STRUCTURAL AND BIOLOGICAL CHARACTERISTICS OF SELF-ORGANISING CHITOSAN HYDROGELS

• Authors: Katarzyna Pieklarz , Michał Tylman , Zofia Modrzejewska , Grzegorz Galita , Ireneusz Majsterek
• Languages of publication: EN

Abstracts

EN

Creating innovative methods of treatment and regeneration of damaged tissues or organs is a key challenge of the twenty-first century. The aim of this study was to determine the possibility of producing and characterising the properties of self-organising chitosan hydrogels prepared with the use of chitosan lactate/chloride and disodium hydrogen phosphate dodecahydrate as a cross-linking agent. The structure and supramolecular architecture of the biomaterials were evaluated by Fourier-transform infrared spectroscopy and polarised optical microscopy. Biological studies assessed cytotoxicity by contact with a human colon adenocarcinoma cell line. The colourimetric resazurin assay showed that the obtained chitosan hydrogels are non-cytotoxic materials. Thus, self-organising biomaterials hold great promise for application in tissue engineering.

Keywords

EN

biomaterials; chitosan; cytotoxicity; self-organising hydrogels; structural properties; tissue engineering

• Publisher: Sieć Badawcza Łukasiewicz - Polskie Towarzystwo Chitynowe
• Journal: Progress on Chemistry and Application of Chitin and its Derivatives
• Year: 2021
• Volume: 26
• Pages: 191-199

Contributors

author

Katarzyna Pieklarz

• Faculty of Process and Environmental Engineering Lodz University of Technology

author

Michał Tylman

• PGE Gornictwo i Energetyka Konwencjonalna S.A.

author

Zofia Modrzejewska

• Faculty of Process and Environmental Engineering Lodz University of Technology,

author

Grzegorz Galita

• Department of Clinical Chemistry and Biochemistry Medical University of Lodz

author

Ireneusz Majsterek

• Department of Clinical Chemistry and Biochemistry Medical University of Lodz

References

• Fahy N.; (2007) Future EU strategy on health services. Clinical Medicine, 7 (1), 16–18. DOI: 10.7861/clinmedicine.7-1-16.
• Winiarska-Brodowska M.; (2015) Strategie komunikacyjne Unii Europejskiej w dziedzinie zdrowia. Zeszyty Prasoznawcze, 2 (222), 440–448. DOI: 10.4467/2299-6362PZ.15.031.4141.
• Zalewska M.; (2013) Analiza wybranych wskaźników zdrowia publicznego w świetle strategii zrównoważonego rozwoju w krajach UE. Problemy Zarządzania, 11 (1), 53–71. DOI: 10.7172/1644-9584.41.4.
• Langlotz F., Keeve E.; (2003) Minimally invasive approaches in orthopaedic surgery. Minimally Invasive Therapy & Allied Technologies, 12 (1-2), 19–24. DOI:10.1080/13645700310001559.
• Thaeter M., Kobbe P., Verhaven E., Pape H.C.; (2016) Minimally Invasive Techniques in Orthopedic Trauma. Current Trauma Reports, 2, 232–237. DOI:10.1007/s40719-016-0066-7.
• Kaźnica A., Joachimiak R., Drewa T., Rawo T., Deszczyński J.; (2007) New trends in tissue engineering. Arthroscopy and Joint Surgery, 3 (3), 11–16.
• Castells-Sala C., Alemany-Ribes M., Fernández-Muiños T., Recha-Sancho L., López-Chicón P., Aloy-Reverté C., Caballero-Camino J., Márquez-Gil A., Semino C.E.; (2013) Current Applications of Tissue Engineering in Biomedicine. Journal of Biochips & Tissue Chips, S2:004. DOI: 10.4172/2153-0777.S2-004.
• Sharma P., Kumar P., Sharma R., Bhatt V.D., Dhot P.S.; (2019) Tissue Engineering; Current Status & Futuristic Scope. Journal of Medicine and Life, 12 (3), 225–229. DOI:10.25122/jml-2019-0032.
• Kamolz L.P., Koch H., Kasper C.; (2013) Tissue engineering and its potential use in surgery. European Surgery, 45, 120–121. DOI: 10.1007/s10353-013-0216-x.
• Pieklarz K., Tylman M., Modrzejewska Z.; (2018) Applications of chitosangraphene oxide nanocomposites in medical science: A review. Progress on Chemistry and Application of Chitin and its Derivatives, XXIII, 5–24. DOI:10.15259/PCACD.23.01
• Pieklarz K., Tylman M., Modrzejewska Z.; (2020) Current Progress in Biomedical Applications of Chitosan-Carbon Nanotube Nanocomposites: A Review. Mini-Reviews in Medicinal Chemistry, 20 (16), 1619–1632. DOI:10.2174/1389557520666200513120407
• Büchel K.H., Moretto H.H., Werner D.; (2008) Industrial Inorganic Chemistry, 2nd edn. Wiley, New York.
• Boumpa T., Tsioulpas A., Grandison A.S., Lewis M.J.; (2007) Effects of phosphates and citrates on sediment formation in UHT goats’ milk. Journal of Dairy Research, 75 (2), 160–166. DOI: 10.1017/S0022029908003166.
• Aronson J.K. (eds); (2016) Meyler’s Side Effects of Drugs. The International Encyclopedia of Adverse Drug Reactions and Interactions., 16th edn. Elsevier Science.
• Khan S.A., Khan S.B., Khan L.U., Farooq A., Akhtar K., Asiri A.M.; (2018) Fourier Transform Infrared Spectroscopy: Fundamentals and Application in Functional Groups and Nanomaterials Characterization. In: Sharma S. (eds) Handbook of Materials Characterization. Springer, Cham.
• Portia S.A.U., Jayanthi K., Ramamoorthy K.; (2014) Growth and characterization of pure and sodium hydrogen phosphate mixed with potassium dihydrogen phosphate crystal by using slow evaporation technique. American Journal of Biological and Pharmaceutical Research, 1 (2), 77–82.
• Trivedi M.K., Branton A., Trivedi D., Nayak G., Bairwa K., Jana S.; (2015) Spectroscopic Characterization of Disodium Hydrogen Orthophosphate and Sodium Nitrate after Biofield Treatment. Journal of Chromatography and Separation Techniques, 6 (5). DOI: 10.4172/2157-7064.1000282.
• Pieklarz K., Tylman M., Modrzejewska Z.; (2020) Preparation and characterization of a new generation of chitosan hydrogels containing pyrimidine ribonucleotides. Progress on Chemistry and Application of Chitin and its Derivatives, XXV, 192–200. DOI:10.15259/PCACD.25.015
• Pieklarz K., Galita G., Tylman M., Maniukiewicz W., Kucharska E., Majsterek I., Modrzejewska Z.; (2021) Physico-Chemical Properties and Biocompatibility of Thermosensitive Chitosan Lactate and Chitosan Chloride Hydrogels Developed for Tissue Engineering Application. Journal of Functional Biomaterials, 12 (2), 37. DOI:10.3390/jfb12020037
• Delly J.G.; (2008) Essentials of Polarized Light Microscopy, 5th edn. College of Microscopy, Westmont.
• Rigon R.B., Oyafuso M.H., Fujimura A.T., Gonçalez M.L., Haddad do Prado A., Gremião M.P.D., Chorilli M.; (2015) Nanotechnology-Based Drug Delivery Systems for Melanoma Antitumoral Therapy: A Review. BioMed Research International. DOI:10.1155/2015/841817

• Document Type: article