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2018 | 23 | 45 - 54
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The aim of this publication is to facilitate the estimation of chitosan molecular weight (MW) in laboratories with no access to sophisticated analytical instruments, by applying the easily accessible and economical capillary viscometry. The procedure of viscosity-average molecular weight (Mv) determination is described in details. The examples provided encompass testing of the experimental procedure for determination of the Mv of chitosan with a low-molecular weight of 7.7 kDa and 88 kDa, after verification with a high-molecular weight polymer (477 kDa). The experimental work demonstrated the importance of the initial concentration of low-MW chitosan for the accurate determination of intrinsic viscosity and, as a consequence, the viscosity-average molecular weight.
45 - 54
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  • Institute of Applied Radiation Chemistry, Faculty of Chemistry Lodz University of Technology
  • Institute of Applied Radiation Chemistry, Faculty of Chemistry Lodz University of Technology
  • Institute of Applied Radiation Chemistry, Faculty of Chemistry Lodz University of Technology
  • Institute of Applied Radiation Chemistry, Faculty of Chemistry Lodz University of Technology
  • [1] S.N. Kulikov, S.A. Lisovskaya, P.V. Zelenikhin, E.A. Bezrodnykh, D.R. Shakirova, I.V. Blagodatskikh, V.E. Tikhonov (2014) Antifungal activity of oligochitosans (short chain chitosan) against some Candida species and clinical isolates of Candida albicans: Molecular weight-activity relationship, European Journal of Medical Chemistry, 74, 169-178.
  • [2] N. Liu, X.G. Chen, H.J. Park, C.G. Liu, C.S. Liu, X.H. Meng, L.J. Yu (2006) Effect of MW and concentration of chitosan on antibacterial activity of Escherichia coli, Carbohydrate Polymers, 64, 60-65.
  • [3] M. Malerba, R. Cerana (2016) Chitosan effects on plant systems, International Journal of Molecular Sciences, 17(7), article number 996.
  • [4] R. Czechowska-Biskup, D. Jarosińska, B. Rokita, P. Ulański, J.M. Rosiak (2012) Determination of degree of deacetylation of chitosan - comparison of methods, Progress on Chemistry and Application of Chitin and Its Applications, Volume XVII, 2012, 5-20.
  • [5] J.M. Rosiak, P. Ulanski, S. Al-Assaf (2009) Protocol for determination of intrinsic viscosity of chitosan, IAEA Co-ordinated Research Programme: Development of Radiation-Processed Products of Natural Polymers for Application in Agriculture, Healthcare, Industry and Environment; International Atomic Energy Agency: Research Coordination Meeting, Reims, France, 201-221.
  • [6] A. Wojtasz-Pajak, I. Kolodziejska, A. Debogorska, M. Malesa-Ciecwierz; (1998) Enzymatic, physical and chemical modification of krill chitin. Bull Sea Fish Inst 143, 29-39.
  • [7] M. Mucha (2010) Chitozan wszechstronny polimer ze źródeł odnawialnych. Wydawnictwo Naukowo-Techniczne, Warszawa.
  • [8] M.R. Kasaai (2007) Calculation of Mark-Houwink-Sakurada (MHS) equation viscometric constants for chitosan in any solvent-temperature system using experimental reported viscometric constants data, Carbohydrate Polymers, 68, 477-488.
  • [9] G. Lamarque, J.M. Lucas, C. Viton, A. Domard (2005) Physicochemical behavior of homogenous series of acetylated chitosans in aqueous solution: role of various structural parameters, Biomacromolecules, 6(1), 131-142.
  • [10] M.H. Ottøy, K.M. Vårum, B.E. Christensen, M.W. Anthonsen, O Smidsrød, (1996) Preparative and analytical size-exclusion chromatography of chitosan, Carbohydrate Polymers, 31, 253-261.
  • [11] W.M. Kulicke, C. Clasen, (2004) Viscosimetry of polymer and polyelectrolytes. Springer, Berlin.
  • [12] M.L, Huggins, (1942) Theory of solutions for high polymers, Journal American of Chemical Society, 64, 2716.
  • [13] E.O., Kraemer, (1938), Molecular Weights of Celluloses and Cellulose Derivates, Industrial Engineering Chemistry, 30, 1200.
  • [14] C.N. Costa, V.G. Teixeira, M.C. Delpech, J.V.S. Souza, M.A.S. Costa, (2015) Viscometric study of chitosan solution in acetic/sodium acetate and acetic/sodium chloride, Carbohydrate Polymers, 133, 245-250.
  • [15] A.P. Mahapatra, R.K. Samal, R.N. Samal, G.S. Roy, (2001) Evaluation of Huggins’ constant Kramers’s constant and viscosity concentration coefficient of polymer dextran in urea, glycine and glucose, Physics and Chemistry of Liquids, 39, 169-181.
  • [16] Renata Czechowska-Biskup, “Examination of the physicochemical properties of chitosan and its usability for application as a food additive” - doctoral dissertation, Lodz 2008.
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