Preferences help
enabled [disable] Abstract
Number of results
2016 | 42 | 73-86
Article title

Influence of magnetic energy on protein contents in the fifth instar larvae of silkworm, Bombyx mori (L) (Race: PM x CSR2)

Title variants
Languages of publication
The fifth instar larvae of multivoltine crossbreed race (PM x CSR2) of silkworm, Bombyx mori (L) were exposed to the magnetic energy of various strengths (1000, 2000, 3000 and 4000 Gauss magnetic field). The magnetization of fifth instar larvae was carried out on the first four days, for half an hour for each day before first feeding. Bioassay of total proteins was carried out on the fifth day of fifth instars. The attempt reveals influence of magnetization of Bombyx mori larvae on the total protein content level in the silk glands, fat bodies and haemolymph. The total protein content was increased with increase in the strength of magnetic field from 1000 to 4000 Gauss magnetic field. The larvae magnetized with 4000 Gauss magnetic field were found with sustained or decreased in total protein contents. Silk gland total proteins were increased from 5.901 to 17.481 percent. Total proteins of fat bodies were increased from 18 to 46.517 percent. And the total proteins of haemolymph were increased from 16.606 to 33.588 percent. Magnetization may have had influence on the increase in the levels of amino acids followed by accelerated rate of protein synthesis in the fifth instar larvae of silkworm, Bombyx mori (L) (Race: PM x CSR2). Magnetic energy should be utilized as efficiently as possible for the progression of growth of larval instars of silkworm, Bombyx mori (L).
Physical description
  • Zulal Bhilajirao Patil College, Z. B. Patil Road, Deopur, Dhule – 424002, MS, India
  • Research Group and Science Association, Shardabai Pawar Mahila Mahavidyalaya, Hardanagar, Tal. Baramati, Dist. Pune – 413115, India
  • Research Group and Science Association, Shardabai Pawar Mahila Mahavidyalaya, Hardanagar, Tal. Baramati, Dist. Pune – 413115, India
  • Research Group and Science Association, Shardabai Pawar Mahila Mahavidyalaya, Hardanagar, Tal. Baramati, Dist. Pune – 413115, India
  • [1] Verma, A.N. and A.S. Atwal, 1968. Effect of temperature and food on the development and silk production of Bombyx mori. L. Beitr. Entomol., 18(1&2): 249-258.
  • [2] Upadhyay, V.B. and A.B. Mishra, 2002. Influence of relative humidity on the nutritive potential of mulberry silkworm Bombyx mori L. larvae. J. Adv. Zool., 23(1): 54-58.
  • [3] Jolly, M.S., S.S. Sinha and J.L. Razdan, 1971. Influence of temperature and photoperiod on the termination of pupal diapouse in taser silkworm, Anthearea mylita. Indian J. Insect Physiol., 17: 743-760.
  • [4] Kanarev, G. and G.T. Cham, 1985. Effect of laser irradiation of silkworm eggs on development and productivities. Zhiotnov and Nauki, 229: 47-53.
  • [5] Patnev, T.P. and M.I. Mankova, 1986. Direct and indirect effect of a constant magnetic field on biological objects. Kosm. Biol. Aviakosm. Med., 20: 73-76.
  • [6] Chaugale, A.K., 1993. Effect of magnetic energy on silkworm development and silk production. Ph.D. Thesis, Shivaji University, Kohlapur, India.
  • [7] Udinsteve, N.A. and V.A. Moraz, 1982. Function of hypophysial adrenal system under the effect of power frequency variable magnetic field of different recime. Gig. Tr. Prof. Zobl., 12: 54-66.
  • [8] Conely, C.C., W.J. Mills and A.G. Patricia, 1966. Enzymes activities in macrophages from animals exposed to a very low magnetic field. III International Biomagnetic Symposium, University of Illinosis, Chicago, pp: 13-15.
  • [9] Pittman, U.J., 1965. Magnetism and plant growth III. Effect on germination and early growth of corns and beans. Canadian Journal of Plant Sci., 45: 549-555.
  • [10] Ferment, J., 1994. Effect of high magnetic field on biological reactions. Bioeng., 77: 453-456.
  • [11] Byers, G.M., K.F. Clark and G.C. Thompson, 1998. Effect of pulsed electro magnetic stimulation on the facial nerve regeneration. Archive of Otolaryngology Head and Neck Surgery, 124(4): 383-389.
  • [12] Darendetiler, M.A., A. Darendetiler and P.M. Sinclair, 1997. Effect of static and pulsed magnetic field on bone healing. International J. Orthodont, Orthognath Surgery, 12(1): 43-53.
  • [13] Tripathi, S.K. and V.B. Upadhyay, 2005. Magnetization of eggs influences the incubation period of multivoltine mulberry silkworm (Bombyx mori Linn.) Eggs. J. Adv. Zool., 26(1): 24-28.
  • [14] Upadhyay, V.B. and S.K. Tripathi, 2006. Effect of the magnetization of eggs on the silk producing potential of multivoltine mulberry silkworm (Bombyx mori Linn.). Sericologia, 46(3): 269-278.
  • [15] Tripathi, S.K., S.K. Shukla and V.B. Upadhyay, 2012. Impact of magnetization of eggs on the free amino acids content in the silk gland, fat body and haemolymph of Bombyx mori var. nistary larvae. World J. Zool., 7(1): 47-54.
  • [16] Krishnaswami, S., M.N. Narsimhanna, S.K. Suryanarayan and R. Kumar, 1973. Sericulture manual-2. Silkworm rearing. F.A.O. Agric. Seroes. Bull Rome., 15(2): 1-131.
  • [17] Lowry, O.H., N.J. Rosenbrought, A.L. Farr and R.J. Randall, 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem., 193: 265-275.
  • [18] Singh, D.K. and R.A. Agarwal, 1989. Toxicity of piperanyl butaxide carbonyl synergism on the snail Lymnaea accuminata, International Review Dergesamtem Hydrobiologic, 74: 689-699.
  • [19] Sokal R.R. and F.J. Rohlf, 1973. Introduction to Biostatistics. W.H. Freeman and co, San Franscisco CA, USA, pp. 185-207.
  • [20] Arora, P.N. and P.K. Malhan, 2004. Biostatistics. Himalaya Publishing House, Girgaon, Mumbai, pp: 137-166.
  • [21] Fllipovich, Y.B. and S.M. Klunova, 1990. Relationship between the dynamic of free amino acids and the biosynthesis of silk protein in the silk gland of Bombyx mori L. Obsch. Biol., 28(6): 715-723.
  • [22] Terra, W.R., A.G. De Blanchi, A.G. Gambrini and F.J.S. Larra, 1973. Haemolymph amino acids and related compounds during cocoon production by the larvae of fly, Rhynchosciara americana. J. Insect Physiol., 19(11): 2097-2106.
  • [23] Prudhomme, J.C., P. Cauble, J.P. Garel and J. Dallie, 1985. Silk synthesis. In comparative Insect Physiology, Biochemistry and Pharmacol., pp. 571-594.
  • [24] Pittman, U.J. and D.P. Ormord, 1970. Physiological and chemical features of magnetically treated winter seeds and resulting seedling. Can. J. Plant Sci., 50: 211-217.
  • [25] Ishaaya, I., I. Moore and D. Joseph, 1971. Protease and amylase in larvae of the Egyptian cotton worm, Spodoptera litoralis. J. Insect Physiol., 17: 945-953.
  • [26] Suzuki, Y., 1977. Differentiation of silk gland: A model system for the differential gene action. In: Result and problems in cell differentiation. W.B. Beermann, (Ed.), Springer-Verlag, Berlin.
  • [27] Chaugale, A.K. and N.K. More, 1992. Effect of magnetization on the developmental period and cocoon characters of the silkworm, Bombyx mori. Indian J. Seric., 31(2): 115-122.
  • [28] Coupland, R.E., 1957. Observation on the normal histology and histochemistry of the fat body of locust (Schistocerca gregaria). J. Exp. Biol., 34: 206-290.
  • [29] Wigglesworth, V.B., 1965. The principle of Insect Physiology, Chapman and Hall. Londan., pp. 663-690.
  • [30] Chen, P.S., 1972. Advances in Insect Physiology. Academic Press, Londan, 3: 53.
  • [31] Shigematsu, H., 1960. Protein metabolism in fat body of silkworm Bombyx mori L. Bull. Seric. Exp. Sta. Japan, 16: 141-170.
  • [32] Price, G.M., 1969. Protein synthesis and nucleic acid metabolism in the fat body of larvae blowfly. J. Insect Physiol., 15: 931-944.
  • [33] Octel, R., 1930. The synthesis of different constituents in Cercopia silkworm. J. Insect Physiol., 17: 677-689.
  • [34] Chippendale, G.M., 1971. Metamorphic changes in fat body protein of Southwestern corn borer, Diatraea grandiosella. J. Insect Physiol., 16: 1057-1068.
  • [35] Patel, H., 1972. Concept of temperature adaptation of unchanging reaction of cold blooded animal. In Procer, C.L. ed. Physiological adaptation. Amer. Phsiol. Soc., pp. 50-78.
  • [36] Okasha, A.Y.K., 1964. Effect of high temperature on the Rhodinus prolixus (Stad.). Nature London, 204: 1221-1222.
  • [37] Collins, J.V. and A.E.R. Downe, 1970. Selective accumulation of haemolymph protein by the fat body of Galleria mellonella. J. Insect Physiol., 16(9): 1697-1708.
  • [38] Beament, J.W.L, J.E. Trehern and V.B. Wigglesworth, 1995. Amino acid and protein metabolism. In Advances in Insect Physiol., 3: 84-86.
  • [39] Terra, W.R., C. Ferreora and A.G. De Balanchi, 1975. Distribution of nutrients reserves during spinning of larvae of fly Rhynchosciara americana. J. Insect Physiol., 21(8): 1501-1510.
  • [40] Digley, F. and J.M. Smith, 1969. Temperature acclimation in Drosophila melanogaster. J. Insect Physiol., 14: 1185-1194.
  • [41] Young, W., 1969. Magnetic field and in-situ acetycholine esterase in the vagal heart system, 79-102. In Biological effect of magnetic field (Ed. Barnothyet ) and Plenum Press.
  • [42] Vitthalrao B. Khyade; Patil, S.B; Khyade,, S.V and Bhavane, G.P (2002). Influence of acetone maceratives of Vitisvinifera on the larval parameters of silkworm Bombyxmori (L). Indian journal of comparative animal physiology 20: 14-18.
  • [43] Vitthalrao B. Khyade; Patil, S.B; Khyade, S.V and Bhavane, G.P (2003). Influence of acetone macerative of Vitisvinifera on the economic parameters of silkworm Bombyx mori (L). Indian journal of comparative animal physiology. 21: 28-32.
  • [44] Vitthalrao B. Khyade and Jiwan P. Sarwade (2013). Utilization of Retinol through the topical application to the fifth instar larvae of the silkworm, Bombyx mori (L) (Race : PM x CSR2) for qualitative improvement of the economic parameters. International Journal of Advanced Life Sciences 6 (5): 532-537.
  • [45] Vitthalrao B. Khyade; Karel Slama; Rajendra D. Pawar and Sanjay V. Deshmukh (2015). Influence of Various Concentrations of Acetone Solution of Retinol on Pattern of Chitin Deposition in the Integument of Fifth Instar Larvae of Silkworm, Bombyx Mori (L) (Pm X Csr2). Journal of Applicable Chemistry (4)15: 1434-1445.
  • [46] Gajanan B. Zore; Archana D. Thakre; Sitaram Jadhavand S. Mohan Karuppayil (2011). Terpenoids inhibit Candida albicans (L) growth by affecting membrane integrity and arrest cell cycle. Phytomedicine 18: 1181-1190.
  • [47] Vitthalrao B. Khyade and Anil N. Shendge (2012). Influence of Aloe vera (L) herbal formulation on the larval characters and economic parameters of silkworm, Bombyx mori (L) (Race : PM x CSR2). The Ecoscan Special Issue, 1: 321-326.
  • [48] Sucheta S. Doshi; Anil N. Shendage and Vitthalrao B. Khyade (2016). The monoterpene compounds for juvenile hormone activity through changes in pattern of chitin deposition in the integument of fifth instar larvae of silkworm, Bombyx mori (L) (PM x CSR2) World Scientific News 37 (2016) 179-201.
  • [49] Vitthalrao B. Khyade and Atharv Atul Gosavi (2016).Utilization of mulberry leaves treated with seed powder cowpea, Vigna unguiculata (L) for feeding the fifth instar larvae of silkworm, Bombyx mori (L) (Race: PM x CSR2). World Scientific News 40 (2016) 147-162.
  • [50] Khyade, V. B. (2004). Influence of juvenoids on silk worm, Bombyx mori (L). Ph.D. Thesis, Shivaji University, Kolhapur, India
  • [51] Khyade, V. B. and Ganga V. Mhamane (2005). Vividh Vanaspati Arkancha Tuti Reshim Kitak Sangopanasathi Upyojana. Krishi Vdnyan. 4: 18-22
  • [52] Khyade, V. B.; Poonam B. Patil; M. Jaybhay; Rasika R. Gaikwad; Ghantaloo,U. S. ;Vandana D. Shinde; Kavita H. Nimbalkar and Sarwade, J. P. (2007 ). Use of digoxin for improvement of economic parameters in silk worm, Bombyx mori (L).Bioinfrmatics (Zoological Society of India) Parent, A; Carpenter MB (1995). "Ch. 1". Carpenter's Human Neuroanatomy. Williams & Wilkins. ISBN 978-0-683-06752-1.
  • [53] Kristin L. Bigos, Ahmad R. Hariri, Daniel R. Weinberger (2015). Neuroimaging Genetics: Principles and Practices. Oxford University Press. p. 157. ISBN 0199920222. Retrieved January 2, 2016.
  • [54] Cosgrove, KP; Mazure CM; Staley JK (2007). "Evolving knowledge of sex differences in brain structure, function, and chemistry". Biol Psychiat 62 (8): 847-855. doi:10.1016/j.biopsych.2007.03.001. PMC 2711771. PMID 17544382.
  • [55] Azevedo, F.A.C., Carvalho, L.R.B., Grinberg, L.T., Farfel, J.M., Ferretti, R.E.L., Leite, R.E.P., Filho, W.J., Lent, R., Herculano-Houzel, S. (2009). "Equal numbers of neuronal and nonneuronal cells make the human brain an isometrically scaled-up primate brain." Journal of Comparative Neurology 513(5): 532-541. doi:10.1002/cne.21974.PMID 19226510.
  • [56] Kandel, ER; Schwartz JH; Jessel TM (2000). Principles of Neural Science. McGraw-Hill Professional. p. 324. ISBN 978-0-8385-7701-1.
  • [57] Jones R (2012). "Neurogenetics: What makes a human brain?". Nature Reviews Neuroscience 13(10): 655. doi:10.1038/nrn3355. PMID 22992645.
  • [58] From the National Library of Medicine's Visible Human Project. In this project, two human cadavers (from a man and a woman) were frozen and then sliced into thin sections, which were individually photographed and digitized. The slice here is taken from a small distance below the top of the brain, and shows the cerebral cortex (the convoluted cellular layer on the outside) and the underlying white matter, which consists of myelinated fiber tracts traveling to and from the cerebral cortex.
  • [59] Swaminathan, Nikhil (29 April 2008). "Why Does the Brain Need So Much Power?". Scientific American. Scientific American, a Division of Nature America, Inc. Retrieved 19 November 2010.
  • [60] Quistorff, Bjørn; Secher, Niels; Van Lieshout, Johanne (July 24, 2008). "Lactate fuels the human brain during exercise". The FASEB Journal 22(10): 3443-3449. doi:10.1096/fj.08-106104. Retrieved May 9, 2011.
  • [61] Obel, LF; Müller, MS; Walls, AB; Sickmann, HM; Bak, LK; Waagepetersen, HS; Schousboe, A (2012). "Brain glycogen-new perspectives on its metabolic function and regulation at the subcellular level.". Frontiers in neuroenergetics 4: 3. doi:10.3389/fnene.2012.00003. PMC 3291878. PMID 22403540.
  • [62] Clark, DD; Sokoloff L (1999). Siegel GJ, Agranoff BW, Albers RW, Fisher SK, Uhler MD, eds. Basic Neurochemistry: Molecular, Cellular and Medical Aspects. Philadelphia: Lippincott. pp. 637-670. ISBN 978-0-397-51820-3.
  • [63] Dorland's (2012). Dorland's Illustrated Medical Dictionary (32nd ed.). Elsevier. p. 784. ISBN 978-1-4160-6257-8.
  • [64] Easton, J. D. Albers; et al. (2009). "Definition and evaluation of transient ischemic attack: a scientific statement for healthcare professionals from the American Heart Association/American Stroke Association Stroke Council; Council on Cardiovascular Surgery and Anesthesia; Council on Cardiovascular Radiology and Intervention; Council on Cardiovascular Nursing; and the Interdisciplinary Council on Peripheral Vascular Disease. The American Academy of Neurology affirms the value of this statement as an educational tool for neurologists". Stroke 40(6): 2276 2293. doi:10.1161/STROKEAHA.108.192218.PMID 19423857.
Document Type
Publication order reference
YADDA identifier
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.