed due to its lower cholesterol content and price. So it forms a rich protein source for both poor and rich. This research enumerated the fungal load of retailed smoked-fish sold within Owerri metropolis, Imo State, Nigeria. A total of thirty six fish samples which comprised nine fish species such as Ethmalosa fimbrata (Bonga fish), Clarias garepinus (Mud cat fish), Ilisha Africana (West African shad) Alestes nurse (Silverside fish), Chrysichthys nigrodigitatus (Silver catfish), Gymnallabes typus (Catfish), Calamoichthys calabaricus (Rope fish), Schilbe uranoscopus (Butter fish) and Cynoglossus browni (Sole) were randomly purchased from markets in Owerri. The fish samples were inoculated onto Sabouraud dextrose agar plates incorporated with 0.5mg/ml of chloramphenicol. The fungal isolates were identified using standard methods Thirty four fungal organisms made up of four genera: Aspergillus species, Penicillium species, Fusarium species and Rhizopus species were isolated from the fish samples. Aspergillus flavus 8 (23.53%), Aspergillus fumigatus 7 (20.58%) and Rhizopus species 7 (20.58%) were the dominant mycofloral in decreasing sequential order. Aspergillus niger, Fusarium species and Penicillium species occurred in less frequency 4 (11.77%). Aspergillus flavus was the most dominant mycofloral. Aspergillus species are known to produce aflatoxins which are carcinogenic (causing hepatoma-cancer of the liver), acute hepatitis, reduced red blood cell and decreased immune system in man. Fusarium species was reported to produce fumonisin toxin and Penicillum produces penicillic acid. Prolonged intake of smoked-fish with these metabolites may constitute potential public health hazard. It is recommended that smoked-fishes be processed, stored and distributed under safe hygienic condition and good sanitary practices in order to prevent fungal contamination.

Keywords

EN

aflatoxins; carcinogenic; fumonisin toxin; penicillic acid; public health; smoked-fish

Discipline

• MICROBIOLOGY: MICROBIOLOGY

• Publisher: Scientific Publishing House „DARWIN”
• Journal: World News of Natural Sciences
• Year: 2023
• Volume: 50
• Pages: 121-129

Contributors

author

P. A. Nnagbo

• Department of Microbiology, Faculty of Biological Science, Imo State University, Owerri, Imo State, Nigeria

References

• [1] Akande, G.R. and Tobor, J.G. (1992). Improved utilization and increased availability of fishing products as an effective control of aggravated animal protein deficiency induced malnutrition in Nigeria. Proceeding of the 10th Annual Conference of Fisheries Society of Nigeria, pp. 18-31.
• [2] Adeleye, O.A. (2000). Conservation needs of fish resources and reorientation for sustainable captive and culture practices. Proceeding of the 10th Annual Conference of Fisheries Society of Nigeria, pp. 230-234.
• [3] Fagade, S.O. (2000). Keynote address on production, utilization and marketing in fisheries status and opportunities. Proceedings of the 10th Annual Conference of Fisheries Society of Nigeria. pp. 8-13.
• [4] Food Standard Agency (2004). Deterioration of oily fish. For: Food Safety and Applied Nutrition. 4: 12-15.
• [5] Ward, A.R. (1995). Fish smoking in the tropics. A review. Tropical Science 35, 103-112
• [6] Hungerford, L., Campbell, C. and Smith, A. (1998). Common Opportunistic or Contaminant Fungi. In: Veterinary Mycology Laboratory Manual, Iowa State University Press, Iowa, 58-60.
• [7] Swaminathan, B. and Sparling, P. (1998). The Bacteriology of Food Excluding Dairy Products. In: Collier, L., Balows, A. and Sussman, M., Eds., Microbiology and Microbial Infection, 9th Edition, Edward Arnold Publishers Ltd., London, Vol. 2, 407-408.
• [8] Sani, F.M., Nasir, I.A. and Torhile, G. (2016). Mycological Evaluation of Smoked-Dried Fish Sold at Maiduguri Metropolis, Nigeria: Preliminary Findings and Potential Health Implications. European Journal of Health Sciences, 2: 5-10
• [9] Tournas, V., Stack, M., Mislivec, P., Koch, H. and Bandler, R. (2001). Yeasts, Molds and Mycotoxins Mycological Analysis Manual. Food and Drug Administration, USA.
• [10] Samson, R., Houbraken, J., Thrane, U., Frisvad, J.C. and Anderson, B. (2010). Food and Indoor Fungi. CBS-KNAW Fungi Biodiversity Centre, Utrecht, The Netherlands.
• [11] Cheesbrough, M. (2000). District Laboratory Practice in Tropical Countries; Part 2. United Kingdom, Cambridge University Press, Edinburgh. Pp 64-66, 70.
• [12] Ellis, D., Davis, S., Alexiou, H., Handke, R. and Bartley, R. (2007). Description of Medical Fungi. Mycology Unit, Women and Children’s Hospital, North Adelaide, USA.
• [13] Job, M.O., Agina, S.E. and Dapiya, H.S. (2016). Occurrence of Aflatoxigenic Fungi in Smoke-dried Fish Sold in Jos Metropolis. British Microbiology Research Journal, 11: 1-7
• [14] Ayuba, V.O., Alhassan, M. and Jimmy, U. (2013). Assessment of the Microbial Load of Smoked Sardine (Sardina pilchardus, Walbaum, 1792) Sold in Makurdi Markets. International Journal of Current Microbiology and Applied Sciences, 2: 277-287
• [15] Oyebamiji, F. and Oyebimpe, F. (2013). Microbial Identification of Smoke-Dried Fish (Clarias gariepinus) from Some Local Markets in Ibadan Metropolis. Wudpecker Journal of Agricultural Research, 2: 294-298
• [16] Bukola, C.A.-T., Abiodun, A.O. and Ukpe, G.P. (2008). Mycofloral of Smoke-Dried Fishes Sold in Uyo, Eastern Nigeria. World Journal of Agricultural Sciences, 4: 346-350
• [17] Osibona, A.O., Ogunyebi, O.O. and Samuel, T.O. (2018). Storage Fungi and Mycotoxins Associated with Stored Smoked Catfish (Clarias gariepinus). Journal of Applied Sciences and Environmental Management, 22: 643-646
• [18] Shewan, J.M. (2000). The Microbiology of Sea Water Fish. In: Fish as Food. Ed: G. Borgstrom,.1 New York Academic Press. Pp. 487.
• [19] Basti, A.A., Misaghi, T. Z. and Kanikar, A. (2006). Bacteria pathogens in fresh smoked and salted Iranian fish. Food Control. 17(3), 183-188
• [20] da Silva, L.V.A., (2002). Hazard Analysis Critical Control Point (HACCP), Microbial Safety and Shelf- Life of Smoked Blue Cat Fish. Graduate Faculty of the Louisiana State University and Agricultural and Medical College, U.S.A.
• [21] Eyo, A. (2012) Traditional and Improved Fish Handling, Preservation and Processing Techniques. NAERLS/NIFER National Workshop on Fish Process Storage, Marketing and Utilization, 15-20.
• [22] [22] Essien, J.O., Ekpo, M.A., and Brooks, G.F. (2005). Mycotoxigenic and proteolytic potential of moulds associated with smoked shark fish (Clmydoselachus auguincus). J. App. Sci. Enviro. Management 9(3), 53-57.
• [23] Junaid, S.A., Olarubofin, F. and Olabode, A.O. (2010). Mycotic Contamination of Stockfish Sold in Jos, Nigeria. Journal of Yeast and Fungal Research, 1, 136-141
• [24] Edema, M. and Agbon, A. (2010). Significance of Fungi Associated with Smoke-Cured Ethmalosa fimbriata and Clarias gariepinus. Journal of Food Processing and Preservation, 34, 355-363
• [25] Ayuba, V., Onah, E. and Ataguba, G. (2012). Microbial Load on the African Catfish (Clarias gariepinus) sold in Makurdi Metropolis: A Case Study of Selected Markets. 26th Annual Conference of the Fisheries Society of Nigeria, Minna, 16-24.
• [26] Jimoh, W., Ayeloja, A., Oladele-Bukola, M., Adebayo, M., Azeez, A. and Salami, S. (2014) Isolation of Fungi Infesting Smoked African Catfish from Markets in Ibadan, Nigeria. Nigerian Journal of Fisheries and Aquaculture, 2, 13-17
• [27] Achmad Rizal, Izza M. Apriliani, Lantun P. Dewanti, Effect of Additional Soybean Oil in Feed Commercial on Pangasius djambal Bleeker, 1846 for Growth, Feed Efficiency, and Survival Rate. World Scientific News 172 (2022) 236-250
• [28] Achmad Rizal, Izza M. Apriliani, Lantun P. Dewanti, Heti Herawati. The Effect of Adding Fermented Coconut Husk Extract in Feed on The Growth Rate of Tilapia Fish (Oreochromis mossambicus (Peters, 1852)). World Scientific News 172 (2022) 282-295
• [29] Grinsya Brylian Krisyawensya, Kharisma Supriadi, Izza Mahdiana Apriliani, Identification of Vessel and Fishing Gear in Fish Landing Place (PPI) Cikidang, Pangandaran, Indonesia. World Scientific News 178 (2023) 23-43
• [30] Rollin Devasa, Silmi Biladyah Azzahra, Ainun Nahar, Annisa Maysabila, Fahira Adisti Adiara, Nora Akbarsyah, Analysis of Maximum Sustainable Yield (MSY) and Catch Per Unit Effort (CPUE) Multi Fishing Gear in PP Cikidang, Pangandaran Regency, West Java, Indonesia. World Scientific News 180 (2023) 14-24
• [31] Rizka Zahra Utami, Izza M. Apriliani, Firman G. Abdurrahman, Carp Nursery Technique (Cyprinus carpio Linnaeus, 1758) at the Regional Technical Implementation Unit (UPTD) of the Fish Seed Center (BBI) Cimaja, Sukabumi Regency, Indonesia. World Scientific News 180 (2023) 25-37
• [32] Junianto, Helmalia Asri, Veronica, Zulfiqor Meetrand, Andrean Alief Musthopa, Fajar Dwi Noeryana, Processing and organoleptic quality of salted kadukang fish (Hexanematichthys sagor (Hamilton, 1822)) in Pangandaran Regency, West Java, Indonesia. World Scientific News 180 (2023) 38-49
• [33] Reinaldy Firdaus, Lantun Paradhita Dewanti, Ai Sumiasih, Review of Trammel Net Fishing Activities as Traditional Fishing Gear That Used by Fisherman at Pangandaran Fish Landing Place, Indonesia. World Scientific News 167 (2022) 53-68

• Document Type: article