Influence of histamine H3 receptors on pressor effect of leptin administered centrally in haemorrhage-shocked rats

• Authors: Karolina Jasikowska , Aleksandra Klose , Izabela Kozieł-Kokosińska , Jerzy Jochem

Title variants

PL

Wpływ receptorów histaminowych H3 na reakcję presyjną wywołaną przez leptynę podawaną ośrodkowo u szczurów we wstrząsie krwotocznym

• Languages of publication: EN

Abstracts

EN

INTRODUCTION: Leptin administered intracerebroventricularly (icv) evokes the resuscitating effect in haemorrhage-shocked rats, and the action is associated with activation of the histaminergic system. The aim of the present study was to examine the possible role of the histamine H3 receptor in leptin-evoked cardiovascular effects. MATERIALS AND METHODS: Studies were performed in male Wistar rats subjected to irreversible haemorrhagic shock (0% survival at 2 h) with a mean arterial pressure (MAP) of 20–25 mmHg that were anaesthetized with keta-mine/xylazine (100 mg/kg + 10 mg/kg, intraperitoneally). At 5 minutes of critical hypotension the animals were injec-ted icv with leptin (20 μg) or 0.9% NaCl (5 μl). RESULTS: Haemorrhage resulted in a decrease in pulse pressure (PP) and heart rate (HR). Leptin evoked long-lasting rises in MAP, PP and HR with 100% survival at 2 h. Pre-treatment with histamine H3 receptor inverse agonist/H4 partial agonist clobenpropit (42.5 nmol, icv), but not with H3 receptor neutral antagonist VUF 5681 (50 nmol, icv), inhibited MAP and PP changes evoked by leptin, however, without an influence on HR or survival rate at 2 h. CONCLUSIONS: The histamine H3 receptor can influence the centrally-acting leptin-induced resuscitating effect in haemorrhagic shock in rats.

PL

WSTĘP: Leptyna podawana do komory bocznej mózgu (icv) wywołuje efekt resuscytacyjny u szczurów we wstrząsie krwotocznym; w działaniu tym uczestniczy układ histaminergiczny. Celem obecnej pracy było zbadanie możliwego udziału receptorów histaminowych H3 w wywoływanym przez leptynę wpływie na układ krążenia we wstrząsie. MATERIAŁ I METODY: Badania przeprowadzono u szczurów, samców szczepu Wistar w znieczuleniu ogólnym przy użyciu ketaminy i ksylazyny (100 mg/kg + 10 mg/kg dootrzewnowo), u których wywołano nieodwracalny wstrząs krwotoczny ze średnim ciśnieniem tętniczym (MAP) 20–25 mmHg (wskaźnik przeżycia dwugodzinnego: 0%). W 5 min krytycznej hipotensji zwierzętom podawano icv leptynę (20 μg) bądź 0,9% roztwór NaCl (5 μl). WYNIKI: Utrata krwi prowadziła do obniżenia ciśnienia tętna (PP) i częstości rytmu serca (HR). Leptyna wywoływała długotrwałe wzrosty MAP, PP i HR, a także podwyższenie do 100% wskaźnika przeżycia dwugodzinnego. Premedykacja odwrotnym agonistą receptorów H3/częściowym agonistą receptorów H4 klobenpropitem (42,5 nmol, icv), ale nie neutralnym antagonistą receptorów H3 VUF 5681 (50 nmol, icv), hamowała zmiany MAP i PP wywoływane przez leptynę, jednak bez wpływu na wskaźnik przeżycia dwugodzinnego we wstrząsie. WNIOSKI: Receptory histaminowe H3 mogą wpływać na efekt resuscytacyjny wywoływany przez ośrodkowo działającą leptynę we wstrząsie krwotocznym u szczurów.

Keywords

EN

H3 receptor; haemorrhagic shock; histamina; histamine; leptin; leptyna; rats; receptor H3; szczury; wstrząs krwotoczny

Discipline

• MEDICINE: MEDICINE

• Publisher: Śląski Uniwersytet Medyczny w Katowicach
• Journal: Annales Academiae Medicae Silesiensis
• Year: 2015
• Volume: 69
• Pages: 132–137

Contributors

author

Karolina Jasikowska

• Department of Basic Medical Sciences, School of Public Health in Bytom, Medical University of Silesia

author

Aleksandra Klose

• Department of Basic Medical Sciences, School of Public Health in Bytom, Medical University of Silesia

author

Izabela Kozieł-Kokosińska

• Department of Basic Medical Sciences, School of Public Health in Bytom, Medical University of Silesia

author

Jerzy Jochem

• Katedra i Zakład Podstawowych Nauk Medycznych Wydziału Zdrowia Publicznego Śląskiego Uniwersytetu Medycznego w Katowicach, ul. Piekarska 18, 41-902 Bytom, tel. [phone]

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• Document Type: article