Assessment of single and combined administration of herbal extracts on the hepatorenal system of diabetic rats

• Authors: Kingsley Chibueze Nwaegbu , Adaeze Bob Chile-Agada
• Languages of publication: EN

Abstracts

EN

This work aimed at evaluating the hepatorenal changes that occur in diabetic rats after single and combined administration of ethanol leaf extracts of Ocimum gratissimum and Kalanchoe pinnata. Thirty six (36) male adult rats was divided into six (6) groups of six (6) rats each. The group 1 was normal rats that were given only water and normal rat chow; Group 2 was untreated diabetic (DM) rats; Group 3 was DM rats that were treated with 500 mg/kg b.w. of the antidiabetic drug, metformin; Groups 4, 5 and 6 were DM rats given 200 mg/kg-1 b.w. of O. gratissimum leaves, K. pinnata leaves, and combined dose (ratio: 1:1 w/w) of the leaves of O. gratissimum + K. pinnata respectively. The rats were induced with diabetes mellitus (DM) through single intra-peritoneal (i.p.) injection of 150 mg/kg b.w. of alloxan monohydrate. Standard spectrophotometric methods were used to measure hepatorenal biomarkers and for histopathological studies. Serum ALT, AST and total bilirubin concentrations were significantly elevated (p < 0.05) while serum urea, creatinine, Na+ and K+ concentrations were significantly lowered (p < 0.05) in the rat groups treated with single and combinatorial herbal formulations (SCHF) in comparism with the untreated diabetic rat group. Histologic markers of hepatic injury and good therapeutic renal state were recorded in this study in rats treated with both SCHF. In conclusion, SCHF reversed the damages elicited on the kidney by diabetes but was not able to reverse the damage that was incurred on the liver.

Keywords

EN

diabetes; herbal formulation; histology; kidney; liver

Discipline

• BIOCHEMISTRY: BIOCHEMISTRY

• Publisher: Scientific Publishing House „DARWIN”
• Journal: World News of Natural Sciences
• Year: 2025
• Volume: 62
• Pages: 60-77

Contributors

author

Kingsley Chibueze Nwaegbu

• Department of Biochemistry, Imo State University, Owerri, Nigeria

author

Adaeze Bob Chile-Agada

• Department of Biochemistry, Imo State University, Owerri, Nigeria

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• Document Type: article