Tricholoma equestre species as a source of indole compounds and zinc released into artificial digestive juices

• Authors: Katarzyna KAŁA , Katarzyna SUŁKOWSKA-ZIAJA , Jacek ROJOWSKI , Włodzimierz OPOKA , Bożena MUSZYŃSKA
• Languages of publication: EN

Abstracts

EN

The research goals of presented work consisted of determination of zinc and indole compounds that are released into artificial digestive juices from Tricholoma equestre species. During the experiment freeze-dried samples of wild growing fruiting bodies and mycelia from in vitro cultures from liquid Oddoux medium were extracted to artificial digestive juices (saliva, gastric juice and intestinal juice). In the next step the determination of examined compounds was done by RP-HPLC for indole compounds and DP ASV for zinc. Furthermore it was decided to check weather this species has any beneficial properties for health. The extraction of researched material in conditions imitating human digestive tract allows determination of true amounts of elements released to artificial digestive juices and their beneficial influence (a specially designed and constructed apparatus Gastroel-2014 was applied). The indole compound with the highest quantity was 5-hydroxy-L-tryptophan, both in fruiting bodies and in biomass from in vitro cultures of T. equestre (up to 352.47 mg/100 g d.w.). Serotonin and L-tryptophan was determined in all analysed samples, but their amounts were significantly lower than the ones found for 5-hydroxy-L-tryptophan. The amounts of zinc on the other hand showed that the biggest concentration of zinc can be obtained for fruiting bodies and biomass from in vitro cultures to both artificial saliva and gastric juice after 120 minutes of digestion (6.83 14.4 mg/100 g d.w. retrospectively) in conditions that imitate human digestive track.

Keywords

EN

"artificial digestive juices"; "fruiting bodies; "in vitro culture"; "zinc"; Tricholoma equestre"; indole compounds"

Discipline

• BOTANY: BOTANY

• Publisher: ZOZ Ośrodek UMEA SHINODA-KURACEJO
• Journal: Medicina Internacia Revuo
• Year: 2016
• Volume: 27
• Issue: 106
• Pages: 35-39

Contributors

author

Katarzyna KAŁA

• Department of Pharmaceutical Botany, Faculty of Pharmacy, Jagiellonian University, Medical College, Medyczna 9 St., 30688 Cracow, Poland

author

Katarzyna SUŁKOWSKA-ZIAJA

• Department of Pharmaceutical Botany, Faculty of Pharmacy, Jagiellonian University, Medical College, Medyczna 9 St., 30688 Cracow, Poland

author

Jacek ROJOWSKI

• Department of Inorganic and Analytical Chemistry, Faculty of Pharmacy, Jagiellonian University, Medical College, Medyczna 9 St., 30688 Cracow, Poland

author

Włodzimierz OPOKA

• Department of Inorganic and Analytical Chemistry, Faculty of Pharmacy, Jagiellonian University, Medical College, Medyczna 9 St., 30688 Cracow, Poland

author

Bożena MUSZYŃSKA

• Department of Pharmaceutical Botany, Faculty of Pharmacy, Jagiellonian University, Medical College, Medyczna 9 St., 30688 Cracow, Poland

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• Document Type: article