Isolation of DNA from bone material of selected animals from Cervidae family
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Bone material is one of the most difficult biological samples used for extraction of DNA. The primary objective of molecular tests is to determine the concentration and quality of DNA, and these results are of significant importance in further analyses. The aim of the study was to evaluate the usefulness of a method for DNA isolation from bone material of selected animal species from Cervidae family using a commercially available GeneMatrix Bone DNA Purification Kit manufactured by Eurx. Quantitative evaluation was performed with a spectrophotometer. Pure DNA isolates were obtained from 21 out of 24 bone fragments. The efficiency of the applied isolation method varied. The difference between the lowest and highest DNA concentration was more than hundredfold. Qualitative analysis was carried out by means of electrophoresis in 1% agarose gel. No high molecular mass DNA was obtained. The genetic material was present in small quantity and it was fragmented. It was concluded that the Bone DNA Purification Kit manufactured by Eurx may be effective only in isolation of DNA from bones stored in good conditions.
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